Tags

Type your tag names separated by a space and hit enter

Study of Silymarin and Vitamin E Protective Effects on Silver Nanoparticle Toxicity on Mice Liver Primary Cell Culture.
Acta Med Iran. 2016 Feb; 54(2):85-95.AM

Abstract

Nanotechnology is a most promising field for generating new applications in medicine, although, only few nano products are currently in use for medical purposes. A most prominent nanoproduct is nanosilver. Nano-silver has biological properties which are significant for consumer products, food technology, textiles, and medical applications (e.g. wound care products, implantable medical devices, in diagnosis, drug delivery, and imaging). For their antibacterial activity, silver nanoparticles (Ag NPs) are largely used in various commercially available products. The use of nano-silver is becoming more and more widespread in medicine and related applications, and due to its increasing exposure, toxicological and environmental issues need to be raised. Cytotoxicity induced by silver nanoparticles (AgNPs) and the role that oxidative stress plays in this process were demonstrated in human hepatoma cells AgNPs agglomerated in the cytoplasm and nuclei of treated cells, and they induced intracellular oxidative stress. AgNP reduced ATP content of the cell and caused damage to mitochondria and increased production of reactive oxygen species (ROS) in a dose-dependent manner. Silymarin was known as a hepatoprotective agent that is used in the treatment of hepatic diseases including viral hepatitis, alcoholic liver diseases, Amanita mushroom poisoning, liver cirrhosis, toxic and drug-induced liver diseases. It promotes protein synthesis, helps in regenerating liver tissue, controls inflammation, enhances glucuronidation, and protects against glutathione depletion. Vitamin E is a well-known antioxidant and has hepatoprotective effect in liver diseases. In this study, we investigated the cytotoxic effects of Ag NPs on primary liver cells of mice. Cell viability (cytotoxicity) was examined with MTT assay after primary liver cells of mice exposure to AgNPs at 1, 10, 50, 100, 150, 200, 400 ppm for 24h. AgNPs caused a concentration- dependent decrease of cell viability (IC50 value = 121.7 ppm or µg/ml). Then the hepatoprotective effect of silymarin and vitamin E were experimented on silver nanoparticle toxicity on mice liver primary cell culture. The results showed that silymarin at 600 µg/ml and vitamin E at 2500 µmol/l have protective effects on silver nanoparticle toxicity on mice liver primary cell culture. Viability percentage of the primary liver cell of the mouse were exposed to silver nanoparticles at 121.7 ppm and co-treatment of silymarin, and vitamin E is more than viability percentage of the primary liver cell of the mouse were exposed to silver nanoparticles and silymarin or silver nanoparticles and vitamin E.

Authors+Show Affiliations

Department of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Pharmacology, Tehran University of Medical Sciences, Tehran, Iran.Health Reference laboratories, Ministry of Health and Medical Education, Tehran, Iran.Deptartment of Toxicology, Aja University of Medical Science, Tehran, Iran.Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran. AND Cosmetic Products Research Center, Iran Food and Drug Adminstration, Ministry of Health and Medical Education, Tehran, Iran.Department of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. AND Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran. AND Cosmetic Products Research Center, Iran Food and Drug Adminstration, Ministry of Health and Medical Education, Tehran, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26997594

