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Characterization of wheat - Psathyrostachys huashanica small segment translocation line with enhanced kernels per spike and stripe rust resistance.
Genome. 2016 Apr; 59(4):221-9.G

Abstract

Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs), a distant wild relative of common wheat, possesses rich potentially valuable traits, such as disease resistance and more spikelets and kernels per spike, that could be useful for wheat genetic improvement. Development of wheat - P. huashanica translocation lines will facilitate its practical utilization in wheat breeding. In the present study, a wheat - P. huashanica small segmental translocation line, K-13-835-3, was isolated and characterized from the BC1F5 population of a cross between wheat - P. huashanica amphiploid PHW-SA and wheat cultivar CN16. Cytological studies showed that the mean chromosome configuration of K-13-835-3 at meiosis was 2n = 42 = 0.10 I + 19.43 II (ring) + 1.52 II (rod). GISH analyses indicated that chromosome composition of K-13-835-3 included 40 wheat chromosomes and a pair of wheat - P. huashanica translocation chromosomes. FISH results demonstrated that the small segment from an unidentified P. huashanica chromosome was translocated into wheat chromosome arm 5DS, proximal to the centromere region of 5DS. Compared with the cultivar wheat parent CN16, K-13-835-3 was highly resistant to stripe rust pathogens prevalent in China. Furthermore, spikelets and kernels per spike in K-13-835-3 were significantly higher than those of CN16 in two growing seasons. These results suggest that the desirable genes from P. huashanica were successfully transferred into CN16 background. This translocation line could be used as novel germplasm for high-yield and, eventually, resistant cultivar breeding.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kang HY
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
Zhang ZJ
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
Xu LL
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
Qi WL
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
Tang Y
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
Wang H
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
Zhu W
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
Li DY
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
Zeng J
b College of Resources, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
Wang Y
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
Fan X
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
Sha LN
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
Zhang HQ
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
Zhou YH
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.

MeSH

BasidiomycotaChromosomes, PlantDisease ResistanceHybridization, GeneticPhenotypePlant BreedingPlant DiseasesPoaceaeTranslocation, GeneticTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26961208

Citation

Kang, Hou-Yang, et al. "Characterization of Wheat - Psathyrostachys Huashanica Small Segment Translocation Line With Enhanced Kernels Per Spike and Stripe Rust Resistance." Genome, vol. 59, no. 4, 2016, pp. 221-9.
Kang HY, Zhang ZJ, Xu LL, et al. Characterization of wheat - Psathyrostachys huashanica small segment translocation line with enhanced kernels per spike and stripe rust resistance. Genome. 2016;59(4):221-9.
Kang, H. Y., Zhang, Z. J., Xu, L. L., Qi, W. L., Tang, Y., Wang, H., Zhu, W., Li, D. Y., Zeng, J., Wang, Y., Fan, X., Sha, L. N., Zhang, H. Q., & Zhou, Y. H. (2016). Characterization of wheat - Psathyrostachys huashanica small segment translocation line with enhanced kernels per spike and stripe rust resistance. Genome, 59(4), 221-9. https://doi.org/10.1139/gen-2015-0138
Kang HY, et al. Characterization of Wheat - Psathyrostachys Huashanica Small Segment Translocation Line With Enhanced Kernels Per Spike and Stripe Rust Resistance. Genome. 2016;59(4):221-9. PubMed PMID: 26961208.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of wheat - Psathyrostachys huashanica small segment translocation line with enhanced kernels per spike and stripe rust resistance. AU - Kang,Hou-Yang, AU - Zhang,Zhi-Juan, AU - Xu,Li-Li, AU - Qi,Wei-Liang, AU - Tang,Yao, AU - Wang,Hao, AU - Zhu,Wei, AU - Li,Dai-Yan, AU - Zeng,Jian, AU - Wang,Yi, AU - Fan,Xing, AU - Sha,Li-Na, AU - Zhang,Hai-Qin, AU - Zhou,Yong-Hong, Y1 - 2016/02/02/ PY - 2016/3/11/entrez PY - 2016/3/11/pubmed PY - 2016/11/15/medline KW - Psathyrostachys huashanica KW - kernels per spike KW - lignée à translocation KW - nombre de grains pas épi KW - rouille jaune KW - stripe rust KW - translocation line SP - 221 EP - 9 JF - Genome JO - Genome VL - 59 IS - 4 N2 - Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs), a distant wild relative of common wheat, possesses rich potentially valuable traits, such as disease resistance and more spikelets and kernels per spike, that could be useful for wheat genetic improvement. Development of wheat - P. huashanica translocation lines will facilitate its practical utilization in wheat breeding. In the present study, a wheat - P. huashanica small segmental translocation line, K-13-835-3, was isolated and characterized from the BC1F5 population of a cross between wheat - P. huashanica amphiploid PHW-SA and wheat cultivar CN16. Cytological studies showed that the mean chromosome configuration of K-13-835-3 at meiosis was 2n = 42 = 0.10 I + 19.43 II (ring) + 1.52 II (rod). GISH analyses indicated that chromosome composition of K-13-835-3 included 40 wheat chromosomes and a pair of wheat - P. huashanica translocation chromosomes. FISH results demonstrated that the small segment from an unidentified P. huashanica chromosome was translocated into wheat chromosome arm 5DS, proximal to the centromere region of 5DS. Compared with the cultivar wheat parent CN16, K-13-835-3 was highly resistant to stripe rust pathogens prevalent in China. Furthermore, spikelets and kernels per spike in K-13-835-3 were significantly higher than those of CN16 in two growing seasons. These results suggest that the desirable genes from P. huashanica were successfully transferred into CN16 background. This translocation line could be used as novel germplasm for high-yield and, eventually, resistant cultivar breeding. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/26961208/Characterization_of_wheat___Psathyrostachys_huashanica_small_segment_translocation_line_with_enhanced_kernels_per_spike_and_stripe_rust_resistance_ DB - PRIME DP - Unbound Medicine ER -