Tags

Type your tag names separated by a space and hit enter

Comparison of 3 type VII collagen (C7) assays for serologic diagnosis of epidermolysis bullosa acquisita (EBA).
J Am Acad Dermatol. 2016 Jun; 74(6):1166-72.JA

Abstract

BACKGROUND

Serologic diagnosis of epidermolysis bullosa acquisita (EBA) relies on the detection of circulating autoantibodies to type VII collagen (C7).

OBJECTIVE

We sought to compare the diagnostic performances of a commercialized enzyme-linked immunosorbent assay (ELISA) using C7 noncollagenous (NC) domains (C7-NC1/NC2 ELISA) and indirect immunofluorescence (IIF) biochip test on NC1-C7-expressing transfected cells (IIFT), with a full-length-C7 ELISA developed in our laboratory.

METHODS

C7-NC1/NC2 ELISA, IIFT, and full-length-C7 ELISA were run on 77 nonselected consecutive EBA sera.

RESULTS

C7-NC1/NC2 ELISA, IIFT, and full-length-C7 ELISA were positive, respectively, for: 30%, 27%, and 65% of the 77 sera; 43%, 32%, and 80% of 44 sera labeling the salt-split-skin (SSS) floor (F) by IIF (SSS/F(+)); 9%, 22%, and 47% of 32 SSS/F(-) sera; 28%, 28%, and 58% of classic EBA; 41%, 41%, and 82% of inflammatory EBA; and 18%, 0%, and 55% of mucous-membrane-predominant EBA. Significant differences for all sera were found between: the 2 ELISAs for the 77 sera, SSS/F(+) and SSS/F(-) sera, and IIFT versus full-length-C7 ELISA.

LIMITATIONS

The retrospective design was a limitation.

CONCLUSION

C7-NC1/NC2 ELISA and IIFT sensitivities for serologic diagnoses of EBA were low. Full-length-C7 ELISA was significantly more sensitive and could serve as a reference test.

Authors+Show Affiliations

Department of Dermatology, APHP, Avicenne Hospital, Bobigny, France.Department of Immunology, Assistance Publique des Hôpitaux de Paris (APHP), Saint-Louis Hospital, Paris, France.Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1163, Institut Imagine, Paris, France; Université Paris Descartes-Sorbonne Paris Cité, Paris, France.Department of Dermatology, APHP, Avicenne Hospital, Bobigny, France.INSERM, U563, Purpan Hospital, Toulouse, France.Department of Dermatology, APHP, Avicenne Hospital, Bobigny, France.Department of Immunology, APHP, Bichat Hospital, Paris, France.Department of Histology, Unité de Formation et de Recherche (UFR) Léonard de Vinci, University Paris 13, Bobigny, France.Department of Histology, Unité de Formation et de Recherche (UFR) Léonard de Vinci, University Paris 13, Bobigny, France.Department of Dermatology, APHP, Avicenne Hospital, Bobigny, France.Department of Dermatology, APHP, Avicenne Hospital, Bobigny, France.Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1163, Institut Imagine, Paris, France; Université Paris Descartes-Sorbonne Paris Cité, Paris, France.Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1163, Institut Imagine, Paris, France; Université Paris Descartes-Sorbonne Paris Cité, Paris, France; Department of Genetics, APHP, Necker-Enfants Malades Hospital, Paris, France.Department of Dermatology, APHP, Avicenne Hospital, Bobigny, France; Department of Histology, Unité de Formation et de Recherche (UFR) Léonard de Vinci, University Paris 13, Bobigny, France; Department of Pathology, APHP, Avicenne Hospital, Bobigny, France. Electronic address: catherine.prost@aphp.fr.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

