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Molecular characterization of a novel reassortant H1N2 influenza virus containing genes from the 2009 pandemic human H1N1 virus in swine from eastern China.
Virus Genes 2016; 52(3):405-10VG

Abstract

Pandemic outbreaks of H1N1 swine influenza virus have been reported since 2009. Reassortant H1N2 viruses that contain genes from the pandemic H1N1 virus have been isolated in Italy and the United States. However, there is limited information regarding the molecular characteristics of reassortant H1N2 swine influenza viruses in eastern China. Active influenza surveillance programs in Zhejiang Province identified a novel H1N2 influenza virus isolated from pigs displaying clinical signs of influenza virus infection. Whole-genome sequencing was performed and this strain was compared with other influenza viruses available in GenBank. Phylogenetic analysis suggested that the novel strain contained genes from the 2009 pandemic human H1N1 and swine H3N2 viruses. BALB/c mice were infected with the isolated virus to assess its virulence in mice. While the novel H1N2 isolate replicated well in mice, it was found to be less virulent. These results provide additional evidence that swine serve as intermediate hosts or 'mixing vessels' for novel influenza viruses. They also emphasize the importance of surveillance in the swine population for use as an early warning system for influenza outbreaks in swine and human populations.

Authors+Show Affiliations

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.Animal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Science, Hangzhou, 310021, Zhejiang, China.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. flwnp2013@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26980674

Citation

Peng, Xiuming, et al. "Molecular Characterization of a Novel Reassortant H1N2 Influenza Virus Containing Genes From the 2009 Pandemic Human H1N1 Virus in Swine From Eastern China." Virus Genes, vol. 52, no. 3, 2016, pp. 405-10.
Peng X, Wu H, Xu L, et al. Molecular characterization of a novel reassortant H1N2 influenza virus containing genes from the 2009 pandemic human H1N1 virus in swine from eastern China. Virus Genes. 2016;52(3):405-10.
Peng, X., Wu, H., Xu, L., Peng, X., Cheng, L., Jin, C., ... Wu, N. (2016). Molecular characterization of a novel reassortant H1N2 influenza virus containing genes from the 2009 pandemic human H1N1 virus in swine from eastern China. Virus Genes, 52(3), pp. 405-10. doi:10.1007/s11262-016-1303-4.
Peng X, et al. Molecular Characterization of a Novel Reassortant H1N2 Influenza Virus Containing Genes From the 2009 Pandemic Human H1N1 Virus in Swine From Eastern China. Virus Genes. 2016;52(3):405-10. PubMed PMID: 26980674.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of a novel reassortant H1N2 influenza virus containing genes from the 2009 pandemic human H1N1 virus in swine from eastern China. AU - Peng,Xiuming, AU - Wu,Haibo, AU - Xu,Lihua, AU - Peng,Xiaorong, AU - Cheng,Linfang, AU - Jin,Changzhong, AU - Xie,Tiansheng, AU - Lu,Xiangyun, AU - Wu,Nanping, Y1 - 2016/03/15/ PY - 2015/10/22/received PY - 2016/02/13/accepted PY - 2016/3/17/entrez PY - 2016/3/17/pubmed PY - 2017/4/14/medline KW - Eastern China KW - H1N1 KW - H1N2 KW - Swine influenza virus KW - Virus reassortment SP - 405 EP - 10 JF - Virus genes JO - Virus Genes VL - 52 IS - 3 N2 - Pandemic outbreaks of H1N1 swine influenza virus have been reported since 2009. Reassortant H1N2 viruses that contain genes from the pandemic H1N1 virus have been isolated in Italy and the United States. However, there is limited information regarding the molecular characteristics of reassortant H1N2 swine influenza viruses in eastern China. Active influenza surveillance programs in Zhejiang Province identified a novel H1N2 influenza virus isolated from pigs displaying clinical signs of influenza virus infection. Whole-genome sequencing was performed and this strain was compared with other influenza viruses available in GenBank. Phylogenetic analysis suggested that the novel strain contained genes from the 2009 pandemic human H1N1 and swine H3N2 viruses. BALB/c mice were infected with the isolated virus to assess its virulence in mice. While the novel H1N2 isolate replicated well in mice, it was found to be less virulent. These results provide additional evidence that swine serve as intermediate hosts or 'mixing vessels' for novel influenza viruses. They also emphasize the importance of surveillance in the swine population for use as an early warning system for influenza outbreaks in swine and human populations. SN - 1572-994X UR - https://www.unboundmedicine.com/medline/citation/26980674/Molecular_characterization_of_a_novel_reassortant_H1N2_influenza_virus_containing_genes_from_the_2009_pandemic_human_H1N1_virus_in_swine_from_eastern_China_ L2 - https://doi.org/10.1007/s11262-016-1303-4 DB - PRIME DP - Unbound Medicine ER -