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Effects of N-acetylcysteine and pentoxifylline on remote lung injury in a rat model of hind-limb ischemia/reperfusion injury.
J Bras Pneumol. 2016 Jan-Feb; 42(1):9-14.JB

Abstract

OBJECTIVE

To investigate the effects of N-acetylcysteine (NAC) and pentoxifylline in a model of remote organ injury after hind-limb ischemia/reperfusion (I/R) in rats, the lungs being the remote organ system.

METHODS

Thirty-five male Wistar rats were assigned to one of five conditions (n = 7/group), as follows: sham operation (control group); hind-limb ischemia, induced by clamping the left femoral artery, for 2 h, followed by 24 h of reperfusion (I/R group); and hind-limb ischemia, as above, followed by intraperitoneal injection (prior to reperfusion) of 150 mg/kg of NAC (I/R+NAC group), 40 mg/kg of pentoxifylline (I/R+PTX group), or both (I/R+NAC+PTX group). At the end of the trial, lung tissues were removed for histological analysis and assessment of oxidative stress.

RESULTS

In comparison with the rats in the other groups, those in the I/R group showed lower superoxide dismutase activity and glutathione levels, together with higher malondialdehyde levels and lung injury scores (p < 0.05 for all). Interstitial inflammatory cell infiltration of the lungs was also markedly greater in the I/R group than in the other groups. In addition, I/R group rats showed various signs of interstitial edema and hemorrhage. In the I/R+NAC, I/R+PTX, and I/R+NAC+PTX groups, superoxide dismutase activity, glutathione levels, malondialdehyde levels, and lung injury scores were preserved (p < 0.05 for all). The differences between the administration of NAC or pentoxifylline alone and the administration of the two together were not significant for any of those parameters (p > 0.05 for all).

CONCLUSIONS

Our results suggest that NAC and pentoxifylline both protect lung tissue from the effects of skeletal muscle I/R. However, their combined use does not appear to increase the level of that protection.

Authors+Show Affiliations

Islamic Azad University, Tehran, Iran.Islamic Azad University, Tehran, Iran.Novin Pet Clinic, Isfahan, Iran.Islamic Azad University, Tehran, Iran.Islamic Azad University, Tehran, Iran.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng por

PubMed ID

26982035

Citation

Takhtfooladi, Hamed Ashrafzadeh, et al. "Effects of N-acetylcysteine and Pentoxifylline On Remote Lung Injury in a Rat Model of Hind-limb Ischemia/reperfusion Injury." Jornal Brasileiro De Pneumologia : Publicacao Oficial Da Sociedade Brasileira De Pneumologia E Tisilogia, vol. 42, no. 1, 2016, pp. 9-14.
Takhtfooladi HA, Hesaraki S, Razmara F, et al. Effects of N-acetylcysteine and pentoxifylline on remote lung injury in a rat model of hind-limb ischemia/reperfusion injury. J Bras Pneumol. 2016;42(1):9-14.
Takhtfooladi, H. A., Hesaraki, S., Razmara, F., Takhtfooladi, M. A., & Hajizadeh, H. (2016). Effects of N-acetylcysteine and pentoxifylline on remote lung injury in a rat model of hind-limb ischemia/reperfusion injury. Jornal Brasileiro De Pneumologia : Publicacao Oficial Da Sociedade Brasileira De Pneumologia E Tisilogia, 42(1), 9-14. https://doi.org/10.1590/S1806-37562016000000183
Takhtfooladi HA, et al. Effects of N-acetylcysteine and Pentoxifylline On Remote Lung Injury in a Rat Model of Hind-limb Ischemia/reperfusion Injury. J Bras Pneumol. 2016 Jan-Feb;42(1):9-14. PubMed PMID: 26982035.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of N-acetylcysteine and pentoxifylline on remote lung injury in a rat model of hind-limb ischemia/reperfusion injury. AU - Takhtfooladi,Hamed Ashrafzadeh, AU - Hesaraki,Saeed, AU - Razmara,Foad, AU - Takhtfooladi,Mohammad Ashrafzadeh, AU - Hajizadeh,Hadi, PY - 2015/07/29/received PY - 2015/11/04/accepted PY - 2016/3/17/entrez PY - 2016/3/18/pubmed PY - 2016/11/15/medline SP - 9 EP - 14 JF - Jornal brasileiro de pneumologia : publicacao oficial da Sociedade Brasileira de Pneumologia e Tisilogia JO - J Bras Pneumol VL - 42 IS - 1 N2 - OBJECTIVE: To investigate the effects of N-acetylcysteine (NAC) and pentoxifylline in a model of remote organ injury after hind-limb ischemia/reperfusion (I/R) in rats, the lungs being the remote organ system. METHODS: Thirty-five male Wistar rats were assigned to one of five conditions (n = 7/group), as follows: sham operation (control group); hind-limb ischemia, induced by clamping the left femoral artery, for 2 h, followed by 24 h of reperfusion (I/R group); and hind-limb ischemia, as above, followed by intraperitoneal injection (prior to reperfusion) of 150 mg/kg of NAC (I/R+NAC group), 40 mg/kg of pentoxifylline (I/R+PTX group), or both (I/R+NAC+PTX group). At the end of the trial, lung tissues were removed for histological analysis and assessment of oxidative stress. RESULTS: In comparison with the rats in the other groups, those in the I/R group showed lower superoxide dismutase activity and glutathione levels, together with higher malondialdehyde levels and lung injury scores (p < 0.05 for all). Interstitial inflammatory cell infiltration of the lungs was also markedly greater in the I/R group than in the other groups. In addition, I/R group rats showed various signs of interstitial edema and hemorrhage. In the I/R+NAC, I/R+PTX, and I/R+NAC+PTX groups, superoxide dismutase activity, glutathione levels, malondialdehyde levels, and lung injury scores were preserved (p < 0.05 for all). The differences between the administration of NAC or pentoxifylline alone and the administration of the two together were not significant for any of those parameters (p > 0.05 for all). CONCLUSIONS: Our results suggest that NAC and pentoxifylline both protect lung tissue from the effects of skeletal muscle I/R. However, their combined use does not appear to increase the level of that protection. SN - 1806-3756 UR - https://www.unboundmedicine.com/medline/citation/26982035/Effects_of_N_acetylcysteine_and_pentoxifylline_on_remote_lung_injury_in_a_rat_model_of_hind_limb_ischemia/reperfusion_injury_ L2 - https://www.scielo.br/scielo.php?script=sci_arttext&amp;pid=S1806-37132016000100009&amp;lng=en&amp;nrm=iso&amp;tlng=en DB - PRIME DP - Unbound Medicine ER -