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Olive Recombinant Hydroperoxide Lyase, an Efficient Biocatalyst for Synthesis of Green Leaf Volatiles.
Appl Biochem Biotechnol. 2016 Jun; 179(4):671-83.AB

Abstract

Volatile C6-aldehydes are the main contributors to the characteristic odor of plants known as "green note" and are widely used by the flavor industry. Biotechnological processes were developed to fulfill the high demand in C6-aldehydes in natural flavorants and odorants. Recombinant hydroperoxide lyases (HPLs) constitute an interesting alternative to overcome drawbacks arising from the use of HPL from plant extracts. Thus, olive recombinant 13-HPL was assayed as biocatalysts to produce C6-aldehydes. Firstly, a cDNA encoding for olive HPL of Leccino variety was isolated and cloned in pQE-30 expression vector. In order to improve the enzyme solubility, its chloroplast transit peptide was deleted. Both enzymes (HPL wild type and HPL deleted) were expressed into Escherichia coli strain M15, purified, characterized, and then used for bioconversion of 13-hydroperoxides of linoleic and linolenic acids. Aldehydes produced were extracted, then identified and quantified using gas chromatography and mass spectrometry. Recombinant HPL wild type (HPLwt) allowed producing 5.61 mM of hexanal and 4.39 mM of 3Z-hexenal, corresponding to high conversion yields of 93.5 and 73 %, respectively. Using HPL deleted (HPLdel) instead of HPLwt failed to obtain greater quantities of hexanal or 3Z-hexenal. No undesirable products were formed, and no isomerization of 3Z-hexenal in 2E-hexenal occurred. The olive recombinant HPLwt appears to be a promising efficient biocatalyst for the production of C6-aldehydes.

Authors+Show Affiliations

Laboratoire de Biochimie et Biologie Moléculaire Végétales, CNRS UMR6134 SPE, Université de Corse, Campus Grimaldi, BP52, 20250, Corte, France.Laboratoire de Biochimie et Biologie Moléculaire Végétales, CNRS UMR6134 SPE, Université de Corse, Campus Grimaldi, BP52, 20250, Corte, France.Laboratoire de Biochimie et Biologie Moléculaire Végétales, CNRS UMR6134 SPE, Université de Corse, Campus Grimaldi, BP52, 20250, Corte, France.Laboratoire de Biochimie et Biologie Moléculaire Végétales, CNRS UMR6134 SPE, Université de Corse, Campus Grimaldi, BP52, 20250, Corte, France.Laboratoire de Biochimie et Biologie Moléculaire Végétales, CNRS UMR6134 SPE, Université de Corse, Campus Grimaldi, BP52, 20250, Corte, France.Laboratoire de Chimie des Produits Naturels, CNRS UMR6134 SPE, Université de Corse, Campus Grimaldi, BP52, 20250, Corte, France.Laboratoire de Chimie des Produits Naturels, CNRS UMR6134 SPE, Université de Corse, Campus Grimaldi, BP52, 20250, Corte, France.Laboratoire de Chimie des Produits Naturels, CNRS UMR6134 SPE, Université de Corse, Campus Grimaldi, BP52, 20250, Corte, France.Laboratoire de Biochimie et Biologie Moléculaire Végétales, CNRS UMR6134 SPE, Université de Corse, Campus Grimaldi, BP52, 20250, Corte, France.Laboratoire de Biochimie et Biologie Moléculaire Végétales, CNRS UMR6134 SPE, Université de Corse, Campus Grimaldi, BP52, 20250, Corte, France. maury@univ-corse.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26961190

Citation

Jacopini, Sabrina, et al. "Olive Recombinant Hydroperoxide Lyase, an Efficient Biocatalyst for Synthesis of Green Leaf Volatiles." Applied Biochemistry and Biotechnology, vol. 179, no. 4, 2016, pp. 671-83.
Jacopini S, Mariani M, de Caraffa VB, et al. Olive Recombinant Hydroperoxide Lyase, an Efficient Biocatalyst for Synthesis of Green Leaf Volatiles. Appl Biochem Biotechnol. 2016;179(4):671-83.
Jacopini, S., Mariani, M., de Caraffa, V. B., Gambotti, C., Vincenti, S., Desjobert, J. M., Muselli, A., Costa, J., Berti, L., & Maury, J. (2016). Olive Recombinant Hydroperoxide Lyase, an Efficient Biocatalyst for Synthesis of Green Leaf Volatiles. Applied Biochemistry and Biotechnology, 179(4), 671-83. https://doi.org/10.1007/s12010-016-2023-x
Jacopini S, et al. Olive Recombinant Hydroperoxide Lyase, an Efficient Biocatalyst for Synthesis of Green Leaf Volatiles. Appl Biochem Biotechnol. 2016;179(4):671-83. PubMed PMID: 26961190.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Olive Recombinant Hydroperoxide Lyase, an Efficient Biocatalyst for Synthesis of Green Leaf Volatiles. AU - Jacopini,Sabrina, AU - Mariani,Magali, AU - de Caraffa,Virginie Brunini-Bronzini, AU - Gambotti,Claude, AU - Vincenti,Sophie, AU - Desjobert,Jean-Marie, AU - Muselli,Alain, AU - Costa,Jean, AU - Berti,Liliane, AU - Maury,Jacques, Y1 - 2016/03/10/ PY - 2015/10/10/received PY - 2016/02/18/accepted PY - 2016/3/11/entrez PY - 2016/3/11/pubmed PY - 2017/2/1/medline KW - 3Z-Hexenal KW - Bioconversion KW - C6-aldehydes KW - Green note KW - Hexanal KW - Hydroperoxide lyase SP - 671 EP - 83 JF - Applied biochemistry and biotechnology JO - Appl Biochem Biotechnol VL - 179 IS - 4 N2 - Volatile C6-aldehydes are the main contributors to the characteristic odor of plants known as "green note" and are widely used by the flavor industry. Biotechnological processes were developed to fulfill the high demand in C6-aldehydes in natural flavorants and odorants. Recombinant hydroperoxide lyases (HPLs) constitute an interesting alternative to overcome drawbacks arising from the use of HPL from plant extracts. Thus, olive recombinant 13-HPL was assayed as biocatalysts to produce C6-aldehydes. Firstly, a cDNA encoding for olive HPL of Leccino variety was isolated and cloned in pQE-30 expression vector. In order to improve the enzyme solubility, its chloroplast transit peptide was deleted. Both enzymes (HPL wild type and HPL deleted) were expressed into Escherichia coli strain M15, purified, characterized, and then used for bioconversion of 13-hydroperoxides of linoleic and linolenic acids. Aldehydes produced were extracted, then identified and quantified using gas chromatography and mass spectrometry. Recombinant HPL wild type (HPLwt) allowed producing 5.61 mM of hexanal and 4.39 mM of 3Z-hexenal, corresponding to high conversion yields of 93.5 and 73 %, respectively. Using HPL deleted (HPLdel) instead of HPLwt failed to obtain greater quantities of hexanal or 3Z-hexenal. No undesirable products were formed, and no isomerization of 3Z-hexenal in 2E-hexenal occurred. The olive recombinant HPLwt appears to be a promising efficient biocatalyst for the production of C6-aldehydes. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/26961190/Olive_Recombinant_Hydroperoxide_Lyase_an_Efficient_Biocatalyst_for_Synthesis_of_Green_Leaf_Volatiles_ L2 - https://dx.doi.org/10.1007/s12010-016-2023-x DB - PRIME DP - Unbound Medicine ER -