Tags

Type your tag names separated by a space and hit enter

Synergistic dynamics of nitrogen and phosphorous influences lipid productivity in Chlorella minutissima for biodiesel production.
Bioresour Technol. 2016 Aug; 213:79-87.BT

Abstract

The study synergistically optimized nitrogen and phosphorous concentrations for attainment of maximum lipid productivity in Chlorella minutissima. Nitrogen and phosphorous limited cells (N(L)P(L)) showed maximum lipid productivity (49.1±0.41mg/L/d), 1.47 folds higher than control. Nitrogen depletion resulted in reduced cell size with large sized lipid droplets encompassing most of the intracellular space while discrete lipid bodies were observed under nitrogen sufficiency. Synergistic N/P starvations showed more prominent effect on photosynthetic pigments as to individual deprivations. Phosphorous deficiency along with N starvation exhibited 17.12% decline in carbohydrate while no change in nitrogen sufficient cells were recorded. The optimum N(L)P(L) concentration showed balance between biomass and lipid by maintaining intermediate cell size, pigments, carbohydrate and proteins. FAME profile showed C14-C18 carbon chains in N(L)P(L) cells with biodiesel properties comparable to plant oil methyl esters. Hence, synergistic N/P limitation was effective for enhancing lipid productivity with reduced consumption of nutrients.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Arora N
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India.
Patel A
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India.
Pruthi PA
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India.
Pruthi V
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India. Electronic address: vikasfbs@gmail.com.

MeSH

BiofuelsBiomassBiotechnologyCarbohydratesChlorellaEstersLipidsNitrogenPhosphorusPigments, BiologicalProteins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26970694

Citation

Arora, Neha, et al. "Synergistic Dynamics of Nitrogen and Phosphorous Influences Lipid Productivity in Chlorella Minutissima for Biodiesel Production." Bioresource Technology, vol. 213, 2016, pp. 79-87.
Arora N, Patel A, Pruthi PA, et al. Synergistic dynamics of nitrogen and phosphorous influences lipid productivity in Chlorella minutissima for biodiesel production. Bioresour Technol. 2016;213:79-87.
Arora, N., Patel, A., Pruthi, P. A., & Pruthi, V. (2016). Synergistic dynamics of nitrogen and phosphorous influences lipid productivity in Chlorella minutissima for biodiesel production. Bioresource Technology, 213, 79-87. https://doi.org/10.1016/j.biortech.2016.02.112
Arora N, et al. Synergistic Dynamics of Nitrogen and Phosphorous Influences Lipid Productivity in Chlorella Minutissima for Biodiesel Production. Bioresour Technol. 2016;213:79-87. PubMed PMID: 26970694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synergistic dynamics of nitrogen and phosphorous influences lipid productivity in Chlorella minutissima for biodiesel production. AU - Arora,Neha, AU - Patel,Alok, AU - Pruthi,Parul A, AU - Pruthi,Vikas, Y1 - 2016/03/02/ PY - 2015/12/30/received PY - 2016/02/22/revised PY - 2016/02/24/accepted PY - 2016/3/14/entrez PY - 2016/3/14/pubmed PY - 2017/1/24/medline KW - Biodiesel KW - Chlorella minutissima KW - Lipid productivity KW - Nitrogen KW - Phosphorous SP - 79 EP - 87 JF - Bioresource technology JO - Bioresour Technol VL - 213 N2 - The study synergistically optimized nitrogen and phosphorous concentrations for attainment of maximum lipid productivity in Chlorella minutissima. Nitrogen and phosphorous limited cells (N(L)P(L)) showed maximum lipid productivity (49.1±0.41mg/L/d), 1.47 folds higher than control. Nitrogen depletion resulted in reduced cell size with large sized lipid droplets encompassing most of the intracellular space while discrete lipid bodies were observed under nitrogen sufficiency. Synergistic N/P starvations showed more prominent effect on photosynthetic pigments as to individual deprivations. Phosphorous deficiency along with N starvation exhibited 17.12% decline in carbohydrate while no change in nitrogen sufficient cells were recorded. The optimum N(L)P(L) concentration showed balance between biomass and lipid by maintaining intermediate cell size, pigments, carbohydrate and proteins. FAME profile showed C14-C18 carbon chains in N(L)P(L) cells with biodiesel properties comparable to plant oil methyl esters. Hence, synergistic N/P limitation was effective for enhancing lipid productivity with reduced consumption of nutrients. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/26970694/Synergistic_dynamics_of_nitrogen_and_phosphorous_influences_lipid_productivity_in_Chlorella_minutissima_for_biodiesel_production_ DB - PRIME DP - Unbound Medicine ER -