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Photocatalysis-Induced Renewable Field-Effect Transistor for Protein Detection.
Anal Chem. 2016 Apr 05; 88(7):4048-54.AC

Abstract

The field-effect transistor (FET) biosensor has attracted extensive attentions, due to its unique features in detecting various biomolecules with high sensitivity and selectivity. However, currently used FET biosensors obtaining from expensive and elaborate micro/nanofabrication are always disposable because the analyte cannot be efficiently removed after detection. In this work, we established a photocatalysis-induced renewable graphene-FET (G-FET) biosensor for protein detection, by layer-to-layer assembling reduced graphene oxide (RGO) and RGO-encapsulated TiO2 composites to form a sandwiching RGO@TiO2 structure on a prefabricated FET sensor surface. After immobilization of anti-D-Dimer on the graphene surface, sensitive detection of D-Dimer was achieved with the detection limits of 10 pg/mL in PBS and 100 pg/mL in serum, respectively. Notably, renewal of the FET biosensor for recycling measurements was significantly realized by photocatalytically cleaning the substances on the graphene surface. This work demonstrates for the first time the development and application of photocatalytically renewable G-FET biosensor, paving a new way for G-FET sensor toward a plethora of diverse applications.

Authors+Show Affiliations

School of Laboratory Medicine, Hubei University of Chinese Medicine , 1 Huangjia Lake West Road, Wuhan 430065, P.R. China.Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University ,Wuhan 430072, P.R. China.School of Laboratory Medicine, Hubei University of Chinese Medicine , 1 Huangjia Lake West Road, Wuhan 430065, P.R. China.Key Laboratory for the Physics and Chemistry of Nanodevices, Department of Electronics, Peking University , No. 5 Yiheyuan Road, Haidian District, Beijing 100871, P.R. China.Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University ,Wuhan 430072, P.R. China.School of Laboratory Medicine, Hubei University of Chinese Medicine , 1 Huangjia Lake West Road, Wuhan 430065, P.R. China.Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University ,Wuhan 430072, P.R. China.Key Laboratory for the Physics and Chemistry of Nanodevices, Department of Electronics, Peking University , No. 5 Yiheyuan Road, Haidian District, Beijing 100871, P.R. China.School of Laboratory Medicine, Hubei University of Chinese Medicine , 1 Huangjia Lake West Road, Wuhan 430065, P.R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26990067

Citation

Zhang, Changliang, et al. "Photocatalysis-Induced Renewable Field-Effect Transistor for Protein Detection." Analytical Chemistry, vol. 88, no. 7, 2016, pp. 4048-54.
Zhang C, Xu JQ, Li YT, et al. Photocatalysis-Induced Renewable Field-Effect Transistor for Protein Detection. Anal Chem. 2016;88(7):4048-54.
Zhang, C., Xu, J. Q., Li, Y. T., Huang, L., Pang, D. W., Ning, Y., Huang, W. H., Zhang, Z., & Zhang, G. J. (2016). Photocatalysis-Induced Renewable Field-Effect Transistor for Protein Detection. Analytical Chemistry, 88(7), 4048-54. https://doi.org/10.1021/acs.analchem.6b00374
Zhang C, et al. Photocatalysis-Induced Renewable Field-Effect Transistor for Protein Detection. Anal Chem. 2016 Apr 5;88(7):4048-54. PubMed PMID: 26990067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Photocatalysis-Induced Renewable Field-Effect Transistor for Protein Detection. AU - Zhang,Changliang, AU - Xu,Jia-Quan, AU - Li,Yu-Tao, AU - Huang,Le, AU - Pang,Dai-Wen, AU - Ning,Yong, AU - Huang,Wei-Hua, AU - Zhang,Zhiyong, AU - Zhang,Guo-Jun, Y1 - 2016/03/18/ PY - 2016/3/19/entrez PY - 2016/3/19/pubmed PY - 2016/12/16/medline SP - 4048 EP - 54 JF - Analytical chemistry JO - Anal. Chem. VL - 88 IS - 7 N2 - The field-effect transistor (FET) biosensor has attracted extensive attentions, due to its unique features in detecting various biomolecules with high sensitivity and selectivity. However, currently used FET biosensors obtaining from expensive and elaborate micro/nanofabrication are always disposable because the analyte cannot be efficiently removed after detection. In this work, we established a photocatalysis-induced renewable graphene-FET (G-FET) biosensor for protein detection, by layer-to-layer assembling reduced graphene oxide (RGO) and RGO-encapsulated TiO2 composites to form a sandwiching RGO@TiO2 structure on a prefabricated FET sensor surface. After immobilization of anti-D-Dimer on the graphene surface, sensitive detection of D-Dimer was achieved with the detection limits of 10 pg/mL in PBS and 100 pg/mL in serum, respectively. Notably, renewal of the FET biosensor for recycling measurements was significantly realized by photocatalytically cleaning the substances on the graphene surface. This work demonstrates for the first time the development and application of photocatalytically renewable G-FET biosensor, paving a new way for G-FET sensor toward a plethora of diverse applications. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/26990067/Photocatalysis_Induced_Renewable_Field_Effect_Transistor_for_Protein_Detection_ L2 - https://dx.doi.org/10.1021/acs.analchem.6b00374 DB - PRIME DP - Unbound Medicine ER -