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Reciprocal regulation of Yersinia pestis biofilm formation and virulence by RovM and RovA.
Open Biol. 2016 Mar; 6(3)OB

Abstract

RovA is known to enhance Yersinia pestis virulence by directly upregulating the psa loci. This work presents a complex gene regulatory paradigm involving the reciprocal regulatory action of RovM and RovA on the expression of biofilm and virulence genes as well as on their own genes. RovM and RovA enhance and inhibit Y. pestis biofilm production, respectively, whereas RovM represses virulence in mice. RovM directly stimulates the transcription of hmsT, hmsCDE and rovM, while indirectly enhancing hmsHFRS transcription. It also indirectly represses hmsP transcription. By contrast, RovA directly represses hmsT transcription and indirectly inhibits waaAE-coaD transcription, while RovM inhibits psaABC and psaEF transcription by directly repressing rovA transcription. rovM expression is significantly upregulated at 26°C (the temperature of the flea gut) relative to 37°C (the warm-blooded host temperature). We speculate that upregulation of rovM together with downregulation of rovA in the flea gut would promote Y. pestis biofilm formation while inhibiting virulence gene expression, leading to a more transmissible infection of this pathogen in fleas. Once the bacterium shifts to a lifestyle in the warm-blooded hosts, inhibited RovM production accompanied by recovered RovA synthesis would encourage virulence factor production and inhibit biofilm gene expression.

Authors+Show Affiliations

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, People's Republic of China.State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, People's Republic of China.State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, People's Republic of China.State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, People's Republic of China School of Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu, People's Republic of China.State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, People's Republic of China.State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, People's Republic of China dongshengzhou1977@gmail.com.State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, People's Republic of China ruifuyang@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26984293

Citation

Liu, Lei, et al. "Reciprocal Regulation of Yersinia Pestis Biofilm Formation and Virulence By RovM and RovA." Open Biology, vol. 6, no. 3, 2016.
Liu L, Fang H, Yang H, et al. Reciprocal regulation of Yersinia pestis biofilm formation and virulence by RovM and RovA. Open Biol. 2016;6(3).
Liu, L., Fang, H., Yang, H., Zhang, Y., Han, Y., Zhou, D., & Yang, R. (2016). Reciprocal regulation of Yersinia pestis biofilm formation and virulence by RovM and RovA. Open Biology, 6(3). https://doi.org/10.1098/rsob.150198
Liu L, et al. Reciprocal Regulation of Yersinia Pestis Biofilm Formation and Virulence By RovM and RovA. Open Biol. 2016;6(3) PubMed PMID: 26984293.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reciprocal regulation of Yersinia pestis biofilm formation and virulence by RovM and RovA. AU - Liu,Lei, AU - Fang,Haihong, AU - Yang,Huiying, AU - Zhang,Yiquan, AU - Han,Yanping, AU - Zhou,Dongsheng, AU - Yang,Ruifu, PY - 2016/3/18/entrez PY - 2016/3/18/pubmed PY - 2016/12/15/medline KW - RovA KW - RovM KW - Yersinia pestis KW - biofilm KW - transcriptional regulation KW - virulence JF - Open biology JO - Open Biol VL - 6 IS - 3 N2 - RovA is known to enhance Yersinia pestis virulence by directly upregulating the psa loci. This work presents a complex gene regulatory paradigm involving the reciprocal regulatory action of RovM and RovA on the expression of biofilm and virulence genes as well as on their own genes. RovM and RovA enhance and inhibit Y. pestis biofilm production, respectively, whereas RovM represses virulence in mice. RovM directly stimulates the transcription of hmsT, hmsCDE and rovM, while indirectly enhancing hmsHFRS transcription. It also indirectly represses hmsP transcription. By contrast, RovA directly represses hmsT transcription and indirectly inhibits waaAE-coaD transcription, while RovM inhibits psaABC and psaEF transcription by directly repressing rovA transcription. rovM expression is significantly upregulated at 26°C (the temperature of the flea gut) relative to 37°C (the warm-blooded host temperature). We speculate that upregulation of rovM together with downregulation of rovA in the flea gut would promote Y. pestis biofilm formation while inhibiting virulence gene expression, leading to a more transmissible infection of this pathogen in fleas. Once the bacterium shifts to a lifestyle in the warm-blooded hosts, inhibited RovM production accompanied by recovered RovA synthesis would encourage virulence factor production and inhibit biofilm gene expression. SN - 2046-2441 UR - https://www.unboundmedicine.com/medline/citation/26984293/Reciprocal_regulation_of_Yersinia_pestis_biofilm_formation_and_virulence_by_RovM_and_RovA_ L2 - https://royalsocietypublishing.org/doi/10.1098/rsob.150198?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -