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Protein Binding Bends the Gold Nanoparticle Capped DNA Sequence: Toward Novel Energy-Transfer-Based Photoelectrochemical Protein Detection.
Anal Chem. 2016 Apr 05; 88(7):3864-71.AC

Abstract

In this work, we present a novel energy-transfer (ET)-based photoelectrochemical (PEC) probing of DNA-protein interactions, which associates intimately with many important intracellular processes in transcriptional regulatory networks. Specifically, Au nanoparticles (NPs) were confined onto the CdS quantum dots (QDs) functionalized PEC surface by the formation of duplex DNA, the subsequent binding of the TATA binding protein (TBP) and the resulting distortion of the Au NPs capped DNA sequence could adjust the interparticle distance and thereby modulate the PEC performance of CdS QDs through the ET process between the CdS QDs and Au NPs. Using the duplex DNA sequence as a rigid spacer, the relationship between the photocurrent quenching effect and the spacing distance was also studied and some experimental conditions were optimized, on the basis of which a novel ET-based PEC TBP biosensor was realized with high sensitivity and selectivity.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ma ZY
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Ruan YF
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Xu F
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Zhao WW
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Xu JJ
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Chen HY
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.

MeSH

Base SequenceBiosensing TechniquesCadmium CompoundsDNAElectrochemical TechniquesEnergy TransferGoldMetal NanoparticlesPhotochemical ProcessesProtein BindingQuantum DotsSulfidesTATA-Box Binding Protein

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26967949

Citation

Ma, Zheng-Yuan, et al. "Protein Binding Bends the Gold Nanoparticle Capped DNA Sequence: Toward Novel Energy-Transfer-Based Photoelectrochemical Protein Detection." Analytical Chemistry, vol. 88, no. 7, 2016, pp. 3864-71.
Ma ZY, Ruan YF, Xu F, et al. Protein Binding Bends the Gold Nanoparticle Capped DNA Sequence: Toward Novel Energy-Transfer-Based Photoelectrochemical Protein Detection. Anal Chem. 2016;88(7):3864-71.
Ma, Z. Y., Ruan, Y. F., Xu, F., Zhao, W. W., Xu, J. J., & Chen, H. Y. (2016). Protein Binding Bends the Gold Nanoparticle Capped DNA Sequence: Toward Novel Energy-Transfer-Based Photoelectrochemical Protein Detection. Analytical Chemistry, 88(7), 3864-71. https://doi.org/10.1021/acs.analchem.6b00012
Ma ZY, et al. Protein Binding Bends the Gold Nanoparticle Capped DNA Sequence: Toward Novel Energy-Transfer-Based Photoelectrochemical Protein Detection. Anal Chem. 2016 Apr 5;88(7):3864-71. PubMed PMID: 26967949.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protein Binding Bends the Gold Nanoparticle Capped DNA Sequence: Toward Novel Energy-Transfer-Based Photoelectrochemical Protein Detection. AU - Ma,Zheng-Yuan, AU - Ruan,Yi-Fan, AU - Xu,Fei, AU - Zhao,Wei-Wei, AU - Xu,Jing-Juan, AU - Chen,Hong-Yuan, Y1 - 2016/03/18/ PY - 2016/3/12/entrez PY - 2016/3/12/pubmed PY - 2016/12/16/medline SP - 3864 EP - 71 JF - Analytical chemistry JO - Anal Chem VL - 88 IS - 7 N2 - In this work, we present a novel energy-transfer (ET)-based photoelectrochemical (PEC) probing of DNA-protein interactions, which associates intimately with many important intracellular processes in transcriptional regulatory networks. Specifically, Au nanoparticles (NPs) were confined onto the CdS quantum dots (QDs) functionalized PEC surface by the formation of duplex DNA, the subsequent binding of the TATA binding protein (TBP) and the resulting distortion of the Au NPs capped DNA sequence could adjust the interparticle distance and thereby modulate the PEC performance of CdS QDs through the ET process between the CdS QDs and Au NPs. Using the duplex DNA sequence as a rigid spacer, the relationship between the photocurrent quenching effect and the spacing distance was also studied and some experimental conditions were optimized, on the basis of which a novel ET-based PEC TBP biosensor was realized with high sensitivity and selectivity. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/26967949/Protein_Binding_Bends_the_Gold_Nanoparticle_Capped_DNA_Sequence:_Toward_Novel_Energy_Transfer_Based_Photoelectrochemical_Protein_Detection_ DB - PRIME DP - Unbound Medicine ER -