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Comparison of antibody assays for detection of autoantibodies to Ro 52, Ro 60 and La associated with primary Sjögren's syndrome.
J Immunol Methods. 2016 06; 433:44-50.JI

Abstract

Anti-Ro(52/60) and anti-La constitute the hallmark autoantibodies in primary Sjögren's syndrome, being present in 40-70% of sera. Several anti-Ro/La assays exist, but antibody detection appears to be assay-specific, thus the aim of this study was to compare several anti-Ro/La assays. In total, 96 sera from individuals with primary Sjögren's syndrome and 114 healthy controls were tested for anti-Ro 52/60 and anti-La in 17 immunoassays. Especially the immunoassays used for detection of anti-Ro 52 differed in their sensitivity (48-79%), while only small differences in sensitivities were observed for the anti-Ro 60 (69-77%) anti-La (39-44%) assays. Concordances of 65%, 79% and 73% for the anti-Ro 52, anti-Ro 60 and anti-La assays were found, respectively. The majority of the assays yielded high specificities, primarily ranging from 97 to 100%, except from a single anti-Ro 60 assay, which yielded a specificity of 79%. Occasionally, reactivity levels were increased in a few assays, indicating that false-positive results can be obtained when applying assays of reduced specificity. In general, the commercial assays appeared to perform better than the in-house analyses. When correcting the in-house assays for background reactivity, sensitivities were reduced by approximately 7%, 17%, and 19% for anti-Ro 52, anti-Ro 60 and anti-La assays, respectively, illustrating the pitfalls when applying immunoassays for detection of autoantibodies, which in theory may apply to commercial assays as well. Finally, increased total sensitivities were obtained when combining assays. These studies contribute to clarify the clinical utility of immunoassays for detection of autoantibodies of Ro 52, Ro 60 and La and illustrate that the most efficient strategy to maximize antibody sensitivity is to combine several assays.

Authors+Show Affiliations

Statens Serum Institut, Department of Autoimmunology and Biomarkers, Artillerivej 5, DK-2300 Copenhagen, Denmark. Electronic address: nhp@ssi.dk.Statens Serum Institut, Department of Autoimmunology and Biomarkers, Artillerivej 5, DK-2300 Copenhagen, Denmark.Statens Serum Institut, Department of Autoimmunology and Biomarkers, Artillerivej 5, DK-2300 Copenhagen, Denmark.Statens Serum Institut, Department of Autoimmunology and Biomarkers, Artillerivej 5, DK-2300 Copenhagen, Denmark.Department of Rheumatology, Skåne University Hospital, Lund University, S-20502 Malmö, Sweden. Electronic address: elke.theander@med.lu.se.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

26956184

Citation

Trier, Nicole Hartwig, et al. "Comparison of Antibody Assays for Detection of Autoantibodies to Ro 52, Ro 60 and La Associated With Primary Sjögren's Syndrome." Journal of Immunological Methods, vol. 433, 2016, pp. 44-50.
Trier NH, Nielsen IØ, Friis T, et al. Comparison of antibody assays for detection of autoantibodies to Ro 52, Ro 60 and La associated with primary Sjögren's syndrome. J Immunol Methods. 2016;433:44-50.
Trier, N. H., Nielsen, I. Ø., Friis, T., Houen, G., & Theander, E. (2016). Comparison of antibody assays for detection of autoantibodies to Ro 52, Ro 60 and La associated with primary Sjögren's syndrome. Journal of Immunological Methods, 433, 44-50. https://doi.org/10.1016/j.jim.2016.03.001
Trier NH, et al. Comparison of Antibody Assays for Detection of Autoantibodies to Ro 52, Ro 60 and La Associated With Primary Sjögren's Syndrome. J Immunol Methods. 2016;433:44-50. PubMed PMID: 26956184.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of antibody assays for detection of autoantibodies to Ro 52, Ro 60 and La associated with primary Sjögren's syndrome. AU - Trier,Nicole Hartwig, AU - Nielsen,Inger Ødum, AU - Friis,Tina, AU - Houen,Gunnar, AU - Theander,Elke, Y1 - 2016/03/06/ PY - 2016/02/11/received PY - 2016/03/02/revised PY - 2016/03/02/accepted PY - 2016/3/10/entrez PY - 2016/3/10/pubmed PY - 2017/6/24/medline KW - Autoantibodies KW - La KW - Non-specific reactivity KW - Primary Sjögren's syndrome KW - Ro KW - SSA/SSB SP - 44 EP - 50 JF - Journal of immunological methods JO - J. Immunol. Methods VL - 433 N2 - Anti-Ro(52/60) and anti-La constitute the hallmark autoantibodies in primary Sjögren's syndrome, being present in 40-70% of sera. Several anti-Ro/La assays exist, but antibody detection appears to be assay-specific, thus the aim of this study was to compare several anti-Ro/La assays. In total, 96 sera from individuals with primary Sjögren's syndrome and 114 healthy controls were tested for anti-Ro 52/60 and anti-La in 17 immunoassays. Especially the immunoassays used for detection of anti-Ro 52 differed in their sensitivity (48-79%), while only small differences in sensitivities were observed for the anti-Ro 60 (69-77%) anti-La (39-44%) assays. Concordances of 65%, 79% and 73% for the anti-Ro 52, anti-Ro 60 and anti-La assays were found, respectively. The majority of the assays yielded high specificities, primarily ranging from 97 to 100%, except from a single anti-Ro 60 assay, which yielded a specificity of 79%. Occasionally, reactivity levels were increased in a few assays, indicating that false-positive results can be obtained when applying assays of reduced specificity. In general, the commercial assays appeared to perform better than the in-house analyses. When correcting the in-house assays for background reactivity, sensitivities were reduced by approximately 7%, 17%, and 19% for anti-Ro 52, anti-Ro 60 and anti-La assays, respectively, illustrating the pitfalls when applying immunoassays for detection of autoantibodies, which in theory may apply to commercial assays as well. Finally, increased total sensitivities were obtained when combining assays. These studies contribute to clarify the clinical utility of immunoassays for detection of autoantibodies of Ro 52, Ro 60 and La and illustrate that the most efficient strategy to maximize antibody sensitivity is to combine several assays. SN - 1872-7905 UR - https://www.unboundmedicine.com/medline/citation/26956184/Comparison_of_antibody_assays_for_detection_of_autoantibodies_to_Ro_52_Ro_60_and_La_associated_with_primary_Sjögren's_syndrome_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-1759(16)30039-4 DB - PRIME DP - Unbound Medicine ER -