26947449

Citation

Seta, Vannina, et al. "Comparison of 3 Type VII Collagen (C7) Assays for Serologic Diagnosis of Epidermolysis Bullosa Acquisita (EBA)." Journal of the American Academy of Dermatology, vol. 74, no. 6, 2016, pp. 1166-72.
Seta V, Aucouturier F, Bonnefoy J, et al. Comparison of 3 type VII collagen (C7) assays for serologic diagnosis of epidermolysis bullosa acquisita (EBA). J Am Acad Dermatol. 2016;74(6):1166-72.
Seta, V., Aucouturier, F., Bonnefoy, J., Le Roux-Villet, C., Pendaries, V., Alexandre, M., Grootenboer-Mignot, S., Heller, M., Lièvre, N., Laroche, L., Caux, F., Titeux, M., Hovnanian, A., & Prost-Squarcioni, C. (2016). Comparison of 3 type VII collagen (C7) assays for serologic diagnosis of epidermolysis bullosa acquisita (EBA). Journal of the American Academy of Dermatology, 74(6), 1166-72. https://doi.org/10.1016/j.jaad.2016.01.005
Seta V, et al. Comparison of 3 Type VII Collagen (C7) Assays for Serologic Diagnosis of Epidermolysis Bullosa Acquisita (EBA). J Am Acad Dermatol. 2016;74(6):1166-72. PubMed PMID: 26947449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of 3 type VII collagen (C7) assays for serologic diagnosis of epidermolysis bullosa acquisita (EBA). AU - Seta,Vannina, AU - Aucouturier,Françoise, AU - Bonnefoy,Jonathan, AU - Le Roux-Villet,Christelle, AU - Pendaries,Valérie, AU - Alexandre,Marina, AU - Grootenboer-Mignot,Sabine, AU - Heller,Michel, AU - Lièvre,Nicole, AU - Laroche,Liliane, AU - Caux,Frédéric, AU - Titeux,Matthias, AU - Hovnanian,Alain, AU - Prost-Squarcioni,Catherine, Y1 - 2016/03/03/ PY - 2015/08/07/received PY - 2015/12/26/revised PY - 2016/01/01/accepted PY - 2016/3/8/entrez PY - 2016/3/8/pubmed PY - 2017/4/14/medline KW - autoimmune bullous disease KW - biochip KW - enzyme-linked immunosorbent assay KW - epidermolysis bullosa acquisita KW - indirect immunofluorescence KW - sensitivity KW - serologic diagnosis KW - type VII collagen SP - 1166 EP - 72 JF - Journal of the American Academy of Dermatology JO - J Am Acad Dermatol VL - 74 IS - 6 N2 - BACKGROUND: Serologic diagnosis of epidermolysis bullosa acquisita (EBA) relies on the detection of circulating autoantibodies to type VII collagen (C7). OBJECTIVE: We sought to compare the diagnostic performances of a commercialized enzyme-linked immunosorbent assay (ELISA) using C7 noncollagenous (NC) domains (C7-NC1/NC2 ELISA) and indirect immunofluorescence (IIF) biochip test on NC1-C7-expressing transfected cells (IIFT), with a full-length-C7 ELISA developed in our laboratory. METHODS: C7-NC1/NC2 ELISA, IIFT, and full-length-C7 ELISA were run on 77 nonselected consecutive EBA sera. RESULTS: C7-NC1/NC2 ELISA, IIFT, and full-length-C7 ELISA were positive, respectively, for: 30%, 27%, and 65% of the 77 sera; 43%, 32%, and 80% of 44 sera labeling the salt-split-skin (SSS) floor (F) by IIF (SSS/F(+)); 9%, 22%, and 47% of 32 SSS/F(-) sera; 28%, 28%, and 58% of classic EBA; 41%, 41%, and 82% of inflammatory EBA; and 18%, 0%, and 55% of mucous-membrane-predominant EBA. Significant differences for all sera were found between: the 2 ELISAs for the 77 sera, SSS/F(+) and SSS/F(-) sera, and IIFT versus full-length-C7 ELISA. LIMITATIONS: The retrospective design was a limitation. CONCLUSION: C7-NC1/NC2 ELISA and IIFT sensitivities for serologic diagnoses of EBA were low. Full-length-C7 ELISA was significantly more sensitive and could serve as a reference test. SN - 1097-6787 UR - https://www.unboundmedicine.com/medline/citation/26947449/Comparison_of_3_type_VII_collagen__C7__assays_for_serologic_diagnosis_of_epidermolysis_bullosa_acquisita__EBA__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0190-9622(16)00009-8 DB - PRIME DP - Unbound Medicine ER -