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A Brazilian Marseillevirus Is the Founding Member of a Lineage in Family Marseilleviridae.
Viruses. 2016 Mar 10; 8(3):76.V

Abstract

In 2003, Acanthamoeba polyphaga mimivirus (APMV) was discovered as parasitizing Acanthamoeba. It was revealed to exhibit remarkable features, especially odd genomic characteristics, and founded viral family Mimiviridae. Subsequently, a second family of giant amoebal viruses was described, Marseilleviridae, whose prototype member is Marseillevirus, discovered in 2009. Currently, the genomes of seven different members of this family have been fully sequenced. Previous phylogenetic analysis suggested the existence of three Marseilleviridae lineages: A, B and C. Here, we describe a new member of this family, Brazilian Marseillevirus (BrMV), which was isolated from a Brazilian sample and whose genome was fully sequenced and analyzed. Surprisingly, data from phylogenetic analyses and comparative genomics, including mean amino acid identity between BrMV and other Marseilleviridae members and the analyses of the core genome and pan-genome of marseilleviruses, indicated that this virus can be assigned to a new Marseilleviridae lineage. Even if the BrMV genome is one of the smallest among Marseilleviridae members, it harbors the second largest gene content into this family. In addition, the BrMV genome encodes 29 ORFans. Here, we describe the isolation and genome analyses of the BrMV strain, and propose its classification as the prototype virus of a new lineage D within the family Marseilleviridae.

Authors+Show Affiliations

Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil. fabiopiod154@gmail.com.Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil. felipelopesassis@gmail.com.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille Univ., 27 boulevard Jean Moulin, Faculté de Médecine, Marseille 13385, France. aherfi.s@gmail.com. Fondation Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Assistance Publique-Hôpitaux de Marseille, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Centre Hospitalo-Universitaire Timone, Fédération de Bactériologie-Hygiène-Virologie, 264 rue Saint-Pierre, Marseille 13385, France. aherfi.s@gmail.com.Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil. tsarantes@gmail.com.Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil. jonatas.abrahao@gmail.com.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille Univ., 27 boulevard Jean Moulin, Faculté de Médecine, Marseille 13385, France. philippe.colson@univ-amu.fr. Fondation Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Assistance Publique-Hôpitaux de Marseille, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Centre Hospitalo-Universitaire Timone, Fédération de Bactériologie-Hygiène-Virologie, 264 rue Saint-Pierre, Marseille 13385, France. philippe.colson@univ-amu.fr.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille Univ., 27 boulevard Jean Moulin, Faculté de Médecine, Marseille 13385, France. bernard.la-scola@univ-amu.fr. Fondation Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Assistance Publique-Hôpitaux de Marseille, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Centre Hospitalo-Universitaire Timone, Fédération de Bactériologie-Hygiène-Virologie, 264 rue Saint-Pierre, Marseille 13385, France. bernard.la-scola@univ-amu.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26978387

Citation

Dornas, Fábio P., et al. "A Brazilian Marseillevirus Is the Founding Member of a Lineage in Family Marseilleviridae." Viruses, vol. 8, no. 3, 2016, p. 76.
Dornas FP, Assis FL, Aherfi S, et al. A Brazilian Marseillevirus Is the Founding Member of a Lineage in Family Marseilleviridae. Viruses. 2016;8(3):76.
Dornas, F. P., Assis, F. L., Aherfi, S., Arantes, T., Abrahão, J. S., Colson, P., & La Scola, B. (2016). A Brazilian Marseillevirus Is the Founding Member of a Lineage in Family Marseilleviridae. Viruses, 8(3), 76. https://doi.org/10.3390/v8030076
Dornas FP, et al. A Brazilian Marseillevirus Is the Founding Member of a Lineage in Family Marseilleviridae. Viruses. 2016 Mar 10;8(3):76. PubMed PMID: 26978387.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Brazilian Marseillevirus Is the Founding Member of a Lineage in Family Marseilleviridae. AU - Dornas,Fábio P, AU - Assis,Felipe L, AU - Aherfi,Sarah, AU - Arantes,Thalita, AU - Abrahão,Jônatas S, AU - Colson,Philippe, AU - La Scola,Bernard, Y1 - 2016/03/10/ PY - 2015/12/17/received PY - 2016/02/22/revised PY - 2016/02/29/accepted PY - 2016/3/16/entrez PY - 2016/3/16/pubmed PY - 2016/11/8/medline KW - Brazilian marseillevirus KW - Marseilleviridae KW - Marseillevirus KW - genomic analyses KW - giant virus KW - lineage D SP - 76 EP - 76 JF - Viruses JO - Viruses VL - 8 IS - 3 N2 - In 2003, Acanthamoeba polyphaga mimivirus (APMV) was discovered as parasitizing Acanthamoeba. It was revealed to exhibit remarkable features, especially odd genomic characteristics, and founded viral family Mimiviridae. Subsequently, a second family of giant amoebal viruses was described, Marseilleviridae, whose prototype member is Marseillevirus, discovered in 2009. Currently, the genomes of seven different members of this family have been fully sequenced. Previous phylogenetic analysis suggested the existence of three Marseilleviridae lineages: A, B and C. Here, we describe a new member of this family, Brazilian Marseillevirus (BrMV), which was isolated from a Brazilian sample and whose genome was fully sequenced and analyzed. Surprisingly, data from phylogenetic analyses and comparative genomics, including mean amino acid identity between BrMV and other Marseilleviridae members and the analyses of the core genome and pan-genome of marseilleviruses, indicated that this virus can be assigned to a new Marseilleviridae lineage. Even if the BrMV genome is one of the smallest among Marseilleviridae members, it harbors the second largest gene content into this family. In addition, the BrMV genome encodes 29 ORFans. Here, we describe the isolation and genome analyses of the BrMV strain, and propose its classification as the prototype virus of a new lineage D within the family Marseilleviridae. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/26978387/A_Brazilian_Marseillevirus_Is_the_Founding_Member_of_a_Lineage_in_Family_Marseilleviridae_ L2 - http://www.mdpi.com/resolver?pii=v8030076 DB - PRIME DP - Unbound Medicine ER -