Tags

Type your tag names separated by a space and hit enter

Toxic and genotoxic effects of the 2,4-dichlorophenoxyacetic acid (2,4-D)-based herbicide on the Neotropical fish Cnesterodon decemmaculatus.
Ecotoxicol Environ Saf. 2016 Jun; 128:222-9.EE

Abstract

Acute toxicity and genotoxicity of the 54.8% 2,4-D-based commercial herbicide DMA® were assayed on Cnesterodon decemmaculatus (Pisces, Poeciliidae). Whereas lethal effect was used as the end point for mortality, frequency of micronuclei (MNs), other nuclear abnormalities and primary DNA damage evaluated by the single cell gel electrophoresis (SCGE) assay were employed as end points for genotoxicity. Mortality studies demonstrated an LC50 96 h value of 1008 mg/L (range, 929-1070) of 2,4-D. Behavioral changes, e.g., gathering at the bottom of the aquarium, slowness in motion, slow reaction and abnormal swimming were observed. Exposure to 2,4-D within the 252-756 mg/L range increased the frequency of MNs in fish exposed for both 48 and 96 h. Whereas blebbed nuclei were induced in treatments lasting for 48 and 96 h, notched nuclei were only induced in fish exposed for 96 h. Regardless of both concentration and exposure time, 2,4-D did not induce lobed nuclei and binucleated erythrocytes. In addition, we found that exposure to 2,4-D within the 252-756 mg/L range increased the genetic damage index in treatments lasting for either 48 and 96 h. The results represent the first experimental evidence of the lethal and several sublethal effects, including behavioral alterations and two genotoxic properties namely the induction of MNs and primary DNA strand breaks, exerted by 2,4-D on an endemic organism as C. decemmaculatus.

Authors+Show Affiliations

Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nº 3, B1904AMA, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nº 3, B1904AMA, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nº 3, B1904AMA, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address: marcelo.larramendy@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26950899

Citation

Ruiz de Arcaute, C, et al. "Toxic and Genotoxic Effects of the 2,4-dichlorophenoxyacetic Acid (2,4-D)-based Herbicide On the Neotropical Fish Cnesterodon Decemmaculatus." Ecotoxicology and Environmental Safety, vol. 128, 2016, pp. 222-9.
Ruiz de Arcaute C, Soloneski S, Larramendy ML. Toxic and genotoxic effects of the 2,4-dichlorophenoxyacetic acid (2,4-D)-based herbicide on the Neotropical fish Cnesterodon decemmaculatus. Ecotoxicol Environ Saf. 2016;128:222-9.
Ruiz de Arcaute, C., Soloneski, S., & Larramendy, M. L. (2016). Toxic and genotoxic effects of the 2,4-dichlorophenoxyacetic acid (2,4-D)-based herbicide on the Neotropical fish Cnesterodon decemmaculatus. Ecotoxicology and Environmental Safety, 128, 222-9. https://doi.org/10.1016/j.ecoenv.2016.02.027
Ruiz de Arcaute C, Soloneski S, Larramendy ML. Toxic and Genotoxic Effects of the 2,4-dichlorophenoxyacetic Acid (2,4-D)-based Herbicide On the Neotropical Fish Cnesterodon Decemmaculatus. Ecotoxicol Environ Saf. 2016;128:222-9. PubMed PMID: 26950899.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toxic and genotoxic effects of the 2,4-dichlorophenoxyacetic acid (2,4-D)-based herbicide on the Neotropical fish Cnesterodon decemmaculatus. AU - Ruiz de Arcaute,C, AU - Soloneski,S, AU - Larramendy,M L, Y1 - 2016/03/04/ PY - 2015/12/01/received PY - 2016/02/24/revised PY - 2016/02/25/accepted PY - 2016/3/8/entrez PY - 2016/3/8/pubmed PY - 2016/11/5/medline KW - Agrochemicals KW - Behavioral changes KW - Lethality KW - Micronuclei KW - SCGE assay KW - Sublethal effects SP - 222 EP - 9 JF - Ecotoxicology and environmental safety JO - Ecotoxicol. Environ. Saf. VL - 128 N2 - Acute toxicity and genotoxicity of the 54.8% 2,4-D-based commercial herbicide DMA® were assayed on Cnesterodon decemmaculatus (Pisces, Poeciliidae). Whereas lethal effect was used as the end point for mortality, frequency of micronuclei (MNs), other nuclear abnormalities and primary DNA damage evaluated by the single cell gel electrophoresis (SCGE) assay were employed as end points for genotoxicity. Mortality studies demonstrated an LC50 96 h value of 1008 mg/L (range, 929-1070) of 2,4-D. Behavioral changes, e.g., gathering at the bottom of the aquarium, slowness in motion, slow reaction and abnormal swimming were observed. Exposure to 2,4-D within the 252-756 mg/L range increased the frequency of MNs in fish exposed for both 48 and 96 h. Whereas blebbed nuclei were induced in treatments lasting for 48 and 96 h, notched nuclei were only induced in fish exposed for 96 h. Regardless of both concentration and exposure time, 2,4-D did not induce lobed nuclei and binucleated erythrocytes. In addition, we found that exposure to 2,4-D within the 252-756 mg/L range increased the genetic damage index in treatments lasting for either 48 and 96 h. The results represent the first experimental evidence of the lethal and several sublethal effects, including behavioral alterations and two genotoxic properties namely the induction of MNs and primary DNA strand breaks, exerted by 2,4-D on an endemic organism as C. decemmaculatus. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/26950899/Toxic_and_genotoxic_effects_of_the_24_dichlorophenoxyacetic_acid__24_D__based_herbicide_on_the_Neotropical_fish_Cnesterodon_decemmaculatus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(16)30057-4 DB - PRIME DP - Unbound Medicine ER -