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Moxibustion regulates inflammatory mediators and colonic mucosal barrier in ulcerative colitis rats.
World J Gastroenterol. 2016 Feb 28; 22(8):2566-75.WJ

Abstract

AIM

To observe the efficacy and mechanism of grain-sized moxibustion at different acupoints in a rat model of ulcerative colitis (UC).

METHODS

Sprague-Dawley rats were randomly divided into control, UC model, grain-sized moxibustion at a single acupoint (CV 12), grain-sized moxibustion at two acupoints (CV 12 and CV 4), grain-sized moxibustion at three acupoints (CV 12, CV 4, and ST 36), and medication groups (n = 8/group). The UC model was established by enema of trinitrobenzene sulfonic acid. Direct moxibustion was used once a day for 7 d. Disease activity index (DAI) was evaluated before and after the treatment. Morphologic changes of intestinal tissue were observed under an optical microscope. The expression of tumor necrosis factor (TNF)-α and p38 mitogen-activated protein kinase (p38MAPK) in colonic tissue was detected using Western blot, and the levels of occludin and zonula occludens-1 (ZO-1) mRNAs were detected using reverse transcription PCR.

RESULTS

Compared with the control group, the intestinal mucosae were incomplete in the model group, glandular structures were irregular, and submucosae were edematous, hyperemic, and infiltrated with inflammatory cells. The DAI scores and expression of TNF-α and p38MAPK were increased significantly in the model group compared to controls (Ps < 0.01), while the mRNA levels of occludin and ZO-1 were reduced significantly (Ps < 0.01). Compared with the model group, colonic mucosa and the arrangement of glands were complete and regular in the treatment groups. DAI scores and the expression of TNF-α and p38MAPK were reduced significantly in moxibustion groups compared to controls (Ps < 0.01), while the mRNA levels of occludin and ZO-1 were increased significantly (Ps < 0.01). The improvements in the above indices in the three acupoints group and the medication group were superior to those in the single and two acupoints groups (all P < 0.05).

CONCLUSION

Reduction of TNF-α and p38MAPK and increased expression of occludin and ZO-1 in colonic tissue represent a potential mechanism for improved intestinal mucosal tissue repair with grain-sized moxibustion.

Authors+Show Affiliations

Tie-Ming Ma, Na Xu, Xian-De Ma, Zeng-Hua Bai, Xing Tao, Hong-Chi Yan, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning Province, China.Tie-Ming Ma, Na Xu, Xian-De Ma, Zeng-Hua Bai, Xing Tao, Hong-Chi Yan, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning Province, China.Tie-Ming Ma, Na Xu, Xian-De Ma, Zeng-Hua Bai, Xing Tao, Hong-Chi Yan, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning Province, China.Tie-Ming Ma, Na Xu, Xian-De Ma, Zeng-Hua Bai, Xing Tao, Hong-Chi Yan, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning Province, China.Tie-Ming Ma, Na Xu, Xian-De Ma, Zeng-Hua Bai, Xing Tao, Hong-Chi Yan, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning Province, China.Tie-Ming Ma, Na Xu, Xian-De Ma, Zeng-Hua Bai, Xing Tao, Hong-Chi Yan, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning Province, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26937144

Citation

Ma, Tie-Ming, et al. "Moxibustion Regulates Inflammatory Mediators and Colonic Mucosal Barrier in Ulcerative Colitis Rats." World Journal of Gastroenterology, vol. 22, no. 8, 2016, pp. 2566-75.
Ma TM, Xu N, Ma XD, et al. Moxibustion regulates inflammatory mediators and colonic mucosal barrier in ulcerative colitis rats. World J Gastroenterol. 2016;22(8):2566-75.
Ma, T. M., Xu, N., Ma, X. D., Bai, Z. H., Tao, X., & Yan, H. C. (2016). Moxibustion regulates inflammatory mediators and colonic mucosal barrier in ulcerative colitis rats. World Journal of Gastroenterology, 22(8), 2566-75. https://doi.org/10.3748/wjg.v22.i8.2566
Ma TM, et al. Moxibustion Regulates Inflammatory Mediators and Colonic Mucosal Barrier in Ulcerative Colitis Rats. World J Gastroenterol. 2016 Feb 28;22(8):2566-75. PubMed PMID: 26937144.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Moxibustion regulates inflammatory mediators and colonic mucosal barrier in ulcerative colitis rats. AU - Ma,Tie-Ming, AU - Xu,Na, AU - Ma,Xian-De, AU - Bai,Zeng-Hua, AU - Tao,Xing, AU - Yan,Hong-Chi, PY - 2015/09/01/received PY - 2015/10/30/revised PY - 2015/11/30/accepted PY - 2016/3/4/entrez PY - 2016/3/5/pubmed PY - 2017/1/25/medline KW - Different acupoints KW - Grain-sized moxibustion KW - Inflammatory factors KW - Intestinal mucosal barrier KW - Ulcerative colitis SP - 2566 EP - 75 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 22 IS - 8 N2 - AIM: To observe the efficacy and mechanism of grain-sized moxibustion at different acupoints in a rat model of ulcerative colitis (UC). METHODS: Sprague-Dawley rats were randomly divided into control, UC model, grain-sized moxibustion at a single acupoint (CV 12), grain-sized moxibustion at two acupoints (CV 12 and CV 4), grain-sized moxibustion at three acupoints (CV 12, CV 4, and ST 36), and medication groups (n = 8/group). The UC model was established by enema of trinitrobenzene sulfonic acid. Direct moxibustion was used once a day for 7 d. Disease activity index (DAI) was evaluated before and after the treatment. Morphologic changes of intestinal tissue were observed under an optical microscope. The expression of tumor necrosis factor (TNF)-α and p38 mitogen-activated protein kinase (p38MAPK) in colonic tissue was detected using Western blot, and the levels of occludin and zonula occludens-1 (ZO-1) mRNAs were detected using reverse transcription PCR. RESULTS: Compared with the control group, the intestinal mucosae were incomplete in the model group, glandular structures were irregular, and submucosae were edematous, hyperemic, and infiltrated with inflammatory cells. The DAI scores and expression of TNF-α and p38MAPK were increased significantly in the model group compared to controls (Ps < 0.01), while the mRNA levels of occludin and ZO-1 were reduced significantly (Ps < 0.01). Compared with the model group, colonic mucosa and the arrangement of glands were complete and regular in the treatment groups. DAI scores and the expression of TNF-α and p38MAPK were reduced significantly in moxibustion groups compared to controls (Ps < 0.01), while the mRNA levels of occludin and ZO-1 were increased significantly (Ps < 0.01). The improvements in the above indices in the three acupoints group and the medication group were superior to those in the single and two acupoints groups (all P < 0.05). CONCLUSION: Reduction of TNF-α and p38MAPK and increased expression of occludin and ZO-1 in colonic tissue represent a potential mechanism for improved intestinal mucosal tissue repair with grain-sized moxibustion. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/26937144/Moxibustion_regulates_inflammatory_mediators_and_colonic_mucosal_barrier_in_ulcerative_colitis_rats_ L2 - http://www.wjgnet.com/1007-9327/full/v22/i8/2566.htm DB - PRIME DP - Unbound Medicine ER -