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The Effect of GCSB-5 a New Herbal Medicine on Changes in Pain Behavior and Neuroglial Activation in a Rat Model of Lumbar Disc Herniation.
J Korean Neurosurg Soc. 2016 Mar; 59(2):98-105.JK

Abstract

OBJECTIVE

Lumbar disc herniation can induce sciatica by mechanical compression and/or chemical irritation. The aim of this study was to compare the effects of GCSB-5 (Shinbaro®) and NSAIDs on pain-related behavior and on the expressions of microglia, astrocytes, CGRP, TRPV1, IL-6, and CX3CL1 in a rat model of lumbar disc herniation.

METHODS

112 male Sprague-Dawley rats underwent implantation of nucleus pulposus to a dorsal root ganglion (DRG). Rats were divided into five groups as follows; a saline group (the vehicle control group) (n=27), a 10 mg/kg aceclofenac group (the aceclofenac group) (n=22), and 100, 300 or 600 mg/kg GCSB-5 groups (the GCSB-5 100, 300, or 600 groups) (n=21 for each group). Rats were tested for mechanical allodynia at 3 days after surgery and at 1 day, 3 days, 7 days, 14 days, 21 days, 28 days, 35 days, 42 days, 49 days, and 56 days after treatment commencement. Immunohistochemical staining of microglia (Iba1), astrocytes (GFAP), CGRP, and TRPV1, and PCR for IL-6 and CX3CL1 were performed on spinal dorsal horns and DRGs at 56 days after medication commencement.

RESULTS

After 56 days of GCSB-5 300 administration, mechanical withdrawal thresholds were significantly increased (p<0.05), and immunohisto-chemical expressions of Iba1, GFAP, CGRP, and TRPV1 were reduced than other groups, but this difference was not statistically significant.

CONCLUSION

These results indicate GCSB-5 reduces mechanical allodynia and downregulates neuroglial activity and the expressions of CGRP and TRPV1 in the spinal segments of a rat model of lumbar disc herniation.

Authors+Show Affiliations

Department of Physical Medicine and Rehabilitation, College of Medicine, Catholic University of Daegu, Daegu, Korea.Medical Devices Clinical Trial Center, College of Medicine, Yeungnam University, Daegu, Korea.Medical Devices Clinical Trial Center, College of Medicine, Yeungnam University, Daegu, Korea.Department of Physical Medicine and Rehabilitation, College of Medicine, Yeungnam University, Daegu, Korea.Department of Medical Statistics, College of Medicine, Catholic University of Daegu, Daegu, Korea.Medical Devices Clinical Trial Center, College of Medicine, Yeungnam University, Daegu, Korea.; Department of Physical Medicine and Rehabilitation, College of Medicine, Yeungnam University, Daegu, Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26962414

Citation

Cho, Hee Kyung, et al. "The Effect of GCSB-5 a New Herbal Medicine On Changes in Pain Behavior and Neuroglial Activation in a Rat Model of Lumbar Disc Herniation." Journal of Korean Neurosurgical Society, vol. 59, no. 2, 2016, pp. 98-105.
Cho HK, Kim SY, Choi MJ, et al. The Effect of GCSB-5 a New Herbal Medicine on Changes in Pain Behavior and Neuroglial Activation in a Rat Model of Lumbar Disc Herniation. J Korean Neurosurg Soc. 2016;59(2):98-105.
Cho, H. K., Kim, S. Y., Choi, M. J., Baek, S. O., Kwak, S. G., & Ahn, S. H. (2016). The Effect of GCSB-5 a New Herbal Medicine on Changes in Pain Behavior and Neuroglial Activation in a Rat Model of Lumbar Disc Herniation. Journal of Korean Neurosurgical Society, 59(2), 98-105. https://doi.org/10.3340/jkns.2016.59.2.98
Cho HK, et al. The Effect of GCSB-5 a New Herbal Medicine On Changes in Pain Behavior and Neuroglial Activation in a Rat Model of Lumbar Disc Herniation. J Korean Neurosurg Soc. 2016;59(2):98-105. PubMed PMID: 26962414.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Effect of GCSB-5 a New Herbal Medicine on Changes in Pain Behavior and Neuroglial Activation in a Rat Model of Lumbar Disc Herniation. AU - Cho,Hee Kyung, AU - Kim,So-Yeon, AU - Choi,Mi Jung, AU - Baek,Seung Ok, AU - Kwak,Sang Gyu, AU - Ahn,Sang Ho, Y1 - 2016/02/29/ PY - 2015/09/11/received PY - 2015/09/24/revised PY - 2016/02/10/accepted PY - 2016/3/11/entrez PY - 2016/3/11/pubmed PY - 2016/3/11/medline KW - Astrocytes KW - Calcitonin gene-related peptide KW - GCSB-5 KW - Lumbar disc herniation KW - Microglia KW - Neuropathic pain SP - 98 EP - 105 JF - Journal of Korean Neurosurgical Society JO - J Korean Neurosurg Soc VL - 59 IS - 2 N2 - OBJECTIVE: Lumbar disc herniation can induce sciatica by mechanical compression and/or chemical irritation. The aim of this study was to compare the effects of GCSB-5 (Shinbaro®) and NSAIDs on pain-related behavior and on the expressions of microglia, astrocytes, CGRP, TRPV1, IL-6, and CX3CL1 in a rat model of lumbar disc herniation. METHODS: 112 male Sprague-Dawley rats underwent implantation of nucleus pulposus to a dorsal root ganglion (DRG). Rats were divided into five groups as follows; a saline group (the vehicle control group) (n=27), a 10 mg/kg aceclofenac group (the aceclofenac group) (n=22), and 100, 300 or 600 mg/kg GCSB-5 groups (the GCSB-5 100, 300, or 600 groups) (n=21 for each group). Rats were tested for mechanical allodynia at 3 days after surgery and at 1 day, 3 days, 7 days, 14 days, 21 days, 28 days, 35 days, 42 days, 49 days, and 56 days after treatment commencement. Immunohistochemical staining of microglia (Iba1), astrocytes (GFAP), CGRP, and TRPV1, and PCR for IL-6 and CX3CL1 were performed on spinal dorsal horns and DRGs at 56 days after medication commencement. RESULTS: After 56 days of GCSB-5 300 administration, mechanical withdrawal thresholds were significantly increased (p<0.05), and immunohisto-chemical expressions of Iba1, GFAP, CGRP, and TRPV1 were reduced than other groups, but this difference was not statistically significant. CONCLUSION: These results indicate GCSB-5 reduces mechanical allodynia and downregulates neuroglial activity and the expressions of CGRP and TRPV1 in the spinal segments of a rat model of lumbar disc herniation. SN - 2005-3711 UR - https://www.unboundmedicine.com/medline/citation/26962414/The_Effect_of_GCSB_5_a_New_Herbal_Medicine_on_Changes_in_Pain_Behavior_and_Neuroglial_Activation_in_a_Rat_Model_of_Lumbar_Disc_Herniation_ L2 - http://jkns.or.kr/journal/view.php?doi=10.3340/jkns.2016.59.2.98 DB - PRIME DP - Unbound Medicine ER -
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