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Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis.
Toxins (Basel). 2016 Feb 29; 8(3)T

Abstract

Taipans (Oxyuranus spp.) are elapids with highly potent venoms containing presynaptic (β) and postsynaptic (α) neurotoxins. O. temporalis (Western Desert taipan), a newly discovered member of this genus, has been shown to possess venom which displays marked in vitro neurotoxicity. No components have been isolated from this venom. We describe the characterization of α-elapitoxin-Ot1a (α-EPTX-Ot1a; 6712 Da), a short-chain postsynaptic neurotoxin, which accounts for approximately 30% of O. temporalis venom. α-Elapitoxin-Ot1a (0.1-1 µM) produced concentration-dependent inhibition of indirect-twitches, and abolished contractile responses to exogenous acetylcholine and carbachol, in the chick biventer cervicis nerve-muscle preparation. The inhibition of indirect twitches by α-elapitoxin-Ot1a (1 µM) was not reversed by washing the tissue. Prior addition of taipan antivenom (10 U/mL) delayed the neurotoxic effects of α-elapitoxin-Ot1a (1 µM) and markedly attenuated the neurotoxic effects of α-elapitoxin-Ot1a (0.1 µM). α-Elapitoxin-Ot1a displayed pseudo-irreversible antagonism of concentration-response curves to carbachol with a pA₂ value of 8.02 ± 0.05. De novo sequencing revealed the main sequence of the short-chain postsynaptic neurotoxin (i.e., α-elapitoxin-Ot1a) as well as three other isoforms found in O. temporalis venom. α-Elapitoxin-Ot1a shows high sequence similarity (i.e., >87%) with other taipan short-chain postsynaptic neurotoxins.

Authors+Show Affiliations

Monash Venom Group, Department of Pharmacology, Monash University, Clayton, VIC 3168, Australia. carmel.barber@gmail.com.Monash Venom Group, Department of Pharmacology, Monash University, Clayton, VIC 3168, Australia. rusdirusmili@iium.edu.my. Department of Basic Medical Sciences, Kulliyyah of Pharmacy, International Islamic University Malaysia, Bandar Indera Mahkota 23800, Malaysia. rusdirusmili@iium.edu.my.Monash Venom Group, Department of Pharmacology, Monash University, Clayton, VIC 3168, Australia. wayne.hodgson@monash.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26938558

Citation

Barber, Carmel M., et al. "Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin From the Venom of the Western Desert Taipan, Oxyuranus Temporalis." Toxins, vol. 8, no. 3, 2016.
Barber CM, Rusmili MR, Hodgson WC. Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis. Toxins (Basel). 2016;8(3).
Barber, C. M., Rusmili, M. R., & Hodgson, W. C. (2016). Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis. Toxins, 8(3). https://doi.org/10.3390/toxins8030058
Barber CM, Rusmili MR, Hodgson WC. Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin From the Venom of the Western Desert Taipan, Oxyuranus Temporalis. Toxins (Basel). 2016 Feb 29;8(3) PubMed PMID: 26938558.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis. AU - Barber,Carmel M, AU - Rusmili,Muhamad Rusdi Ahmad, AU - Hodgson,Wayne C, Y1 - 2016/02/29/ PY - 2015/12/22/received PY - 2016/02/19/revised PY - 2016/02/19/accepted PY - 2016/3/4/entrez PY - 2016/3/5/pubmed PY - 2016/12/15/medline KW - Oxyuranus temporalis KW - antivenom KW - postsynaptic neurotoxin KW - snake KW - α-Elapitoxin-Ot1a JF - Toxins JO - Toxins (Basel) VL - 8 IS - 3 N2 - Taipans (Oxyuranus spp.) are elapids with highly potent venoms containing presynaptic (β) and postsynaptic (α) neurotoxins. O. temporalis (Western Desert taipan), a newly discovered member of this genus, has been shown to possess venom which displays marked in vitro neurotoxicity. No components have been isolated from this venom. We describe the characterization of α-elapitoxin-Ot1a (α-EPTX-Ot1a; 6712 Da), a short-chain postsynaptic neurotoxin, which accounts for approximately 30% of O. temporalis venom. α-Elapitoxin-Ot1a (0.1-1 µM) produced concentration-dependent inhibition of indirect-twitches, and abolished contractile responses to exogenous acetylcholine and carbachol, in the chick biventer cervicis nerve-muscle preparation. The inhibition of indirect twitches by α-elapitoxin-Ot1a (1 µM) was not reversed by washing the tissue. Prior addition of taipan antivenom (10 U/mL) delayed the neurotoxic effects of α-elapitoxin-Ot1a (1 µM) and markedly attenuated the neurotoxic effects of α-elapitoxin-Ot1a (0.1 µM). α-Elapitoxin-Ot1a displayed pseudo-irreversible antagonism of concentration-response curves to carbachol with a pA₂ value of 8.02 ± 0.05. De novo sequencing revealed the main sequence of the short-chain postsynaptic neurotoxin (i.e., α-elapitoxin-Ot1a) as well as three other isoforms found in O. temporalis venom. α-Elapitoxin-Ot1a shows high sequence similarity (i.e., >87%) with other taipan short-chain postsynaptic neurotoxins. SN - 2072-6651 UR - https://www.unboundmedicine.com/medline/citation/26938558/Isolation_and_Pharmacological_Characterization_of_α_Elapitoxin_Ot1a_a_Short_Chain_Postsynaptic_Neurotoxin_from_the_Venom_of_the_Western_Desert_Taipan_Oxyuranus_temporalis_ L2 - https://www.mdpi.com/resolver?pii=toxins8030058 DB - PRIME DP - Unbound Medicine ER -