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Detection of virulence-associated genes in pathogenic and commensal avian Escherichia coli isolates.
Poult Sci. 2016 Jul 01; 95(7):1646-52.PS

Abstract

Poultry colibacillosis due to Avian Pathogenic Escherichia coli (APEC) is responsible for several extra-intestinal pathological conditions, leading to serious economic damage in poultry production. The most commonly associated pathologies are airsacculitis, colisepticemia, and cellulitis in broiler chickens, and salpingitis and peritonitis in broiler breeders. In this work a total of 66 strains isolated from dead broiler breeders affected with colibacillosis and 61 strains from healthy broilers were studied. Strains from broiler breeders were typified with serogroups O2, O18, and O78, which are mainly associated with disease. The serogroup O78 was the most prevalent (58%). All the strains were checked for the presence of 11 virulence genes: 1) arginine succinyltransferase A (astA); ii) E.coli hemeutilization protein A (chuA); iii) colicin V A/B (cvaA/B); iv) fimbriae mannose-binding type 1 (fimC); v) ferric yersiniabactin uptake A (fyuA); vi) iron-repressible high-molecular-weight proteins 2 (irp2); vii) increased serum survival (iss); viii) iron-uptake systems of E.coli D (iucD); ix) pielonefritis associated to pili C (papC); x) temperature sensitive haemaglutinin (tsh), and xi) vacuolating autotransporter toxin (vat), by Multiplex-PCR. The results showed that all genes are present in both commensal and pathogenic E. coli strains. The iron uptake-related genes and the serum survival gene were more prevalent among APEC. The adhesin genes, except tsh, and the toxin genes, except astA, were also more prevalent among APEC isolates. Except for astA and tsh, APEC strains harbored the majority of the virulence-associated genes studied and fimC was the most prevalent gene, detected in 96.97 and 88.52% of APEC and AFEC strains, respectively. Possession of more than one iron transport system seems to play an important role on APEC survival.

Authors+Show Affiliations

Department of Chemistry, School of Sciences and Technology, University of Évora. 7000-093 Évora, Portugal anaraquelpaixao@sapo.pt.Instituto Nacional de Investigação Agrária e Veterinária (INIAV). Rua General Morais Sarmento, s/n 1500-311 Lisboa, Portugal.Sociedade Agrícola da Quinta da Freiria, Valouro Group. Quinta da Freiria, Roliça, 2540-671 Roliça, Portugal.Instituto Nacional de Investigação Agrária e Veterinária (INIAV). Rua General Morais Sarmento, s/n 1500-311 Lisboa, Portugal.Instituto Nacional de Investigação Agrária e Veterinária (INIAV). Rua General Morais Sarmento, s/n 1500-311 Lisboa, Portugal.Sociedade Agrícola da Quinta da Freiria, Valouro Group. Quinta da Freiria, Roliça, 2540-671 Roliça, Portugal.Instituto Nacional de Investigação Agrária e Veterinária (INIAV). Rua General Morais Sarmento, s/n 1500-311 Lisboa, Portugal.Department of Veterinary Medicine, School of Sciences and Technology, and Veterinary Hospital - University of Évora. Núcleo da Mitra, 7000-093 Évora, Portugal Institute of Mediterranean Agricultural and Environmental Science (ICAAM), University of Évora. Núcleo da Mitra, Ap. 94, 7002-554 Évora, Portugal.Instituto Nacional de Investigação Agrária e Veterinária (INIAV). Rua General Morais Sarmento, s/n 1500-311 Lisboa, Portugal.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26976911

Citation

Paixão, A C., et al. "Detection of Virulence-associated Genes in Pathogenic and Commensal Avian Escherichia Coli Isolates." Poultry Science, vol. 95, no. 7, 2016, pp. 1646-52.
Paixão AC, Ferreira AC, Fontes M, et al. Detection of virulence-associated genes in pathogenic and commensal avian Escherichia coli isolates. Poult Sci. 2016;95(7):1646-52.
Paixão, A. C., Ferreira, A. C., Fontes, M., Themudo, P., Albuquerque, T., Soares, M. C., Fevereiro, M., Martins, L., & Corrêa de Sá, M. I. (2016). Detection of virulence-associated genes in pathogenic and commensal avian Escherichia coli isolates. Poultry Science, 95(7), 1646-52. https://doi.org/10.3382/ps/pew087
Paixão AC, et al. Detection of Virulence-associated Genes in Pathogenic and Commensal Avian Escherichia Coli Isolates. Poult Sci. 2016 Jul 1;95(7):1646-52. PubMed PMID: 26976911.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of virulence-associated genes in pathogenic and commensal avian Escherichia coli isolates. AU - Paixão,A C, AU - Ferreira,A C, AU - Fontes,M, AU - Themudo,P, AU - Albuquerque,T, AU - Soares,M C, AU - Fevereiro,M, AU - Martins,L, AU - Corrêa de Sá,M I, Y1 - 2016/03/14/ PY - 2016/02/02/accepted PY - 2015/10/27/received PY - 2016/3/16/entrez PY - 2016/3/16/pubmed PY - 2017/4/7/medline KW - Avian Faecal Escherichia coli (AFEC) KW - Avian Pathogenic Escherichia coli (APEC) KW - Multiplex-PCR KW - virulence-associated genes (VAG) SP - 1646 EP - 52 JF - Poultry science JO - Poult. Sci. VL - 95 IS - 7 N2 - Poultry colibacillosis due to Avian Pathogenic Escherichia coli (APEC) is responsible for several extra-intestinal pathological conditions, leading to serious economic damage in poultry production. The most commonly associated pathologies are airsacculitis, colisepticemia, and cellulitis in broiler chickens, and salpingitis and peritonitis in broiler breeders. In this work a total of 66 strains isolated from dead broiler breeders affected with colibacillosis and 61 strains from healthy broilers were studied. Strains from broiler breeders were typified with serogroups O2, O18, and O78, which are mainly associated with disease. The serogroup O78 was the most prevalent (58%). All the strains were checked for the presence of 11 virulence genes: 1) arginine succinyltransferase A (astA); ii) E.coli hemeutilization protein A (chuA); iii) colicin V A/B (cvaA/B); iv) fimbriae mannose-binding type 1 (fimC); v) ferric yersiniabactin uptake A (fyuA); vi) iron-repressible high-molecular-weight proteins 2 (irp2); vii) increased serum survival (iss); viii) iron-uptake systems of E.coli D (iucD); ix) pielonefritis associated to pili C (papC); x) temperature sensitive haemaglutinin (tsh), and xi) vacuolating autotransporter toxin (vat), by Multiplex-PCR. The results showed that all genes are present in both commensal and pathogenic E. coli strains. The iron uptake-related genes and the serum survival gene were more prevalent among APEC. The adhesin genes, except tsh, and the toxin genes, except astA, were also more prevalent among APEC isolates. Except for astA and tsh, APEC strains harbored the majority of the virulence-associated genes studied and fimC was the most prevalent gene, detected in 96.97 and 88.52% of APEC and AFEC strains, respectively. Possession of more than one iron transport system seems to play an important role on APEC survival. SN - 1525-3171 UR - https://www.unboundmedicine.com/medline/citation/26976911/Detection_of_virulence_associated_genes_in_pathogenic_and_commensal_avian_Escherichia_coli_isolates_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0032-5791(19)31862-0 DB - PRIME DP - Unbound Medicine ER -