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A Functional Bacterium-to-Plant DNA Transfer Machinery of Rhizobium etli.
PLoS Pathog. 2016 Mar; 12(3):e1005502.PP

Abstract

Different strains and species of the soil phytopathogen Agrobacterium possess the ability to transfer and integrate a segment of DNA (T-DNA) into the genome of their eukaryotic hosts, which is mainly mediated by a set of virulence (vir) genes located on the bacterial Ti-plasmid that also contains the T-DNA. To date, Agrobacterium is considered to be unique in its capacity to mediate genetic transformation of eukaryotes. However, close homologs of the vir genes are encoded by the p42a plasmid of Rhizobium etli; this microorganism is related to Agrobacterium, but known only as a symbiotic bacterium that forms nitrogen-fixing nodules in several species of beans. Here, we show that R. etli can mediate functional DNA transfer and stable genetic transformation of plant cells, when provided with a plasmid containing a T-DNA segment. Thus, R. etli represents another bacterial species, besides Agrobacterium, that encodes a protein machinery for DNA transfer to eukaryotic cells and their subsequent genetic modification.

Authors+Show Affiliations

Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York, United States of America.Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York, United States of America.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

26968003

Citation

Lacroix, Benoît, and Vitaly Citovsky. "A Functional Bacterium-to-Plant DNA Transfer Machinery of Rhizobium Etli." PLoS Pathogens, vol. 12, no. 3, 2016, pp. e1005502.
Lacroix B, Citovsky V. A Functional Bacterium-to-Plant DNA Transfer Machinery of Rhizobium etli. PLoS Pathog. 2016;12(3):e1005502.
Lacroix, B., & Citovsky, V. (2016). A Functional Bacterium-to-Plant DNA Transfer Machinery of Rhizobium etli. PLoS Pathogens, 12(3), e1005502. https://doi.org/10.1371/journal.ppat.1005502
Lacroix B, Citovsky V. A Functional Bacterium-to-Plant DNA Transfer Machinery of Rhizobium Etli. PLoS Pathog. 2016;12(3):e1005502. PubMed PMID: 26968003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Functional Bacterium-to-Plant DNA Transfer Machinery of Rhizobium etli. AU - Lacroix,Benoît, AU - Citovsky,Vitaly, Y1 - 2016/03/11/ PY - 2014/08/28/received PY - 2016/02/22/accepted PY - 2016/3/12/entrez PY - 2016/3/12/pubmed PY - 2016/8/27/medline SP - e1005502 EP - e1005502 JF - PLoS pathogens JO - PLoS Pathog. VL - 12 IS - 3 N2 - Different strains and species of the soil phytopathogen Agrobacterium possess the ability to transfer and integrate a segment of DNA (T-DNA) into the genome of their eukaryotic hosts, which is mainly mediated by a set of virulence (vir) genes located on the bacterial Ti-plasmid that also contains the T-DNA. To date, Agrobacterium is considered to be unique in its capacity to mediate genetic transformation of eukaryotes. However, close homologs of the vir genes are encoded by the p42a plasmid of Rhizobium etli; this microorganism is related to Agrobacterium, but known only as a symbiotic bacterium that forms nitrogen-fixing nodules in several species of beans. Here, we show that R. etli can mediate functional DNA transfer and stable genetic transformation of plant cells, when provided with a plasmid containing a T-DNA segment. Thus, R. etli represents another bacterial species, besides Agrobacterium, that encodes a protein machinery for DNA transfer to eukaryotic cells and their subsequent genetic modification. SN - 1553-7374 UR - https://www.unboundmedicine.com/medline/citation/26968003/A_Functional_Bacterium_to_Plant_DNA_Transfer_Machinery_of_Rhizobium_etli_ L2 - http://dx.plos.org/10.1371/journal.ppat.1005502 DB - PRIME DP - Unbound Medicine ER -