Tags

Type your tag names separated by a space and hit enter

Nutritional regulation of long-chain PUFA biosynthetic genes in rainbow trout (Oncorhynchus mykiss).
Br J Nutr 2016; 115(10):1721-9BJ

Abstract

Most studies on dietary vegetable oil in rainbow trout (Oncorhynchus mykiss) have been conducted on a background of dietary EPA (20 : 5n-3) and DHA (22 : 6n-3) contained in the fishmeal used as a protein source in aquaculture feed. If dietary EPA and DHA repress their endogenous synthesis from α-linolenic acid (ALA, 18 : 3n-3), then the potential of ALA-containing vegetable oils to maintain tissue EPA and DHA has been underestimated. We examined the effect of individual dietary n-3 PUFA on the expression of the biosynthetic genes required for metabolism of ALA to DHA in rainbow trout. A total of 720 juvenile rainbow trout were allocated to twenty-four experimental tanks and assigned one of eight diets. The effect of dietary ALA, EPA or DHA, in isolation or in combination, on hepatic expression of fatty acyl desaturase (FADS)2a(Δ6), FADS2b(Δ5), elongation of very long-chain fatty acid (ELOVL)5 and ELOVL2 was examined after 3 weeks of dietary intervention. The effect of these diets on liver and muscle phospholipid PUFA composition was also examined. The expression levels of FADS2a(Δ6), ELOVL5 and ELOVL2 were highest when diets were high in ALA, with no added EPA or DHA. Under these conditions ALA was readily converted to tissue DHA. Dietary DHA had the largest and most consistent effect in down-regulating the gene expression of all four genes. The ELOVL5 expression was the least responsive of the four genes to dietary n-3 PUFA changes. These findings should be considered when optimising aquaculture feeds containing vegetable oils and/or fish oil or fishmeal to achieve maximum DHA synthesis.

Authors+Show Affiliations

1Rheumatology Unit,Royal Adelaide Hospital,Adelaide,SA 5000,Australia.2School of Life and Environmental Sciences,Deakin University,Warrnambool,VIC 3280,Australia.3School of Natural Sciences,Institute of Aquaculture,University of Stirling,Stirling FK9 4LA,Scotland,UK.1Rheumatology Unit,Royal Adelaide Hospital,Adelaide,SA 5000,Australia.2School of Life and Environmental Sciences,Deakin University,Warrnambool,VIC 3280,Australia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26987422

Citation

Gregory, Melissa K., et al. "Nutritional Regulation of Long-chain PUFA Biosynthetic Genes in Rainbow Trout (Oncorhynchus Mykiss)." The British Journal of Nutrition, vol. 115, no. 10, 2016, pp. 1721-9.
Gregory MK, Collins RO, Tocher DR, et al. Nutritional regulation of long-chain PUFA biosynthetic genes in rainbow trout (Oncorhynchus mykiss). Br J Nutr. 2016;115(10):1721-9.
Gregory, M. K., Collins, R. O., Tocher, D. R., James, M. J., & Turchini, G. M. (2016). Nutritional regulation of long-chain PUFA biosynthetic genes in rainbow trout (Oncorhynchus mykiss). The British Journal of Nutrition, 115(10), pp. 1721-9. doi:10.1017/S0007114516000830.
Gregory MK, et al. Nutritional Regulation of Long-chain PUFA Biosynthetic Genes in Rainbow Trout (Oncorhynchus Mykiss). Br J Nutr. 2016 May 28;115(10):1721-9. PubMed PMID: 26987422.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nutritional regulation of long-chain PUFA biosynthetic genes in rainbow trout (Oncorhynchus mykiss). AU - Gregory,Melissa K, AU - Collins,Robert O, AU - Tocher,Douglas R, AU - James,Michael J, AU - Turchini,Giovanni M, Y1 - 2016/03/18/ PY - 2016/3/19/entrez PY - 2016/3/19/pubmed PY - 2016/9/1/medline KW - ALA α-linolenic acid KW - DHA KW - DPA docosapentaenoic acid KW - Desaturase KW - ELOVL elongation of very long-chain fatty acid KW - Elongase KW - FADS fatty acyl desaturase KW - LC-PUFA long-chain PUFA KW - PUFA KW - n-3 Fatty acids SP - 1721 EP - 9 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 115 IS - 10 N2 - Most studies on dietary vegetable oil in rainbow trout (Oncorhynchus mykiss) have been conducted on a background of dietary EPA (20 : 5n-3) and DHA (22 : 6n-3) contained in the fishmeal used as a protein source in aquaculture feed. If dietary EPA and DHA repress their endogenous synthesis from α-linolenic acid (ALA, 18 : 3n-3), then the potential of ALA-containing vegetable oils to maintain tissue EPA and DHA has been underestimated. We examined the effect of individual dietary n-3 PUFA on the expression of the biosynthetic genes required for metabolism of ALA to DHA in rainbow trout. A total of 720 juvenile rainbow trout were allocated to twenty-four experimental tanks and assigned one of eight diets. The effect of dietary ALA, EPA or DHA, in isolation or in combination, on hepatic expression of fatty acyl desaturase (FADS)2a(Δ6), FADS2b(Δ5), elongation of very long-chain fatty acid (ELOVL)5 and ELOVL2 was examined after 3 weeks of dietary intervention. The effect of these diets on liver and muscle phospholipid PUFA composition was also examined. The expression levels of FADS2a(Δ6), ELOVL5 and ELOVL2 were highest when diets were high in ALA, with no added EPA or DHA. Under these conditions ALA was readily converted to tissue DHA. Dietary DHA had the largest and most consistent effect in down-regulating the gene expression of all four genes. The ELOVL5 expression was the least responsive of the four genes to dietary n-3 PUFA changes. These findings should be considered when optimising aquaculture feeds containing vegetable oils and/or fish oil or fishmeal to achieve maximum DHA synthesis. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/26987422/Nutritional_regulation_of_long_chain_PUFA_biosynthetic_genes_in_rainbow_trout__Oncorhynchus_mykiss__ L2 - https://www.cambridge.org/core/product/identifier/S0007114516000830/type/journal_article DB - PRIME DP - Unbound Medicine ER -