Tags

Type your tag names separated by a space and hit enter

[HPLC Specific Fingerprint of Alcohol Extract of Andrographis paniculata].
Zhong Yao Cai. 2015 Jul; 38(7):1505-8.ZY

Abstract

OBJECTIVE

To establish the specific fingerprint of alcohol extract of Andrographis paniculata by HPLC.

METHODS

The analysis was performed on Cosmosil 5C18 -MS-II (250 mm x 4. 6 mm, 5 µm) column, with gradient phase consisting of acetonitrile and water at the flow rate of 1. 0 mL/min. The UV detection wavelength was set at 225 nm, and column temperature was 30 °C.

RESULTS

The specific fingerprint chromatogram was established and seven common peaks were identified by comparison with the reference standards and LC-MS. The relative retention times were 1. 00 (No. 1, andrographolide), 1. 04 (No. 2, deoxyandrographoside), 1. 07 (No. 3, isoandrographolide), 1. 10 (No. 4, 14-deoxy-11, 12-didehydroandrographoside), 1. 50 (No. 5, neoandrographolide), 1. 75 (No. 6, deoxyandrographolide) and 1. 79 (No. 7, dehydroandrographolide).

CONCLUSION

The method is simple and reproducible with high precision, which can provide the basis for the quality control and evaluation of alcohol extract of Andrographis paniculata and its preparations.

Authors

Liu FF
No affiliation info available
Fan CL
No affiliation info available
Huang XJ
No affiliation info available
Wang GY
No affiliation info available
Wang Y
No affiliation info available
Ye WC
No affiliation info available

MeSH

AndrographisChromatography, High Pressure LiquidDiterpenesEthanolGlucosidesPlant ExtractsPlants, MedicinalTetrahydronaphthalenes

Pub Type(s)

Journal Article

Language

chi

PubMed ID

26946847

Citation

Liu, Fei-fei, et al. "[HPLC Specific Fingerprint of Alcohol Extract of Andrographis Paniculata]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 38, no. 7, 2015, pp. 1505-8.
Liu FF, Fan CL, Huang XJ, et al. [HPLC Specific Fingerprint of Alcohol Extract of Andrographis paniculata]. Zhong Yao Cai. 2015;38(7):1505-8.
Liu, F. F., Fan, C. L., Huang, X. J., Wang, G. Y., Wang, Y., & Ye, W. C. (2015). [HPLC Specific Fingerprint of Alcohol Extract of Andrographis paniculata]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 38(7), 1505-8.
Liu FF, et al. [HPLC Specific Fingerprint of Alcohol Extract of Andrographis Paniculata]. Zhong Yao Cai. 2015;38(7):1505-8. PubMed PMID: 26946847.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [HPLC Specific Fingerprint of Alcohol Extract of Andrographis paniculata]. AU - Liu,Fei-fei, AU - Fan,Chun-lin, AU - Huang,Xiao-jun, AU - Wang,Gui-yang, AU - Wang,Ying, AU - Ye,Wen-cai, PY - 2016/3/8/entrez PY - 2016/3/8/pubmed PY - 2016/5/14/medline SP - 1505 EP - 8 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 38 IS - 7 N2 - OBJECTIVE: To establish the specific fingerprint of alcohol extract of Andrographis paniculata by HPLC. METHODS: The analysis was performed on Cosmosil 5C18 -MS-II (250 mm x 4. 6 mm, 5 µm) column, with gradient phase consisting of acetonitrile and water at the flow rate of 1. 0 mL/min. The UV detection wavelength was set at 225 nm, and column temperature was 30 °C. RESULTS: The specific fingerprint chromatogram was established and seven common peaks were identified by comparison with the reference standards and LC-MS. The relative retention times were 1. 00 (No. 1, andrographolide), 1. 04 (No. 2, deoxyandrographoside), 1. 07 (No. 3, isoandrographolide), 1. 10 (No. 4, 14-deoxy-11, 12-didehydroandrographoside), 1. 50 (No. 5, neoandrographolide), 1. 75 (No. 6, deoxyandrographolide) and 1. 79 (No. 7, dehydroandrographolide). CONCLUSION: The method is simple and reproducible with high precision, which can provide the basis for the quality control and evaluation of alcohol extract of Andrographis paniculata and its preparations. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/26946847/[HPLC_Specific_Fingerprint_of_Alcohol_Extract_of_Andrographis_paniculata]_ DB - PRIME DP - Unbound Medicine ER -