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[Analysis of ISSR Amplification Results of Six Species in Sect. Cruciata Gaudin].
Zhong Yao Cai. 2015 Jul; 38(7):1375-8.ZY

Abstract

OBJECTIVE

To identify and analyze the genetic relationship of four species of Gentiana (G. macrophylla, G. straminea, G. dahurica and G. crassicaulis) recorded as Gentianae Macrophyllae Radix in the Chinese Pharmacopoeia, and two other Gentiana species (G. officinalis and G. siphonantha) often used as substitutes by ISSR, in order to estimate the reasonability of G. officinalis and G. siphonantha used as substitutes from the DNA level.

METHODS

Eight primers ivere screened to amplify all the samples and agarose gel electrophoresis were analyzed. NTSYSpc-2. 10E software was used to calculate similarity coefficient and draw dendrogram. Results: Nine characteristic bands were found in different species on the ISSR fingerprints and which could be used to identify five species except G. dahurica. The substitute G. officinalis firstly clustered with G. dahurica and G. siphonantha showed closer genetic relationship with G. straminea and G. dahurica. G. crassicaulis showed a far genetic relationship with the other five species.

CONCLUSION

The dendrogram based on the ISSR data supports that G. officinalis and G. siphonantha can be used as substitutes of Gentianae Macrophyllae Radix.

Authors

Zhou WP
No affiliation info available
Zhang HJ
No affiliation info available
Wang YF
No affiliation info available
Wang ZZ
No affiliation info available
Cao XY
No affiliation info available

MeSH

DNA FingerprintingDNA PrimersDNA, PlantElectrophoresis, Agar GelGentianaPlants, Medicinal

Pub Type(s)

Journal Article

Language

chi

PubMed ID

26946833

Citation

Zhou, Wen-ping, et al. "[Analysis of ISSR Amplification Results of Six Species in Sect. Cruciata Gaudin]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 38, no. 7, 2015, pp. 1375-8.
Zhou WP, Zhang HJ, Wang YF, et al. [Analysis of ISSR Amplification Results of Six Species in Sect. Cruciata Gaudin]. Zhong Yao Cai. 2015;38(7):1375-8.
Zhou, W. P., Zhang, H. J., Wang, Y. F., Wang, Z. Z., & Cao, X. Y. (2015). [Analysis of ISSR Amplification Results of Six Species in Sect. Cruciata Gaudin]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 38(7), 1375-8.
Zhou WP, et al. [Analysis of ISSR Amplification Results of Six Species in Sect. Cruciata Gaudin]. Zhong Yao Cai. 2015;38(7):1375-8. PubMed PMID: 26946833.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Analysis of ISSR Amplification Results of Six Species in Sect. Cruciata Gaudin]. AU - Zhou,Wen-ping, AU - Zhang,Hui-juan, AU - Wang,Ya-fei, AU - Wang,Zhe-zhi, AU - Cao,Xiao-yan, PY - 2016/3/8/entrez PY - 2016/3/8/pubmed PY - 2016/5/14/medline SP - 1375 EP - 8 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 38 IS - 7 N2 - OBJECTIVE: To identify and analyze the genetic relationship of four species of Gentiana (G. macrophylla, G. straminea, G. dahurica and G. crassicaulis) recorded as Gentianae Macrophyllae Radix in the Chinese Pharmacopoeia, and two other Gentiana species (G. officinalis and G. siphonantha) often used as substitutes by ISSR, in order to estimate the reasonability of G. officinalis and G. siphonantha used as substitutes from the DNA level. METHODS: Eight primers ivere screened to amplify all the samples and agarose gel electrophoresis were analyzed. NTSYSpc-2. 10E software was used to calculate similarity coefficient and draw dendrogram. Results: Nine characteristic bands were found in different species on the ISSR fingerprints and which could be used to identify five species except G. dahurica. The substitute G. officinalis firstly clustered with G. dahurica and G. siphonantha showed closer genetic relationship with G. straminea and G. dahurica. G. crassicaulis showed a far genetic relationship with the other five species. CONCLUSION: The dendrogram based on the ISSR data supports that G. officinalis and G. siphonantha can be used as substitutes of Gentianae Macrophyllae Radix. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/26946833/[Analysis_of_ISSR_Amplification_Results_of_Six_Species_in_Sect__Cruciata_Gaudin]_ DB - PRIME DP - Unbound Medicine ER -