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Venom from the spider Araneus ventricosus is lethal to insects but inactive in vertebrates.
Toxicon. 2016 Jun 01; 115:63-9.T

Abstract

Araneus ventricosus spider venom, which was collected by electrical stimulation, is abundant in peptides and proteins with molecular weights ranging from 2 kDa to 70 kDa as determined by gel electrophoresis and mass spectrometry. Electrophysiological experiments showed that 50 μg/mL venom could block the voltage-gated sodium channels (VGSCs) currents of the dorsal unpaired median (DUM) neurons of Periplaneta americana cockroaches. However, 500 μg/mL venom could not block the VGSCs currents in rat dorsal root ganglion cells or the neuromuscular transmission in isolated mouse phrenic nerve-hemidiaphragm. Moreover, we also observed that injection of the venom in P. americana gave rise to obvious envenomation symptoms, with a LD50 value of 30.7 μg/g. Enzymatic analysis indicated that the venom possessed activities of several kinds of hydrolases including hyaluronidase and proteases. These results demonstrate that A. ventricosus venom contains bioactive components targeting insects, which are the natural prey of these spiders. Furthermore, the venom was found to be not active in vertebrate. Thus, we suggest that A. ventricosus venom contains novel insect-selective compounds that might be helpful in developing new and safe insecticides.

Authors+Show Affiliations

The Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.The Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Qld 4072, Australia.The Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.The Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, Hunan 410081, China.The Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China; National Experiment Teaching Demonstration Center of General Biology, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China. Electronic address: zhiguiduan@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26995209

Citation

Liu, Kai, et al. "Venom From the Spider Araneus Ventricosus Is Lethal to Insects but Inactive in Vertebrates." Toxicon : Official Journal of the International Society On Toxinology, vol. 115, 2016, pp. 63-9.
Liu K, Wang M, Herzig V, et al. Venom from the spider Araneus ventricosus is lethal to insects but inactive in vertebrates. Toxicon. 2016;115:63-9.
Liu, K., Wang, M., Herzig, V., Liu, Z., Hu, W., Zhou, G., & Duan, Z. (2016). Venom from the spider Araneus ventricosus is lethal to insects but inactive in vertebrates. Toxicon : Official Journal of the International Society On Toxinology, 115, 63-9. https://doi.org/10.1016/j.toxicon.2016.03.010
Liu K, et al. Venom From the Spider Araneus Ventricosus Is Lethal to Insects but Inactive in Vertebrates. Toxicon. 2016 Jun 1;115:63-9. PubMed PMID: 26995209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Venom from the spider Araneus ventricosus is lethal to insects but inactive in vertebrates. AU - Liu,Kai, AU - Wang,Meichi, AU - Herzig,Volker, AU - Liu,Zhen, AU - Hu,Weijun, AU - Zhou,Guihua, AU - Duan,Zhigui, Y1 - 2016/03/16/ PY - 2015/12/03/received PY - 2016/02/26/revised PY - 2016/03/15/accepted PY - 2016/3/21/entrez PY - 2016/3/21/pubmed PY - 2017/1/31/medline KW - Araneus ventricousus KW - Electrophysiological analysis KW - Venom composition KW - Voltage-gated sodium channels (VGSCs) SP - 63 EP - 9 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 115 N2 - Araneus ventricosus spider venom, which was collected by electrical stimulation, is abundant in peptides and proteins with molecular weights ranging from 2 kDa to 70 kDa as determined by gel electrophoresis and mass spectrometry. Electrophysiological experiments showed that 50 μg/mL venom could block the voltage-gated sodium channels (VGSCs) currents of the dorsal unpaired median (DUM) neurons of Periplaneta americana cockroaches. However, 500 μg/mL venom could not block the VGSCs currents in rat dorsal root ganglion cells or the neuromuscular transmission in isolated mouse phrenic nerve-hemidiaphragm. Moreover, we also observed that injection of the venom in P. americana gave rise to obvious envenomation symptoms, with a LD50 value of 30.7 μg/g. Enzymatic analysis indicated that the venom possessed activities of several kinds of hydrolases including hyaluronidase and proteases. These results demonstrate that A. ventricosus venom contains bioactive components targeting insects, which are the natural prey of these spiders. Furthermore, the venom was found to be not active in vertebrate. Thus, we suggest that A. ventricosus venom contains novel insect-selective compounds that might be helpful in developing new and safe insecticides. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/26995209/Venom_from_the_spider_Araneus_ventricosus_is_lethal_to_insects_but_inactive_in_vertebrates_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(16)30050-2 DB - PRIME DP - Unbound Medicine ER -