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Stability of ColE1-like and pBR322-like plasmids in Escherichia coli.
Mol Microbiol. 1989 Dec; 3(12):1745-52.MM

Abstract

The average copy number, the level of ampicillin resistance conferred by one plasmid, and the degree of plasmid multimerization were determined for several ColE1-like and pBR322-like plasmids. From the results obtained, the variance of the units of partition corresponding to each plasmid studied was calculated. Experimentally determined plasmid stability was compared with that calculated using the variance of the units of partition and the ratio between the generation times of plasmid-free and of plasmid-carrying cells, assuming that the units of partition are distributed randomly between daughter cells. Stability of the pBR322-like plasmids present mainly as monomers in the bacterial host was consistent with random partitioning, whereas pBR322-like plasmids, present mainly as dimers, and the ColE1-like plasmid showed greater stability than that predicted with random partitioning at cell division.

Authors+Show Affiliations

Departamento de Biología Molecular, Universidad Nacional Autónoma de México, D.F.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2695746

Citation

Ayala-Sanmartín, J, and M C. Gómez-Eichelmann. "Stability of ColE1-like and pBR322-like Plasmids in Escherichia Coli." Molecular Microbiology, vol. 3, no. 12, 1989, pp. 1745-52.
Ayala-Sanmartín J, Gómez-Eichelmann MC. Stability of ColE1-like and pBR322-like plasmids in Escherichia coli. Mol Microbiol. 1989;3(12):1745-52.
Ayala-Sanmartín, J., & Gómez-Eichelmann, M. C. (1989). Stability of ColE1-like and pBR322-like plasmids in Escherichia coli. Molecular Microbiology, 3(12), 1745-52.
Ayala-Sanmartín J, Gómez-Eichelmann MC. Stability of ColE1-like and pBR322-like Plasmids in Escherichia Coli. Mol Microbiol. 1989;3(12):1745-52. PubMed PMID: 2695746.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stability of ColE1-like and pBR322-like plasmids in Escherichia coli. AU - Ayala-Sanmartín,J, AU - Gómez-Eichelmann,M C, PY - 1989/12/1/pubmed PY - 1989/12/1/medline PY - 1989/12/1/entrez SP - 1745 EP - 52 JF - Molecular microbiology JO - Mol. Microbiol. VL - 3 IS - 12 N2 - The average copy number, the level of ampicillin resistance conferred by one plasmid, and the degree of plasmid multimerization were determined for several ColE1-like and pBR322-like plasmids. From the results obtained, the variance of the units of partition corresponding to each plasmid studied was calculated. Experimentally determined plasmid stability was compared with that calculated using the variance of the units of partition and the ratio between the generation times of plasmid-free and of plasmid-carrying cells, assuming that the units of partition are distributed randomly between daughter cells. Stability of the pBR322-like plasmids present mainly as monomers in the bacterial host was consistent with random partitioning, whereas pBR322-like plasmids, present mainly as dimers, and the ColE1-like plasmid showed greater stability than that predicted with random partitioning at cell division. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/2695746/Stability_of_ColE1_like_and_pBR322_like_plasmids_in_Escherichia_coli_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=1989&volume=3&issue=12&spage=1745 DB - PRIME DP - Unbound Medicine ER -