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Effects of selenium on ischaemia-reperfusion injury in a rat testis model.
Andrologia. 2016 Dec; 48(10):1267-1273.A

Abstract

Selenium is shown to have beneficial effects on ischaemia-reperfusion (IR) injury. Our aim was to assess the effects of selenium on IR-induced testicular damage in terms of biochemical and histopathological evaluation. A total of 32 rats were randomised into four groups: control, IR, IR + selenium (IR + S) and S. Detorsion was applied after 3 h of torsion. Testicular tissue superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), total antioxidant capacity (TAC) and DNA fragmentation levels were determined. Testicular tissue samples were examined by histopathological examination and terminal deoxynucleotidyl transferase dUTP nick end-labelling staining. The control, IR and IR + S groups had higher SOD values compared with the S group; SOD levels of the control and IR + S groups were higher than those of the IR group (P < 0.05). Further, MDA levels of the IR group were higher than those in the other three groups (P < 0.05). The IR group revealed lower TAC levels than the three groups (P < 0.05 for all). GSH levels of the IR group were significantly lower than those in the other three groups (P < 0.05 for all). In contrast, GSH levels of the IR + S group increased compared with those of the S group. The IR group had more DNA fragmentation than the control and S groups (P < 0.05). It is concluded that selenium possibly reduces oxidative stress and apoptosis caused by testicular IR injury in rats. The testicular protective effect of selenium appears to be mediated through its anti-apoptotic and antioxidative effects. However, selenium does not affect DNA fragmentation.

Authors+Show Affiliations

Department of Urology, Amasya University Faculty of Medicine, Amasya, Turkey.Department of Urology, Balikesir University Faculty of Medicine, Balikesir, Turkey.Department of Biochemistry, Balikesir University Faculty of Veterinary, Balikesir, Turkey.Department of Histology and Embriyology, Erciyes University Faculty of Medicine, Kayseri, Turkey.Department of Pharmacology, Balikesir University Faculty of Veterinary, Balikesir, Turkey.Department of Urology, İstanbul University İstanbul Faculty of Medicine, İstanbul, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26992892

Citation

Kara, Ö, et al. "Effects of Selenium On Ischaemia-reperfusion Injury in a Rat Testis Model." Andrologia, vol. 48, no. 10, 2016, pp. 1267-1273.
Kara Ö, Sari E, Akşit H, et al. Effects of selenium on ischaemia-reperfusion injury in a rat testis model. Andrologia. 2016;48(10):1267-1273.
Kara, Ö., Sari, E., Akşit, H., Yay, A., Akşit, D., & Dönmez, M. I. (2016). Effects of selenium on ischaemia-reperfusion injury in a rat testis model. Andrologia, 48(10), 1267-1273. https://doi.org/10.1111/and.12571
Kara Ö, et al. Effects of Selenium On Ischaemia-reperfusion Injury in a Rat Testis Model. Andrologia. 2016;48(10):1267-1273. PubMed PMID: 26992892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of selenium on ischaemia-reperfusion injury in a rat testis model. AU - Kara,Ö, AU - Sari,E, AU - Akşit,H, AU - Yay,A, AU - Akşit,D, AU - Dönmez,M I, Y1 - 2016/03/18/ PY - 2016/01/28/accepted PY - 2016/3/20/pubmed PY - 2017/2/10/medline PY - 2016/3/20/entrez KW - Animal model KW - ischaemia KW - reperfusion injury KW - selenium KW - testicular torsion SP - 1267 EP - 1273 JF - Andrologia JO - Andrologia VL - 48 IS - 10 N2 - Selenium is shown to have beneficial effects on ischaemia-reperfusion (IR) injury. Our aim was to assess the effects of selenium on IR-induced testicular damage in terms of biochemical and histopathological evaluation. A total of 32 rats were randomised into four groups: control, IR, IR + selenium (IR + S) and S. Detorsion was applied after 3 h of torsion. Testicular tissue superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), total antioxidant capacity (TAC) and DNA fragmentation levels were determined. Testicular tissue samples were examined by histopathological examination and terminal deoxynucleotidyl transferase dUTP nick end-labelling staining. The control, IR and IR + S groups had higher SOD values compared with the S group; SOD levels of the control and IR + S groups were higher than those of the IR group (P < 0.05). Further, MDA levels of the IR group were higher than those in the other three groups (P < 0.05). The IR group revealed lower TAC levels than the three groups (P < 0.05 for all). GSH levels of the IR group were significantly lower than those in the other three groups (P < 0.05 for all). In contrast, GSH levels of the IR + S group increased compared with those of the S group. The IR group had more DNA fragmentation than the control and S groups (P < 0.05). It is concluded that selenium possibly reduces oxidative stress and apoptosis caused by testicular IR injury in rats. The testicular protective effect of selenium appears to be mediated through its anti-apoptotic and antioxidative effects. However, selenium does not affect DNA fragmentation. SN - 1439-0272 UR - https://www.unboundmedicine.com/medline/citation/26992892/Effects_of_selenium_on_ischaemia_reperfusion_injury_in_a_rat_testis_model_ DB - PRIME DP - Unbound Medicine ER -