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Identification of multiple lipid genes with modifications in expression and sequence associated with the evolution of hydroxy fatty acid accumulation in Physaria fendleri.
Plant J. 2016 05; 86(4):322-48.PJ

Abstract

Two Brassicaceae species, Physaria fendleri and Camelina sativa, are genetically very closely related to each other and to Arabidopsis thaliana. Physaria fendleri seeds contain over 50% hydroxy fatty acids (HFAs), while Camelina sativa and Arabidopsis do not accumulate HFAs. To better understand how plants evolved new biochemical pathways with the capacity to accumulate high levels of unusual fatty acids, transcript expression and protein sequences of developing seeds of Physaria fendleri, wild-type Camelina sativa, and Camelina sativa expressing a castor bean (Ricinus communis) hydroxylase were analyzed. A number of potential evolutionary adaptations within lipid metabolism that probably enhance HFA production and accumulation in Physaria fendleri, and, in their absence, limit accumulation in transgenic tissues were revealed. These adaptations occurred in at least 20 genes within several lipid pathways from the onset of fatty acid synthesis and its regulation to the assembly of triacylglycerols. Lipid genes of Physaria fendleri appear to have co-evolved through modulation of transcriptional abundances and alterations within protein sequences. Only a handful of genes showed evidence for sequence adaptation through gene duplication. Collectively, these evolutionary changes probably occurred to minimize deleterious effects of high HFA amounts and/or to enhance accumulation for physiological advantage. These results shed light on the evolution of pathways for novel fatty acid production in seeds, help explain some of the current limitations to accumulation of HFAs in transgenic plants, and may provide improved strategies for future engineering of their production.

Authors+Show Affiliations

Department of Plant Biology, Michigan State University, East Lansing, Michigan, USA.Department of Plant Biology, Michigan State University, East Lansing, Michigan, USA. Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, Michigan, USA.Department of Molecular Genetics, Ohio State University, Columbus, Ohio, USA.Department of Plant Biology, Michigan State University, East Lansing, Michigan, USA.Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, Michigan, USA.Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, Michigan, USA.Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, Montana, USA.Department of Molecular Genetics, Ohio State University, Columbus, Ohio, USA.Department of Plant Biology, Michigan State University, East Lansing, Michigan, USA. Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, Michigan, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

26991237

Citation

Horn, Patrick J., et al. "Identification of Multiple Lipid Genes With Modifications in Expression and Sequence Associated With the Evolution of Hydroxy Fatty Acid Accumulation in Physaria Fendleri." The Plant Journal : for Cell and Molecular Biology, vol. 86, no. 4, 2016, pp. 322-48.
Horn PJ, Liu J, Cocuron JC, et al. Identification of multiple lipid genes with modifications in expression and sequence associated with the evolution of hydroxy fatty acid accumulation in Physaria fendleri. Plant J. 2016;86(4):322-48.
Horn, P. J., Liu, J., Cocuron, J. C., McGlew, K., Thrower, N. A., Larson, M., Lu, C., Alonso, A. P., & Ohlrogge, J. (2016). Identification of multiple lipid genes with modifications in expression and sequence associated with the evolution of hydroxy fatty acid accumulation in Physaria fendleri. The Plant Journal : for Cell and Molecular Biology, 86(4), 322-48. https://doi.org/10.1111/tpj.13163
Horn PJ, et al. Identification of Multiple Lipid Genes With Modifications in Expression and Sequence Associated With the Evolution of Hydroxy Fatty Acid Accumulation in Physaria Fendleri. Plant J. 2016;86(4):322-48. PubMed PMID: 26991237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of multiple lipid genes with modifications in expression and sequence associated with the evolution of hydroxy fatty acid accumulation in Physaria fendleri. AU - Horn,Patrick J, AU - Liu,Jinjie, AU - Cocuron,Jean-Christophe, AU - McGlew,Kathleen, AU - Thrower,Nicholas A, AU - Larson,Matt, AU - Lu,Chaofu, AU - Alonso,Ana P, AU - Ohlrogge,John, PY - 2015/07/14/received PY - 2016/03/02/revised PY - 2016/03/07/accepted PY - 2016/3/19/entrez PY - 2016/3/19/pubmed PY - 2017/3/28/medline KW - Camelina sativa KW - Physaria fendleri KW - RNA-Seq KW - evolution KW - hydroxy fatty acids KW - metabolic engineering KW - oilseeds KW - transcriptomics SP - 322 EP - 48 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 86 IS - 4 N2 - Two Brassicaceae species, Physaria fendleri and Camelina sativa, are genetically very closely related to each other and to Arabidopsis thaliana. Physaria fendleri seeds contain over 50% hydroxy fatty acids (HFAs), while Camelina sativa and Arabidopsis do not accumulate HFAs. To better understand how plants evolved new biochemical pathways with the capacity to accumulate high levels of unusual fatty acids, transcript expression and protein sequences of developing seeds of Physaria fendleri, wild-type Camelina sativa, and Camelina sativa expressing a castor bean (Ricinus communis) hydroxylase were analyzed. A number of potential evolutionary adaptations within lipid metabolism that probably enhance HFA production and accumulation in Physaria fendleri, and, in their absence, limit accumulation in transgenic tissues were revealed. These adaptations occurred in at least 20 genes within several lipid pathways from the onset of fatty acid synthesis and its regulation to the assembly of triacylglycerols. Lipid genes of Physaria fendleri appear to have co-evolved through modulation of transcriptional abundances and alterations within protein sequences. Only a handful of genes showed evidence for sequence adaptation through gene duplication. Collectively, these evolutionary changes probably occurred to minimize deleterious effects of high HFA amounts and/or to enhance accumulation for physiological advantage. These results shed light on the evolution of pathways for novel fatty acid production in seeds, help explain some of the current limitations to accumulation of HFAs in transgenic plants, and may provide improved strategies for future engineering of their production. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/26991237/Identification_of_multiple_lipid_genes_with_modifications_in_expression_and_sequence_associated_with_the_evolution_of_hydroxy_fatty_acid_accumulation_in_Physaria_fendleri_ L2 - https://doi.org/10.1111/tpj.13163 DB - PRIME DP - Unbound Medicine ER -