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Single analyzer precursor scans using an ion trap.
Rapid Commun Mass Spectrom. 2016 Apr 15; 30(7):800-4.RC

Abstract

RATIONALE

Precursor ion and neutral loss scans are general survey methods of tandem mass spectrometry (MS/MS) used for detecting structurally related compounds. Until now they have been performed in multiple analyzer instruments, e.g. triple quadrupoles and hybrid MS/MS instruments. Implementation of precursor ion scans in single mass analyzers would be advantageous in reducing instrument complexity.

METHODS

Adoption of secular frequency scanning as a method of mass-selective excitation is shown to enable precursor scans in a single ion trap in a miniature mass spectrometer. A small supplementary alternating current (ac) signal is swept in frequency so as to cause mass-selective excitation of trapped ions. Simultaneously, a higher fixed amplitude ac signal is applied at the fixed secular frequency of a product ion, ejecting the mass-selected product ion and providing temporal data corresponding to a precursor ion spectrum.

RESULTS

Precursor scanning in a single ion trap is demonstrated using a mixture of three illicit drugs: cocaine, 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxymethamphetamine (MDMA). Acquisition of the spectra as a function of the frequency of the product ejection waveform demonstrates that the signals acquired represent precursor ion scans.

CONCLUSIONS

Secular frequency scanning is a nonconventional method of mass scanning that in combination with product ion ejection enables precursor scans in single ion traps. This phenomenon is demonstrated here for a miniature linear ion trap, but the concepts described also apply to quadrupole mass filters. Copyright © 2016 John Wiley & Sons, Ltd.

Authors+Show Affiliations

Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University, West Lafayette, IN, 47907, USA.Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University, West Lafayette, IN, 47907, USA.Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University, West Lafayette, IN, 47907, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26969921

Citation

Snyder, Dalton T., et al. "Single Analyzer Precursor Scans Using an Ion Trap." Rapid Communications in Mass Spectrometry : RCM, vol. 30, no. 7, 2016, pp. 800-4.
Snyder DT, Pulliam CJ, Cooks RG. Single analyzer precursor scans using an ion trap. Rapid Commun Mass Spectrom. 2016;30(7):800-4.
Snyder, D. T., Pulliam, C. J., & Cooks, R. G. (2016). Single analyzer precursor scans using an ion trap. Rapid Communications in Mass Spectrometry : RCM, 30(7), 800-4. https://doi.org/10.1002/rcm.7500
Snyder DT, Pulliam CJ, Cooks RG. Single Analyzer Precursor Scans Using an Ion Trap. Rapid Commun Mass Spectrom. 2016 Apr 15;30(7):800-4. PubMed PMID: 26969921.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single analyzer precursor scans using an ion trap. AU - Snyder,Dalton T, AU - Pulliam,Christopher J, AU - Cooks,R Graham, PY - 2015/11/20/received PY - 2015/12/22/revised PY - 2015/12/23/accepted PY - 2016/3/13/entrez PY - 2016/3/13/pubmed PY - 2016/3/13/medline SP - 800 EP - 4 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun. Mass Spectrom. VL - 30 IS - 7 N2 - RATIONALE: Precursor ion and neutral loss scans are general survey methods of tandem mass spectrometry (MS/MS) used for detecting structurally related compounds. Until now they have been performed in multiple analyzer instruments, e.g. triple quadrupoles and hybrid MS/MS instruments. Implementation of precursor ion scans in single mass analyzers would be advantageous in reducing instrument complexity. METHODS: Adoption of secular frequency scanning as a method of mass-selective excitation is shown to enable precursor scans in a single ion trap in a miniature mass spectrometer. A small supplementary alternating current (ac) signal is swept in frequency so as to cause mass-selective excitation of trapped ions. Simultaneously, a higher fixed amplitude ac signal is applied at the fixed secular frequency of a product ion, ejecting the mass-selected product ion and providing temporal data corresponding to a precursor ion spectrum. RESULTS: Precursor scanning in a single ion trap is demonstrated using a mixture of three illicit drugs: cocaine, 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxymethamphetamine (MDMA). Acquisition of the spectra as a function of the frequency of the product ejection waveform demonstrates that the signals acquired represent precursor ion scans. CONCLUSIONS: Secular frequency scanning is a nonconventional method of mass scanning that in combination with product ion ejection enables precursor scans in single ion traps. This phenomenon is demonstrated here for a miniature linear ion trap, but the concepts described also apply to quadrupole mass filters. Copyright © 2016 John Wiley & Sons, Ltd. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/26969921/Single_analyzer_precursor_scans_using_an_ion_trap_ L2 - https://doi.org/10.1002/rcm.7500 DB - PRIME DP - Unbound Medicine ER -
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