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[Association of SNP of leukocyte differentiation antigen-CD40 gene and its serum level with ischemic stroke].
Zhonghua Yu Fang Yi Xue Za Zhi. 2016 Mar; 50(3):239-43.ZY

Abstract

OBJECTIVE

To investigate the association of SNP of CD40 gene and its serum levels with ischemic stroke (IS).

METHODS

A total of 202 IS patients from a hospital of Baise city were enrolled in case group from May 2013 to November 2014. At the same time, 109 healthy people who had physical check-ups in the outpatient department at the same hospital were enrolled in the control group. All participants were from Guangxi Zhuang Autonomous Region and unrelated to each other. 3 ml venous blood were collected on the premise of informed consent. The single nucleotide polymorphisms of CD40 gene rs1883832 C/T, rs13040307 C/T, rs752118 C/T and rs3765459 G/A were analyzed using a Snapshot SNP genotyping assays, and the serum levels of CD40 were tested by ELISA. t-test was used to compare the serum levels of CD40 between the case and control group, and the genotypes at different locuses in case group; χ(2) test was used to compare the distribution differences of the CD40 gene locuses in different genotypes and allele between the case group and the control group; alleles was established as independent variables, the occurrence of the IS as dependent variable, and expressed relative risk with OR (95%CI) value.

RESULTS

In the case group, the frequency of CC, CT and TT genotypes in CD40 gene rs1883832 C/T were 21.78% (44/202), 49.51% (100/202) and 28.71% (58/202), respectively, and 33.17% (66/199), 48.74% (97/199), 18.09% (36/199) in the control group, respectively, the differences between the two groups was significant (χ(2)=9.57, P=0.008). The CD40 serum levels were (62.7 ± 24.5) pg/ml in the case group, which was higher than that in the control group (45.3 ± 17.2) pg/ml (t=8.97, P<0.001). The serum levels of TT and CT genotypes in CD40 gene were (65.9 ± 26.3) and (64.3 ± 25.9) pg/ml, respectively, and the differences were significant when comparing with CC genotype (t equaled 5.34 and 5.03, respectively, P<0.001). The risk of developing IS was 1.56 times higher in 1883832 T allele carriers than that in rs1883832 C allele carriers (OR=1.56, 95% CI: 1.18-2.06); Combined genotype analysis displayed that CD40 gene rs1883832 C/T, rs13040307 C/T, rs752118 C/T and rs3765459 G/A polymorphisms showed strong linkage disequilibrium, the case group TCCA haplotype was tested to be associated with a significantly increased risk of IS as compared with that in the control group(OR=2.49; 95%CI: 1.13-5.48).

CONCLUSION

CD40 gene rs1883832 C/T polymorphism and its TCCA haplotype were possibly associated with ischemic stroke, and the susceptibility gene for ischemic stroke may be rs1883832 T allele.

Authors+Show Affiliations

Department of Laboratory Medicine, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26957242

Citation

Chen, Jianming, et al. "[Association of SNP of Leukocyte Differentiation antigen-CD40 Gene and Its Serum Level With Ischemic Stroke]." Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine], vol. 50, no. 3, 2016, pp. 239-43.
Chen J, Huang H, Yuan Q, et al. [Association of SNP of leukocyte differentiation antigen-CD40 gene and its serum level with ischemic stroke]. Zhonghua Yu Fang Yi Xue Za Zhi. 2016;50(3):239-43.
Chen, J., Huang, H., Yuan, Q., Luo, H., Xiang, Y., Wang, C., Meng, L., & Wei, Y. (2016). [Association of SNP of leukocyte differentiation antigen-CD40 gene and its serum level with ischemic stroke]. Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine], 50(3), 239-43. https://doi.org/10.3760/cma.j.issn.0253-9624.2016.03.010
Chen J, et al. [Association of SNP of Leukocyte Differentiation antigen-CD40 Gene and Its Serum Level With Ischemic Stroke]. Zhonghua Yu Fang Yi Xue Za Zhi. 2016;50(3):239-43. PubMed PMID: 26957242.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Association of SNP of leukocyte differentiation antigen-CD40 gene and its serum level with ischemic stroke]. AU - Chen,Jianming, AU - Huang,Huatuo, AU - Yuan,Qiuran, AU - Luo,Hongcheng, AU - Xiang,Yang, AU - Wang,Chunfang, AU - Meng,Lanqing, AU - Wei,Yesheng, PY - 2016/3/10/entrez PY - 2016/3/10/pubmed PY - 2016/9/16/medline SP - 239 EP - 43 JF - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] JO - Zhonghua Yu Fang Yi Xue Za Zhi VL - 50 IS - 3 N2 - OBJECTIVE: To investigate the association of SNP of CD40 gene and its serum levels with ischemic stroke (IS). METHODS: A total of 202 IS patients from a hospital of Baise city were enrolled in case group from May 2013 to November 2014. At the same time, 109 healthy people who had physical check-ups in the outpatient department at the same hospital were enrolled in the control group. All participants were from Guangxi Zhuang Autonomous Region and unrelated to each other. 3 ml venous blood were collected on the premise of informed consent. The single nucleotide polymorphisms of CD40 gene rs1883832 C/T, rs13040307 C/T, rs752118 C/T and rs3765459 G/A were analyzed using a Snapshot SNP genotyping assays, and the serum levels of CD40 were tested by ELISA. t-test was used to compare the serum levels of CD40 between the case and control group, and the genotypes at different locuses in case group; χ(2) test was used to compare the distribution differences of the CD40 gene locuses in different genotypes and allele between the case group and the control group; alleles was established as independent variables, the occurrence of the IS as dependent variable, and expressed relative risk with OR (95%CI) value. RESULTS: In the case group, the frequency of CC, CT and TT genotypes in CD40 gene rs1883832 C/T were 21.78% (44/202), 49.51% (100/202) and 28.71% (58/202), respectively, and 33.17% (66/199), 48.74% (97/199), 18.09% (36/199) in the control group, respectively, the differences between the two groups was significant (χ(2)=9.57, P=0.008). The CD40 serum levels were (62.7 ± 24.5) pg/ml in the case group, which was higher than that in the control group (45.3 ± 17.2) pg/ml (t=8.97, P<0.001). The serum levels of TT and CT genotypes in CD40 gene were (65.9 ± 26.3) and (64.3 ± 25.9) pg/ml, respectively, and the differences were significant when comparing with CC genotype (t equaled 5.34 and 5.03, respectively, P<0.001). The risk of developing IS was 1.56 times higher in 1883832 T allele carriers than that in rs1883832 C allele carriers (OR=1.56, 95% CI: 1.18-2.06); Combined genotype analysis displayed that CD40 gene rs1883832 C/T, rs13040307 C/T, rs752118 C/T and rs3765459 G/A polymorphisms showed strong linkage disequilibrium, the case group TCCA haplotype was tested to be associated with a significantly increased risk of IS as compared with that in the control group(OR=2.49; 95%CI: 1.13-5.48). CONCLUSION: CD40 gene rs1883832 C/T polymorphism and its TCCA haplotype were possibly associated with ischemic stroke, and the susceptibility gene for ischemic stroke may be rs1883832 T allele. SN - 0253-9624 UR - https://www.unboundmedicine.com/medline/citation/26957242/[Association_of_SNP_of_leukocyte_differentiation_antigen_CD40_gene_and_its_serum_level_with_ischemic_stroke]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0253-9624&amp;year=2016&amp;vol=50&amp;issue=3&amp;fpage=239 DB - PRIME DP - Unbound Medicine ER -