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Rapid screening and identification of phenolic antioxidants in Hydrocotyle sibthorpioides Lam. by UPLC-ESI-MS/MS.
Food Chem. 2016 Jul 15; 203:521-529.FC

Abstract

The aim of the study was to identify the phenolic compounds present in Hydrocotyle sibthorpioides (HS), Centella asiatica (CA) and Amaranthus viridis (AV) extracts and investigate their respective antioxidant activities. Herein, an ultra-high pressure liquid chromatography-mass spectrometer (UPLC-MS/MS) analytical method has been developed for the separation, and systematic characterization of the phenolic compounds in HS, CA and AV extracts and was compared along with ten standard phenolic compounds. Additionally, in vitro antioxidant activity of the phenolic compounds was also determined. The HS extract revealed excellent antioxidant activity such as 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity (IC50=19.7 ± 1.2 μg/mL), total reduction capability (0.169 ± 0.003 at 100 μg/mL), nitric oxide radical scavenging activity (IC50=39.33 ± 3.2 μg/mL), metal chelating activity (IC50=56.51 ± 3.6 μg/mL) and inhibition of lipid peroxidation (IC50=12.34 ± 2.3 μg/mL) as compared to CA and AV extracts. Furthermore, catechin, epicatechin, quercetin and chlorogenic acid were found to be the major components responsible for the antioxidant activity of the HS extract as evidenced from UPLC-MS/MS. Taken together, this study demonstrates the promising antioxidant properties of the HS extract, which can further be utilized in various pharmaceutical, food, and agricultural applications.

Authors+Show Affiliations

Biochemistry Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, Assam, India.Drug Discovery Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, Assam, India.Drug Discovery Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, Assam, India.Biochemistry Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, Assam, India. Electronic address: rajiasst@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26948646

Citation

Kumari, Sima, et al. "Rapid Screening and Identification of Phenolic Antioxidants in Hydrocotyle Sibthorpioides Lam. By UPLC-ESI-MS/MS." Food Chemistry, vol. 203, 2016, pp. 521-529.
Kumari S, Elancheran R, Kotoky J, et al. Rapid screening and identification of phenolic antioxidants in Hydrocotyle sibthorpioides Lam. by UPLC-ESI-MS/MS. Food Chem. 2016;203:521-529.
Kumari, S., Elancheran, R., Kotoky, J., & Devi, R. (2016). Rapid screening and identification of phenolic antioxidants in Hydrocotyle sibthorpioides Lam. by UPLC-ESI-MS/MS. Food Chemistry, 203, 521-529. https://doi.org/10.1016/j.foodchem.2016.02.101
Kumari S, et al. Rapid Screening and Identification of Phenolic Antioxidants in Hydrocotyle Sibthorpioides Lam. By UPLC-ESI-MS/MS. Food Chem. 2016 Jul 15;203:521-529. PubMed PMID: 26948646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid screening and identification of phenolic antioxidants in Hydrocotyle sibthorpioides Lam. by UPLC-ESI-MS/MS. AU - Kumari,Sima, AU - Elancheran,R, AU - Kotoky,Jibon, AU - Devi,Rajlakshmi, Y1 - 2016/02/15/ PY - 2015/08/06/received PY - 2015/12/21/revised PY - 2016/02/13/accepted PY - 2016/3/8/entrez PY - 2016/3/8/pubmed PY - 2016/11/1/medline KW - (+)-Catechin (PubChem CID: 107957) KW - (–)-Epicatechin (PubChem CID: 72276) KW - Antioxidants KW - Apigenin (PubChem CID: 5280443) KW - Biochanin A (PubChem CID: 5280373) KW - Caffeine (PubChem CID: 2519) KW - Chlorogenic acid (PubChem CID: 1794427) KW - Flavonoids KW - Phenolic acids KW - Polyphenols KW - Quercetin (PubChem CID: 5280343) KW - Rosmarinic acid (PubChem CID: 5281792) KW - Sinapic acid (PubChem CID: 637775) KW - Trans-cinnamic acid (PubChem CID: 444539) KW - Tyrosol (PubChem CID: 10393) KW - UHPLC–MS SP - 521 EP - 529 JF - Food chemistry JO - Food Chem VL - 203 N2 - The aim of the study was to identify the phenolic compounds present in Hydrocotyle sibthorpioides (HS), Centella asiatica (CA) and Amaranthus viridis (AV) extracts and investigate their respective antioxidant activities. Herein, an ultra-high pressure liquid chromatography-mass spectrometer (UPLC-MS/MS) analytical method has been developed for the separation, and systematic characterization of the phenolic compounds in HS, CA and AV extracts and was compared along with ten standard phenolic compounds. Additionally, in vitro antioxidant activity of the phenolic compounds was also determined. The HS extract revealed excellent antioxidant activity such as 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity (IC50=19.7 ± 1.2 μg/mL), total reduction capability (0.169 ± 0.003 at 100 μg/mL), nitric oxide radical scavenging activity (IC50=39.33 ± 3.2 μg/mL), metal chelating activity (IC50=56.51 ± 3.6 μg/mL) and inhibition of lipid peroxidation (IC50=12.34 ± 2.3 μg/mL) as compared to CA and AV extracts. Furthermore, catechin, epicatechin, quercetin and chlorogenic acid were found to be the major components responsible for the antioxidant activity of the HS extract as evidenced from UPLC-MS/MS. Taken together, this study demonstrates the promising antioxidant properties of the HS extract, which can further be utilized in various pharmaceutical, food, and agricultural applications. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/26948646/Rapid_screening_and_identification_of_phenolic_antioxidants_in_Hydrocotyle_sibthorpioides_Lam__by_UPLC_ESI_MS/MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(16)30262-X DB - PRIME DP - Unbound Medicine ER -