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Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron.
Aquat Toxicol. 2016 Jun; 175:47-55.AT

Abstract

The Pacific oyster Crassostrea gigas is the main oyster species produced in the world, and a key coastal economic resource in France. High mortalities affect Pacific oysters since 2008 in France and Europe. Their origins have been attributed to a combination of biotic and abiotic factors, underlining the importance of environment quality. The impact of water pollution has been pointed out and one of the pollutants, the genotoxic herbicide diuron, occurs at high concentrations all along the French coasts. Previous work has revealed that a parental exposure to diuron had a strong impact on hatching rates and offspring development even if spats were not exposed to diuron themselves. In this study, we explored for the first time the transcriptional changes occurring in oyster spats (non exposed) originating from genitors exposed to an environmentally relevant concentration of diuron during gametogenesis using the RNAseq methodology. We identified a transcriptomic remodeling revealing an effect of the herbicide. Different molecular pathways involved in energy production, translation and cell proliferation are particularly disturbed. This analysis revealed modulated candidate genes putatively involved in response to oxidative stress and mitochondrial damage in offspring of genitors exposed to diuron. Complementary measures of the activity of enzymes involved in these latter processes corroborate the results obtained at the transcriptomic level. In addition, our results suggested an increase in energy production and mitotic activity in 5-month-spats from diuron-exposed genitors. These results could correspond to a "catch-up growth" phenomenon allowing the spats from diuron-exposed genitors, which displayed a growth delay at 3 months, to gain a normal size when they reach the age of 6 months. These results indicate that exposure to a concentration of diuron that is frequently encountered in the field during the oyster's gametogenesis stage can impact the next generation and may result in fitness disturbance.

Authors+Show Affiliations

Ifremer, IHPE UMR 5244, Univ. Perpignan Via Domitia, CNRS, Univ. Montpellier, F-34095 Montpellier, France; Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860 Perpignan, France.Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France.CNRS, IHPE UMR 5244, Univ. Perpignan Via Domitia, IFREMER, Univ. Montpellier, F-34095 Montpellier, France.Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France.Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France.Ifremer, IHPE UMR 5244, Univ. Perpignan Via Domitia, CNRS, Univ. Montpellier, F-34095 Montpellier, France. Electronic address: cmontagn@ifremer.fr.Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860 Perpignan, France.Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860 Perpignan, France.Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860 Perpignan, France.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26994368

Citation

Rondon, R, et al. "Transcriptional Changes in Crassostrea Gigas Oyster Spat Following a Parental Exposure to the Herbicide Diuron." Aquatic Toxicology (Amsterdam, Netherlands), vol. 175, 2016, pp. 47-55.
Rondon R, Akcha F, Alonso P, et al. Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron. Aquat Toxicol. 2016;175:47-55.
Rondon, R., Akcha, F., Alonso, P., Menard, D., Rouxel, J., Montagnani, C., Mitta, G., Cosseau, C., & Grunau, C. (2016). Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron. Aquatic Toxicology (Amsterdam, Netherlands), 175, 47-55. https://doi.org/10.1016/j.aquatox.2016.03.007
Rondon R, et al. Transcriptional Changes in Crassostrea Gigas Oyster Spat Following a Parental Exposure to the Herbicide Diuron. Aquat Toxicol. 2016;175:47-55. PubMed PMID: 26994368.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron. AU - Rondon,R, AU - Akcha,F, AU - Alonso,P, AU - Menard,D, AU - Rouxel,J, AU - Montagnani,C, AU - Mitta,G, AU - Cosseau,C, AU - Grunau,C, Y1 - 2016/03/09/ PY - 2015/08/07/received PY - 2016/03/01/revised PY - 2016/03/06/accepted PY - 2016/3/20/entrez PY - 2016/3/20/pubmed PY - 2016/12/20/medline KW - Diuron KW - Ecotoxicology KW - Mollusk KW - Oyster KW - Transcriptomic SP - 47 EP - 55 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 175 N2 - The Pacific oyster Crassostrea gigas is the main oyster species produced in the world, and a key coastal economic resource in France. High mortalities affect Pacific oysters since 2008 in France and Europe. Their origins have been attributed to a combination of biotic and abiotic factors, underlining the importance of environment quality. The impact of water pollution has been pointed out and one of the pollutants, the genotoxic herbicide diuron, occurs at high concentrations all along the French coasts. Previous work has revealed that a parental exposure to diuron had a strong impact on hatching rates and offspring development even if spats were not exposed to diuron themselves. In this study, we explored for the first time the transcriptional changes occurring in oyster spats (non exposed) originating from genitors exposed to an environmentally relevant concentration of diuron during gametogenesis using the RNAseq methodology. We identified a transcriptomic remodeling revealing an effect of the herbicide. Different molecular pathways involved in energy production, translation and cell proliferation are particularly disturbed. This analysis revealed modulated candidate genes putatively involved in response to oxidative stress and mitochondrial damage in offspring of genitors exposed to diuron. Complementary measures of the activity of enzymes involved in these latter processes corroborate the results obtained at the transcriptomic level. In addition, our results suggested an increase in energy production and mitotic activity in 5-month-spats from diuron-exposed genitors. These results could correspond to a "catch-up growth" phenomenon allowing the spats from diuron-exposed genitors, which displayed a growth delay at 3 months, to gain a normal size when they reach the age of 6 months. These results indicate that exposure to a concentration of diuron that is frequently encountered in the field during the oyster's gametogenesis stage can impact the next generation and may result in fitness disturbance. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/26994368/Transcriptional_changes_in_Crassostrea_gigas_oyster_spat_following_a_parental_exposure_to_the_herbicide_diuron_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(16)30060-1 DB - PRIME DP - Unbound Medicine ER -