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[Does the sampling locality influence on the antifungal activity of the flavonoids of Marrubium vulgare against Aspergillus niger and Candida albicans?].
J Mycol Med. 2016 Sep; 26(3):201-11.JM

Abstract

OBJECTIVE

The study was undertaken to determine the effect of the sampling locality on the antifungal activity of the flavonoids extracted from the leaves of Marrubium vulgare L. against two fungal strains; Aspergillus niger ATCC 16404 and Candida albicans ATCC 10231.

MATERIALS AND METHODS

The leaves were collected from three different sampling localities belonging northwest Algeria: Tessala mount, M'sila forest and Ain Skhouna. The flavonoid extraction was carried out by using organic solvents with increasing polarity. A phytochemical screening was performed by staining test tubes. The inhibition diameters were measured by solid medium diffusion method. The minimum inhibitory concentrations were determined by dilution method on solid medium.

RESULTS

The antifungal activity varied significantly (P<0.001) according to the sampling locality of the leaves, the flavonoid extract and its concentration, and the strain fungal type. The inhibition diameters varied between 8.16 and recorded 37.5mm even recording a total inhibition of fungal growth and often exceed those induced marketed antifungals (Amphotericin, Fluconazole, Terbinafine and econazole nitrate). The minimum inhibitory concentrations (MICs) obtained range between 6.25 and 100μg/mL; experiencing strong antifungal inhibition. The phytochemical screening revealed the existence of certain flavonoids classes such as flavans and flavanols which may be responsible of this remarkable antifungal power.

CONCLUSION

The sampling locality of Marrubium vulgare leaves influenced on the antifungal activity of flavonoids. These have proven very good fungistatic and worth valuing in pharmacology.

Authors+Show Affiliations

Laboratoire de biodiversité végétale : conservation et valorisation, faculté des sciences de la nature et de la vie, université Djillali Liabes, BP 89, Haï Larbi Ben M'hidi, 22000 Sidi Bel-Abbés, Algérie; Département de l'environnement, faculté des sciences de la nature et de la vie, université Djillali Liabes, 22000 Sidi Bel-Abbés, Algérie. Electronic address: bouterfas_karim@yahoo.fr.Laboratoire de biodiversité végétale : conservation et valorisation, faculté des sciences de la nature et de la vie, université Djillali Liabes, BP 89, Haï Larbi Ben M'hidi, 22000 Sidi Bel-Abbés, Algérie; Département de l'environnement, faculté des sciences de la nature et de la vie, université Djillali Liabes, 22000 Sidi Bel-Abbés, Algérie.Faculté de médecine, université Djillali Liabès, 22000 Sidi Bel-Abbés, Algérie.Laboratoire de biotoxicologie, faculté des sciences de la nature et de la vie, université Djillali Liabes, 22000 Sidi Bel-Abbés, Algérie.Laboratoire de biotoxicologie, faculté des sciences de la nature et de la vie, université Djillali Liabes, 22000 Sidi Bel-Abbés, Algérie.Laboratoire de biodiversité végétale : conservation et valorisation, faculté des sciences de la nature et de la vie, université Djillali Liabes, BP 89, Haï Larbi Ben M'hidi, 22000 Sidi Bel-Abbés, Algérie; Département de l'environnement, faculté des sciences de la nature et de la vie, université Djillali Liabes, 22000 Sidi Bel-Abbés, Algérie.Laboratoire de biodiversité végétale : conservation et valorisation, faculté des sciences de la nature et de la vie, université Djillali Liabes, BP 89, Haï Larbi Ben M'hidi, 22000 Sidi Bel-Abbés, Algérie; Département de l'environnement, faculté des sciences de la nature et de la vie, université Djillali Liabes, 22000 Sidi Bel-Abbés, Algérie.

