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Dissect style response to pollination using metabolite profiling in self-compatible and self-incompatible tomato species.

Abstract

Tomato style is the pathway for pollen germination and pollen tubes growth from the stigma to the ovules where fertilization occurs. It is essential to supplying the nutrients for pollen tube growth and guidance for the pollen tubes. To our knowledge, style also regulates gametophytic self-incompatibility (SI) in tomato species. This study identified the metabolites and monitored the metabolic changes of self-incompatible and self-compatible tomato with self-pollinated or unpollinated styles by gas chromatography-mass spectrometry (GC-MS). A total of 9 classes of compounds were identified in SI and self-compatibility (SC) self-pollinated and unpollinated styles which included amino acids, sugars, fatty acids/lipids, amines, organic acids, alcohols, nitriles, inorganic acids and other compounds. The contents of d-Mannose-6-phosphate, Cellobiose, Myristic acid, 2,4-Diaminobutyric acid, Inositol and Urea were significantly decreased and the rest did not significantly change in SI styles. But change of metabolites content significantly happened in SC styles. In addition, among the total 9 classes of compounds, the different metabolites accounted for a different proportion in amino acids, sugars, amines, organic acids and alcohols compared SC and SI. The result indicated that the physiological changes of styles existed differences in SC and SI after self pollination.

Authors+Show Affiliations

Joint Tomato Research Institute, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai 200240, China.Joint Tomato Research Institute, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: lxzhao@sjtu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26974868

Citation

Zhao, Panfeng, et al. "Dissect Style Response to Pollination Using Metabolite Profiling in Self-compatible and Self-incompatible Tomato Species." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 1017-1018, 2016, pp. 153-162.
Zhao P, Pan Q, Yu W, et al. Dissect style response to pollination using metabolite profiling in self-compatible and self-incompatible tomato species. J Chromatogr B Analyt Technol Biomed Life Sci. 2016;1017-1018:153-162.
Zhao, P., Pan, Q., Yu, W., & Zhao, L. (2016). Dissect style response to pollination using metabolite profiling in self-compatible and self-incompatible tomato species. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1017-1018, pp. 153-162. doi:10.1016/j.jchromb.2016.01.056.
Zhao P, et al. Dissect Style Response to Pollination Using Metabolite Profiling in Self-compatible and Self-incompatible Tomato Species. J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Apr 1;1017-1018:153-162. PubMed PMID: 26974868.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dissect style response to pollination using metabolite profiling in self-compatible and self-incompatible tomato species. AU - Zhao,Panfeng, AU - Pan,Qifang, AU - Yu,Wengjuan, AU - Zhao,Lingxia, Y1 - 2016/02/23/ PY - 2015/10/03/received PY - 2016/01/18/revised PY - 2016/01/30/accepted PY - 2016/3/15/entrez PY - 2016/3/15/pubmed PY - 2016/12/15/medline KW - GC–MS KW - Metabolomics KW - Self-compatibility KW - Self-incompatibility KW - Style KW - Tomato SP - 153 EP - 162 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 1017-1018 N2 - Tomato style is the pathway for pollen germination and pollen tubes growth from the stigma to the ovules where fertilization occurs. It is essential to supplying the nutrients for pollen tube growth and guidance for the pollen tubes. To our knowledge, style also regulates gametophytic self-incompatibility (SI) in tomato species. This study identified the metabolites and monitored the metabolic changes of self-incompatible and self-compatible tomato with self-pollinated or unpollinated styles by gas chromatography-mass spectrometry (GC-MS). A total of 9 classes of compounds were identified in SI and self-compatibility (SC) self-pollinated and unpollinated styles which included amino acids, sugars, fatty acids/lipids, amines, organic acids, alcohols, nitriles, inorganic acids and other compounds. The contents of d-Mannose-6-phosphate, Cellobiose, Myristic acid, 2,4-Diaminobutyric acid, Inositol and Urea were significantly decreased and the rest did not significantly change in SI styles. But change of metabolites content significantly happened in SC styles. In addition, among the total 9 classes of compounds, the different metabolites accounted for a different proportion in amino acids, sugars, amines, organic acids and alcohols compared SC and SI. The result indicated that the physiological changes of styles existed differences in SC and SI after self pollination. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/26974868/Dissect_style_response_to_pollination_using_metabolite_profiling_in_self_compatible_and_self_incompatible_tomato_species_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(16)30063-0 DB - PRIME DP - Unbound Medicine ER -