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Tumour-associated and non-tumour-associated microbiota in colorectal cancer.
Gut. 2017 04; 66(4):633-643.Gut

Abstract

OBJECTIVE

A signature that unifies the colorectal cancer (CRC) microbiota across multiple studies has not been identified. In addition to methodological variance, heterogeneity may be caused by both microbial and host response differences, which was addressed in this study.

DESIGN

We prospectively studied the colonic microbiota and the expression of specific host response genes using faecal and mucosal samples ('ON' and 'OFF' the tumour, proximal and distal) from 59 patients undergoing surgery for CRC, 21 individuals with polyps and 56 healthy controls. Microbiota composition was determined by 16S rRNA amplicon sequencing; expression of host genes involved in CRC progression and immune response was quantified by real-time quantitative PCR.

RESULTS

The microbiota of patients with CRC differed from that of controls, but alterations were not restricted to the cancerous tissue. Differences between distal and proximal cancers were detected and faecal microbiota only partially reflected mucosal microbiota in CRC. Patients with CRC can be stratified based on higher level structures of mucosal-associated bacterial co-abundance groups (CAGs) that resemble the previously formulated concept of enterotypes. Of these, Bacteroidetes Cluster 1 and Firmicutes Cluster 1 were in decreased abundance in CRC mucosa, whereas Bacteroidetes Cluster 2, Firmicutes Cluster 2, Pathogen Cluster and Prevotella Cluster showed increased abundance in CRC mucosa. CRC-associated CAGs were differentially correlated with the expression of host immunoinflammatory response genes.

CONCLUSIONS

CRC-associated microbiota profiles differ from those in healthy subjects and are linked with distinct mucosal gene-expression profiles. Compositional alterations in the microbiota are not restricted to cancerous tissue and differ between distal and proximal cancers.

Authors+Show Affiliations

APC Microbiome Institute, University College Cork, Cork, Ireland. School of Microbiology, University College Cork, Cork, Ireland.APC Microbiome Institute, University College Cork, Cork, Ireland. School of Microbiology, University College Cork, Cork, Ireland.APC Microbiome Institute, University College Cork, Cork, Ireland. School of Microbiology, University College Cork, Cork, Ireland.APC Microbiome Institute, University College Cork, Cork, Ireland. School of Microbiology, University College Cork, Cork, Ireland.APC Microbiome Institute, University College Cork, Cork, Ireland. School of Microbiology, University College Cork, Cork, Ireland.APC Microbiome Institute, University College Cork, Cork, Ireland. School of Microbiology, University College Cork, Cork, Ireland.Mercy University Hospital, Cork, Ireland.APC Microbiome Institute, University College Cork, Cork, Ireland. Department of Medicine, University College Cork, Cork, Ireland.APC Microbiome Institute, University College Cork, Cork, Ireland. School of Microbiology, University College Cork, Cork, Ireland.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26992426

Citation

Flemer, Burkhardt, et al. "Tumour-associated and Non-tumour-associated Microbiota in Colorectal Cancer." Gut, vol. 66, no. 4, 2017, pp. 633-643.
Flemer B, Lynch DB, Brown JM, et al. Tumour-associated and non-tumour-associated microbiota in colorectal cancer. Gut. 2017;66(4):633-643.
Flemer, B., Lynch, D. B., Brown, J. M., Jeffery, I. B., Ryan, F. J., Claesson, M. J., O'Riordain, M., Shanahan, F., & O'Toole, P. W. (2017). Tumour-associated and non-tumour-associated microbiota in colorectal cancer. Gut, 66(4), 633-643. https://doi.org/10.1136/gutjnl-2015-309595
Flemer B, et al. Tumour-associated and Non-tumour-associated Microbiota in Colorectal Cancer. Gut. 2017;66(4):633-643. PubMed PMID: 26992426.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tumour-associated and non-tumour-associated microbiota in colorectal cancer. AU - Flemer,Burkhardt, AU - Lynch,Denise B, AU - Brown,Jillian M R, AU - Jeffery,Ian B, AU - Ryan,Feargal J, AU - Claesson,Marcus J, AU - O'Riordain,Micheal, AU - Shanahan,Fergus, AU - O'Toole,Paul W, Y1 - 2016/03/18/ PY - 2015/03/15/received PY - 2015/12/18/revised PY - 2015/12/21/accepted PY - 2016/3/20/pubmed PY - 2017/7/8/medline PY - 2016/3/20/entrez KW - COLORECTAL CANCER KW - GENE EXPRESSION KW - INTESTINAL MICROBIOLOGY SP - 633 EP - 643 JF - Gut JO - Gut VL - 66 IS - 4 N2 - OBJECTIVE: A signature that unifies the colorectal cancer (CRC) microbiota across multiple studies has not been identified. In addition to methodological variance, heterogeneity may be caused by both microbial and host response differences, which was addressed in this study. DESIGN: We prospectively studied the colonic microbiota and the expression of specific host response genes using faecal and mucosal samples ('ON' and 'OFF' the tumour, proximal and distal) from 59 patients undergoing surgery for CRC, 21 individuals with polyps and 56 healthy controls. Microbiota composition was determined by 16S rRNA amplicon sequencing; expression of host genes involved in CRC progression and immune response was quantified by real-time quantitative PCR. RESULTS: The microbiota of patients with CRC differed from that of controls, but alterations were not restricted to the cancerous tissue. Differences between distal and proximal cancers were detected and faecal microbiota only partially reflected mucosal microbiota in CRC. Patients with CRC can be stratified based on higher level structures of mucosal-associated bacterial co-abundance groups (CAGs) that resemble the previously formulated concept of enterotypes. Of these, Bacteroidetes Cluster 1 and Firmicutes Cluster 1 were in decreased abundance in CRC mucosa, whereas Bacteroidetes Cluster 2, Firmicutes Cluster 2, Pathogen Cluster and Prevotella Cluster showed increased abundance in CRC mucosa. CRC-associated CAGs were differentially correlated with the expression of host immunoinflammatory response genes. CONCLUSIONS: CRC-associated microbiota profiles differ from those in healthy subjects and are linked with distinct mucosal gene-expression profiles. Compositional alterations in the microbiota are not restricted to cancerous tissue and differ between distal and proximal cancers. SN - 1468-3288 UR - https://www.unboundmedicine.com/medline/citation/26992426/Tumour_associated_and_non_tumour_associated_microbiota_in_colorectal_cancer_ L2 - http://gut.bmj.com/lookup/pmidlookup?view=long&pmid=26992426 DB - PRIME DP - Unbound Medicine ER -