Pub Type(s)

Journal Article

Language

fre

PubMed ID

26994761

Citation

Bouterfas, K, et al. "[Does the Sampling Locality Influence On the Antifungal Activity of the Flavonoids of Marrubium Vulgare Against Aspergillus Niger and Candida Albicans?]." Journal De Mycologie Medicale, vol. 26, no. 3, 2016, pp. 201-11.
Bouterfas K, Mehdadi Z, Aouad L, et al. [Does the sampling locality influence on the antifungal activity of the flavonoids of Marrubium vulgare against Aspergillus niger and Candida albicans?]. J Mycol Med. 2016;26(3):201-11.
Bouterfas, K., Mehdadi, Z., Aouad, L., Elaoufi, M. M., Khaled, M. B., Latreche, A., & Benchiha, W. (2016). [Does the sampling locality influence on the antifungal activity of the flavonoids of Marrubium vulgare against Aspergillus niger and Candida albicans?]. Journal De Mycologie Medicale, 26(3), 201-11. https://doi.org/10.1016/j.mycmed.2016.02.019
Bouterfas K, et al. [Does the Sampling Locality Influence On the Antifungal Activity of the Flavonoids of Marrubium Vulgare Against Aspergillus Niger and Candida Albicans?]. J Mycol Med. 2016;26(3):201-11. PubMed PMID: 26994761.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Does the sampling locality influence on the antifungal activity of the flavonoids of Marrubium vulgare against Aspergillus niger and Candida albicans?]. AU - Bouterfas,K, AU - Mehdadi,Z, AU - Aouad,L, AU - Elaoufi,M M, AU - Khaled,M B, AU - Latreche,A, AU - Benchiha,W, Y1 - 2016/03/17/ PY - 2015/11/17/received PY - 2016/02/05/revised PY - 2016/02/13/accepted PY - 2016/3/21/entrez PY - 2016/3/21/pubmed PY - 2017/9/8/medline KW - Activité antifongique KW - Antifungal activity KW - Flavonoids KW - Flavonoïdes KW - Localité d’échantillonnage KW - Marrubium vulgare KW - Sampling locality SP - 201 EP - 11 JF - Journal de mycologie medicale JO - J Mycol Med VL - 26 IS - 3 N2 - OBJECTIVE: The study was undertaken to determine the effect of the sampling locality on the antifungal activity of the flavonoids extracted from the leaves of Marrubium vulgare L. against two fungal strains; Aspergillus niger ATCC 16404 and Candida albicans ATCC 10231. MATERIALS AND METHODS: The leaves were collected from three different sampling localities belonging northwest Algeria: Tessala mount, M'sila forest and Ain Skhouna. The flavonoid extraction was carried out by using organic solvents with increasing polarity. A phytochemical screening was performed by staining test tubes. The inhibition diameters were measured by solid medium diffusion method. The minimum inhibitory concentrations were determined by dilution method on solid medium. RESULTS: The antifungal activity varied significantly (P<0.001) according to the sampling locality of the leaves, the flavonoid extract and its concentration, and the strain fungal type. The inhibition diameters varied between 8.16 and recorded 37.5mm even recording a total inhibition of fungal growth and often exceed those induced marketed antifungals (Amphotericin, Fluconazole, Terbinafine and econazole nitrate). The minimum inhibitory concentrations (MICs) obtained range between 6.25 and 100μg/mL; experiencing strong antifungal inhibition. The phytochemical screening revealed the existence of certain flavonoids classes such as flavans and flavanols which may be responsible of this remarkable antifungal power. CONCLUSION: The sampling locality of Marrubium vulgare leaves influenced on the antifungal activity of flavonoids. These have proven very good fungistatic and worth valuing in pharmacology. SN - 1773-0449 UR - https://www.unboundmedicine.com/medline/citation/26994761/[Does_the_sampling_locality_influence_on_the_antifungal_activity_of_the_flavonoids_of_Marrubium_vulgare_against_Aspergillus_niger_and_Candida_albicans]_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1156-5233(16)00030-5 DB - PRIME DP - Unbound Medicine ER -