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[Isolation and characteristics of a recombinant pOV13 plasmid as a vector for DNA cloning in a broad range of bacterial hosts].
Mol Gen Mikrobiol Virusol. 1989 DecMG

Abstract

The hybrid plasmid pOV13 proposed as a potential vector for DNA cloning in a broad bacterial host range has been constructed on the basis of the broad host range plasmid RSF1010 and a shortened derivative of RP4, the plasmid pVZ115 serving a marker DNA fragment. The plasmid pOV13 contains the genes for streptomycin, kanamycin and tetracycline resistance and single cleavage sites for restriction endonucleases BamHI, BgIII, SalI, SmaI, PvuII, XhoI, as well as double cleavage sites for restriction endonucleases PstI and HindIII permitting one to clone DNA with insertional inactivation of genes. The physicogenetical map of the birepliconed plasmid pOV13 is presented.

Authors

Zakharenko VI
No affiliation info available
Gorelov VN
No affiliation info available
Nenashev AV
No affiliation info available
Skavronskaia AG
No affiliation info available

MeSH

Cloning, MolecularDNA, BacterialEscherichia coliGenetic VectorsNucleic Acid HybridizationPlasmidsRecombination, GeneticRestriction MappingTransformation, Bacterial

Pub Type(s)

Journal Article

Language

rus

PubMed ID

2699520

Citation

Zakharenko, V I., et al. "[Isolation and Characteristics of a Recombinant pOV13 Plasmid as a Vector for DNA Cloning in a Broad Range of Bacterial Hosts]." Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, 1989, pp. 33-8.
Zakharenko VI, Gorelov VN, Nenashev AV, et al. [Isolation and characteristics of a recombinant pOV13 plasmid as a vector for DNA cloning in a broad range of bacterial hosts]. Mol Gen Mikrobiol Virusol. 1989.
Zakharenko, V. I., Gorelov, V. N., Nenashev, A. V., & Skavronskaia, A. G. (1989). [Isolation and characteristics of a recombinant pOV13 plasmid as a vector for DNA cloning in a broad range of bacterial hosts]. Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, (12), 33-8.
Zakharenko VI, et al. [Isolation and Characteristics of a Recombinant pOV13 Plasmid as a Vector for DNA Cloning in a Broad Range of Bacterial Hosts]. Mol Gen Mikrobiol Virusol. 1989;(12)33-8. PubMed PMID: 2699520.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Isolation and characteristics of a recombinant pOV13 plasmid as a vector for DNA cloning in a broad range of bacterial hosts]. AU - Zakharenko,V I, AU - Gorelov,V N, AU - Nenashev,A V, AU - Skavronskaia,A G, PY - 1989/12/1/pubmed PY - 1989/12/1/medline PY - 1989/12/1/entrez SP - 33 EP - 8 JF - Molekuliarnaia genetika, mikrobiologiia i virusologiia JO - Mol Gen Mikrobiol Virusol IS - 12 N2 - The hybrid plasmid pOV13 proposed as a potential vector for DNA cloning in a broad bacterial host range has been constructed on the basis of the broad host range plasmid RSF1010 and a shortened derivative of RP4, the plasmid pVZ115 serving a marker DNA fragment. The plasmid pOV13 contains the genes for streptomycin, kanamycin and tetracycline resistance and single cleavage sites for restriction endonucleases BamHI, BgIII, SalI, SmaI, PvuII, XhoI, as well as double cleavage sites for restriction endonucleases PstI and HindIII permitting one to clone DNA with insertional inactivation of genes. The physicogenetical map of the birepliconed plasmid pOV13 is presented. SN - 0208-0613 UR - https://www.unboundmedicine.com/medline/citation/2699520/[Isolation_and_characteristics_of_a_recombinant_pOV13_plasmid_as_a_vector_for_DNA_cloning_in_a_broad_range_of_bacterial_hosts]_ DB - PRIME DP - Unbound Medicine ER -