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[Value of serum osteoprotegerin in noninvasive diagnosis of nonalcoholic steatohepatitis].
Zhonghua Gan Zang Bing Za Zhi 2016; 24(2):96-101ZG

Abstract

OBJECTIVE

To investigate the correlation of serum osteoprotegerin (OPG) with the progression of nonalcoholic fatty liver disease (NAFLD) and the noninvasive prediction and diagnosis of nonalcoholic steatohepatitis (NASH).

METHODS

A total of 136 patients with NAFLD were enrolled, and their tissue samples for liver biopsy and serum samples obtained at 1 week after liver biopsy were collected; 83 healthy subjects without the symptoms of fatty liver disease proved by ultrasound examination were enrolled as controls. The physiological indicators including height, body weight, and waist circumference were measured, and body mass index was calculated. The biochemical parameters including alanine aminotransferase (ALT), aspartate aminotransferase (AST), AST/ALT, alkaline phosphatase, gamma-glutamyl transferase, total cholesterol, triglyceride (TG), high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were measured. Double-antibody sandwich enzyme-linked immunosorbent assay was used to determine the serum level of OPG. The rank sum test, chi-square test, t-test, one-way analysis of variance, Spearman correlation analysis, least significant difference test, and receiver operating characteristic (ROC) curve were applied for statistical analysis of various data.

RESULTS

Serum OPG level was correlated with AST and TG (P < 0.05), and was highly correlated with hepatocyte fatty degeneration, ballooning degeneration, intralobular inflammation, portal inflammation, and fibrosis degree (P < 0.01). With the increasing NAFLD activity score (NAS), serum OPG level decreased, and there was a highly negative correlation between them (r = -0.928, P < 0.01). Serum OPG level was significantly lower in NASH patients than non-NASH patients. The area under the ROC curve of serum OPG level was 0.963, and according to the Youden index, its optimal sensitivity and specificity were 96.1% and 97.4%, respectively, at an optimal cut-off value of 242.96 ng/L, which suggested a high diagnostic power.

CONCLUSION

In NASH patients, serum OPG level decreases significantly. Serum OPG level can be used as an independent predictive factor to evaluate NASH and its severity, as well as a noninvasive diagnostic index for NASH.

Authors+Show Affiliations

Department of Pathology and Hepatology, 302 Military Hospital of China, Beijing 100039, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26983475

Citation

Yang, Mei, et al. "[Value of Serum Osteoprotegerin in Noninvasive Diagnosis of Nonalcoholic Steatohepatitis]." Zhonghua Gan Zang Bing Za Zhi = Zhonghua Ganzangbing Zazhi = Chinese Journal of Hepatology, vol. 24, no. 2, 2016, pp. 96-101.
Yang M, Liu Y, Zhou G, et al. [Value of serum osteoprotegerin in noninvasive diagnosis of nonalcoholic steatohepatitis]. Zhonghua Gan Zang Bing Za Zhi. 2016;24(2):96-101.
Yang, M., Liu, Y., Zhou, G., Guo, X., Zou, S., Liu, S., ... Zhao, J. (2016). [Value of serum osteoprotegerin in noninvasive diagnosis of nonalcoholic steatohepatitis]. Zhonghua Gan Zang Bing Za Zhi = Zhonghua Ganzangbing Zazhi = Chinese Journal of Hepatology, 24(2), pp. 96-101. doi:10.3760/cma.j.issn.1007-3418.2016.02.005.
Yang M, et al. [Value of Serum Osteoprotegerin in Noninvasive Diagnosis of Nonalcoholic Steatohepatitis]. Zhonghua Gan Zang Bing Za Zhi. 2016;24(2):96-101. PubMed PMID: 26983475.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Value of serum osteoprotegerin in noninvasive diagnosis of nonalcoholic steatohepatitis]. AU - Yang,Mei, AU - Liu,Yisi, AU - Zhou,Guangde, AU - Guo,Xiaodong, AU - Zou,Saiying, AU - Liu,Shuhong, AU - Jiang,Lina, AU - Liu,Yuan, AU - Zhu,Li, AU - Guo,Chaonan, AU - Zhao,Jingmin, PY - 2016/3/18/entrez PY - 2016/3/18/pubmed PY - 2016/6/17/medline SP - 96 EP - 101 JF - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology JO - Zhonghua Gan Zang Bing Za Zhi VL - 24 IS - 2 N2 - OBJECTIVE: To investigate the correlation of serum osteoprotegerin (OPG) with the progression of nonalcoholic fatty liver disease (NAFLD) and the noninvasive prediction and diagnosis of nonalcoholic steatohepatitis (NASH). METHODS: A total of 136 patients with NAFLD were enrolled, and their tissue samples for liver biopsy and serum samples obtained at 1 week after liver biopsy were collected; 83 healthy subjects without the symptoms of fatty liver disease proved by ultrasound examination were enrolled as controls. The physiological indicators including height, body weight, and waist circumference were measured, and body mass index was calculated. The biochemical parameters including alanine aminotransferase (ALT), aspartate aminotransferase (AST), AST/ALT, alkaline phosphatase, gamma-glutamyl transferase, total cholesterol, triglyceride (TG), high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were measured. Double-antibody sandwich enzyme-linked immunosorbent assay was used to determine the serum level of OPG. The rank sum test, chi-square test, t-test, one-way analysis of variance, Spearman correlation analysis, least significant difference test, and receiver operating characteristic (ROC) curve were applied for statistical analysis of various data. RESULTS: Serum OPG level was correlated with AST and TG (P < 0.05), and was highly correlated with hepatocyte fatty degeneration, ballooning degeneration, intralobular inflammation, portal inflammation, and fibrosis degree (P < 0.01). With the increasing NAFLD activity score (NAS), serum OPG level decreased, and there was a highly negative correlation between them (r = -0.928, P < 0.01). Serum OPG level was significantly lower in NASH patients than non-NASH patients. The area under the ROC curve of serum OPG level was 0.963, and according to the Youden index, its optimal sensitivity and specificity were 96.1% and 97.4%, respectively, at an optimal cut-off value of 242.96 ng/L, which suggested a high diagnostic power. CONCLUSION: In NASH patients, serum OPG level decreases significantly. Serum OPG level can be used as an independent predictive factor to evaluate NASH and its severity, as well as a noninvasive diagnostic index for NASH. SN - 1007-3418 UR - https://www.unboundmedicine.com/medline/citation/26983475/[Value_of_serum_osteoprotegerin_in_noninvasive_diagnosis_of_nonalcoholic_steatohepatitis]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=1007-3418&amp;year=2016&amp;vol=24&amp;issue=2&amp;fpage=96 DB - PRIME DP - Unbound Medicine ER -