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Diversity and dynamics of algal Megaviridae members during a harmful brown tide caused by the pelagophyte, Aureococcus anophagefferens.
FEMS Microbiol Ecol. 2016 May; 92(5):fiw058.FM

Abstract

Many giant dsDNA algal viruses share a common ancestor with Mimivirus--one of the largest viruses, in terms of genetic content. Together, these viruses form the proposed 'Megaviridae' clade of nucleocytoplasmic large DNA viruses. To gauge Megaviridae diversity, we designed degenerate primers targeting the major capsid protein genes of algae-infecting viruses within this group and probed the clade's diversity during the course of a brown tide bloom caused by the harmful pelagophyte,Aureococcus anophagefferens We amplified target sequences in water samples from two distinct locations (Weesuck Creek and Quantuck Bay, NY) covering 12 weeks concurrent with the proliferation and demise of a bloom. In total, 475 amplicons clustered into 145 operational taxonomic units (OTUs) at 97% identity. One OTU contained 19 sequences with ≥97% identity to AaV, a member of the Megaviridae clade that infects A. anophagefferens, suggesting AaV was present during the bloom. Unifrac analysis showed clear temporal patterns in algal Megaviridae dynamics, with a shift in the virus community structure that corresponded to the Aureococcus bloom decline in both locations. Our data provide insights regarding the environmental relevance of algal Megaviridae members and raise important questions regarding their phylodynamics across different environmental gradients.

Authors+Show Affiliations

Department of Microbiology, The University of Tennessee, TN 37996, USA.Department of Microbiology, The University of Tennessee, TN 37996, USA Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA.Department of Microbiology, The University of Tennessee, TN 37996, USA.School of Marine and Atmospheric Sciences, Stony Brook, NY 11794, USA.School of Marine and Atmospheric Sciences, Stony Brook, NY 11794, USA.Department of Microbiology, The University of Tennessee, TN 37996, USA wilhelm@utk.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

26985013

Citation

Moniruzzaman, Mohammad, et al. "Diversity and Dynamics of Algal Megaviridae Members During a Harmful Brown Tide Caused By the Pelagophyte, Aureococcus Anophagefferens." FEMS Microbiology Ecology, vol. 92, no. 5, 2016, pp. fiw058.
Moniruzzaman M, Gann ER, LeCleir GR, et al. Diversity and dynamics of algal Megaviridae members during a harmful brown tide caused by the pelagophyte, Aureococcus anophagefferens. FEMS Microbiol Ecol. 2016;92(5):fiw058.
Moniruzzaman, M., Gann, E. R., LeCleir, G. R., Kang, Y., Gobler, C. J., & Wilhelm, S. W. (2016). Diversity and dynamics of algal Megaviridae members during a harmful brown tide caused by the pelagophyte, Aureococcus anophagefferens. FEMS Microbiology Ecology, 92(5), fiw058. https://doi.org/10.1093/femsec/fiw058
Moniruzzaman M, et al. Diversity and Dynamics of Algal Megaviridae Members During a Harmful Brown Tide Caused By the Pelagophyte, Aureococcus Anophagefferens. FEMS Microbiol Ecol. 2016;92(5):fiw058. PubMed PMID: 26985013.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity and dynamics of algal Megaviridae members during a harmful brown tide caused by the pelagophyte, Aureococcus anophagefferens. AU - Moniruzzaman,Mohammad, AU - Gann,Eric R, AU - LeCleir,Gary R, AU - Kang,Yoonja, AU - Gobler,Christopher J, AU - Wilhelm,Steven W, Y1 - 2016/03/15/ PY - 2016/03/11/accepted PY - 2016/3/18/entrez PY - 2016/3/18/pubmed PY - 2016/11/3/medline KW - Megaviridae KW - brown tides KW - giant viruses KW - viral diversity SP - fiw058 EP - fiw058 JF - FEMS microbiology ecology JO - FEMS Microbiol Ecol VL - 92 IS - 5 N2 - Many giant dsDNA algal viruses share a common ancestor with Mimivirus--one of the largest viruses, in terms of genetic content. Together, these viruses form the proposed 'Megaviridae' clade of nucleocytoplasmic large DNA viruses. To gauge Megaviridae diversity, we designed degenerate primers targeting the major capsid protein genes of algae-infecting viruses within this group and probed the clade's diversity during the course of a brown tide bloom caused by the harmful pelagophyte,Aureococcus anophagefferens We amplified target sequences in water samples from two distinct locations (Weesuck Creek and Quantuck Bay, NY) covering 12 weeks concurrent with the proliferation and demise of a bloom. In total, 475 amplicons clustered into 145 operational taxonomic units (OTUs) at 97% identity. One OTU contained 19 sequences with ≥97% identity to AaV, a member of the Megaviridae clade that infects A. anophagefferens, suggesting AaV was present during the bloom. Unifrac analysis showed clear temporal patterns in algal Megaviridae dynamics, with a shift in the virus community structure that corresponded to the Aureococcus bloom decline in both locations. Our data provide insights regarding the environmental relevance of algal Megaviridae members and raise important questions regarding their phylodynamics across different environmental gradients. SN - 1574-6941 UR - https://www.unboundmedicine.com/medline/citation/26985013/Diversity_and_dynamics_of_algal_Megaviridae_members_during_a_harmful_brown_tide_caused_by_the_pelagophyte_Aureococcus_anophagefferens_ L2 - https://academic.oup.com/femsec/article-lookup/doi/10.1093/femsec/fiw058 DB - PRIME DP - Unbound Medicine ER -