Citation

Faedmaleki, Firouz, et al. "Study of Silymarin and Vitamin E Protective Effects On Silver Nanoparticle Toxicity On Mice Liver Primary Cell Culture." Acta Medica Iranica, vol. 54, no. 2, 2016, pp. 85-95.
Faedmaleki F, Shirazi FH, Ejtemaeimehr S, et al. Study of Silymarin and Vitamin E Protective Effects on Silver Nanoparticle Toxicity on Mice Liver Primary Cell Culture. Acta Med Iran. 2016;54(2):85-95.
Faedmaleki, F., Shirazi, F. H., Ejtemaeimehr, S., Anjarani, S., Salarian, A. A., Ahmadi Ashtiani, H., & Rastegar, H. (2016). Study of Silymarin and Vitamin E Protective Effects on Silver Nanoparticle Toxicity on Mice Liver Primary Cell Culture. Acta Medica Iranica, 54(2), 85-95.
Faedmaleki F, et al. Study of Silymarin and Vitamin E Protective Effects On Silver Nanoparticle Toxicity On Mice Liver Primary Cell Culture. Acta Med Iran. 2016;54(2):85-95. PubMed PMID: 26997594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Study of Silymarin and Vitamin E Protective Effects on Silver Nanoparticle Toxicity on Mice Liver Primary Cell Culture. AU - Faedmaleki,Firouz, AU - Shirazi,Farshad H, AU - Ejtemaeimehr,Shahram, AU - Anjarani,Soghra, AU - Salarian,Amir-Ahmad, AU - Ahmadi Ashtiani,Hamidreza, AU - Rastegar,Hossein, PY - 2016/3/22/entrez PY - 2016/3/22/pubmed PY - 2016/11/4/medline KW - MTT assay KW - Silver nanoparticle KW - Silymarin KW - Vitamin E SP - 85 EP - 95 JF - Acta medica Iranica JO - Acta Med Iran VL - 54 IS - 2 N2 - Nanotechnology is a most promising field for generating new applications in medicine, although, only few nano products are currently in use for medical purposes. A most prominent nanoproduct is nanosilver. Nano-silver has biological properties which are significant for consumer products, food technology, textiles, and medical applications (e.g. wound care products, implantable medical devices, in diagnosis, drug delivery, and imaging). For their antibacterial activity, silver nanoparticles (Ag NPs) are largely used in various commercially available products. The use of nano-silver is becoming more and more widespread in medicine and related applications, and due to its increasing exposure, toxicological and environmental issues need to be raised. Cytotoxicity induced by silver nanoparticles (AgNPs) and the role that oxidative stress plays in this process were demonstrated in human hepatoma cells AgNPs agglomerated in the cytoplasm and nuclei of treated cells, and they induced intracellular oxidative stress. AgNP reduced ATP content of the cell and caused damage to mitochondria and increased production of reactive oxygen species (ROS) in a dose-dependent manner. Silymarin was known as a hepatoprotective agent that is used in the treatment of hepatic diseases including viral hepatitis, alcoholic liver diseases, Amanita mushroom poisoning, liver cirrhosis, toxic and drug-induced liver diseases. It promotes protein synthesis, helps in regenerating liver tissue, controls inflammation, enhances glucuronidation, and protects against glutathione depletion. Vitamin E is a well-known antioxidant and has hepatoprotective effect in liver diseases. In this study, we investigated the cytotoxic effects of Ag NPs on primary liver cells of mice. Cell viability (cytotoxicity) was examined with MTT assay after primary liver cells of mice exposure to AgNPs at 1, 10, 50, 100, 150, 200, 400 ppm for 24h. AgNPs caused a concentration- dependent decrease of cell viability (IC50 value = 121.7 ppm or µg/ml). Then the hepatoprotective effect of silymarin and vitamin E were experimented on silver nanoparticle toxicity on mice liver primary cell culture. The results showed that silymarin at 600 µg/ml and vitamin E at 2500 µmol/l have protective effects on silver nanoparticle toxicity on mice liver primary cell culture. Viability percentage of the primary liver cell of the mouse were exposed to silver nanoparticles at 121.7 ppm and co-treatment of silymarin, and vitamin E is more than viability percentage of the primary liver cell of the mouse were exposed to silver nanoparticles and silymarin or silver nanoparticles and vitamin E. SN - 1735-9694 UR - https://www.unboundmedicine.com/medline/citation/26997594/Study_of_Silymarin_and_Vitamin_E_Protective_Effects_on_Silver_Nanoparticle_Toxicity_on_Mice_Liver_Primary_Cell_Culture_ L2 - http://acta.tums.ac.ir/index.php/acta/article/view/5493 DB - PRIME DP - Unbound Medicine ER -