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Pregnancy prediction by free sperm DNA and sperm DNA fragmentation in semen specimens of IVF/ICSI-ET patients.
Hum Fertil (Camb) 2016; 19(1):56-62HF

Abstract

The purpose of this study was to evaluate the predictive value of free sperm plasma DNA (f-spDNA) and sperm DNA fragmentation (SDF), in semen specimens from men undergoing in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) treatments. Fifty-five semen samples were evaluated during 55 consecutive IVF/ICSI-ET cycles. F-spDNA was determined by conventional quantitative real-time PCR-Sybr green detection approach, while evaluation of sperm DNA damage was performed using the sperm chromatin dispersion (SCD) assay. While f-spDNA only correlated with total sperm count, SDF correlated with many semen parameters (including sperm concentration, total sperm count and the per cent of non-progressive sperm). Neither SDF nor the proportion of sperm with small or no halos correlated with f-spDNA. Interestingly, smoking status correlated with f-spDNA but not with SDF. Although these two factors seem to interact for the prediction of pregnancy, receiver-operating characteristics (ROC) analysis revealed that SDF had a stronger predictive value (AUC = 0.7, p < 0.05) than f-spDNA (AUC = 0.6, p > 0.05). SDF and f-spDNA may not be associated together but they interact at a significant level in order to exert their actions on pregnancy outcome. Among the two markers, SDF appears to have stronger and significantly predictive value for pregnancy success.

Authors+Show Affiliations

a Department of Obstetrics and Gynecology, School of Health Sciences, Faculty of Medicine Larisa , University of Thessalia , Larisa , Greece ;a Department of Obstetrics and Gynecology, School of Health Sciences, Faculty of Medicine Larisa , University of Thessalia , Larisa , Greece ;a Department of Obstetrics and Gynecology, School of Health Sciences, Faculty of Medicine Larisa , University of Thessalia , Larisa , Greece ;a Department of Obstetrics and Gynecology, School of Health Sciences, Faculty of Medicine Larisa , University of Thessalia , Larisa , Greece ;a Department of Obstetrics and Gynecology, School of Health Sciences, Faculty of Medicine Larisa , University of Thessalia , Larisa , Greece ;b Department of Molecular Biology, School of Health Sciences, Faculty of Medicine Larisa , University of Thessalia , Larisa , Greece.b Department of Molecular Biology, School of Health Sciences, Faculty of Medicine Larisa , University of Thessalia , Larisa , Greece.a Department of Obstetrics and Gynecology, School of Health Sciences, Faculty of Medicine Larisa , University of Thessalia , Larisa , Greece ;b Department of Molecular Biology, School of Health Sciences, Faculty of Medicine Larisa , University of Thessalia , Larisa , Greece.a Department of Obstetrics and Gynecology, School of Health Sciences, Faculty of Medicine Larisa , University of Thessalia , Larisa , Greece ;

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27006263

Citation

Bounartzi, Theofania, et al. "Pregnancy Prediction By Free Sperm DNA and Sperm DNA Fragmentation in Semen Specimens of IVF/ICSI-ET Patients." Human Fertility (Cambridge, England), vol. 19, no. 1, 2016, pp. 56-62.
Bounartzi T, Dafopoulos K, Anifandis G, et al. Pregnancy prediction by free sperm DNA and sperm DNA fragmentation in semen specimens of IVF/ICSI-ET patients. Hum Fertil (Camb). 2016;19(1):56-62.
Bounartzi, T., Dafopoulos, K., Anifandis, G., Messini, C. I., Koutsonikou, C., Kouris, S., ... Messinis, I. E. (2016). Pregnancy prediction by free sperm DNA and sperm DNA fragmentation in semen specimens of IVF/ICSI-ET patients. Human Fertility (Cambridge, England), 19(1), pp. 56-62. doi:10.3109/14647273.2016.1157629.
Bounartzi T, et al. Pregnancy Prediction By Free Sperm DNA and Sperm DNA Fragmentation in Semen Specimens of IVF/ICSI-ET Patients. Hum Fertil (Camb). 2016;19(1):56-62. PubMed PMID: 27006263.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pregnancy prediction by free sperm DNA and sperm DNA fragmentation in semen specimens of IVF/ICSI-ET patients. AU - Bounartzi,Theofania, AU - Dafopoulos,Konstantinos, AU - Anifandis,George, AU - Messini,Christina I, AU - Koutsonikou,Chrysoula, AU - Kouris,Spyros, AU - Satra,Maria, AU - Sotiriou,Sotirios, AU - Vamvakopoulos,Nicholas, AU - Messinis,Ioannis E, Y1 - 2016/03/23/ PY - 2016/3/24/entrez PY - 2016/3/24/pubmed PY - 2017/4/18/medline KW - Free sperm plasma DNA KW - IVF outcome KW - embryo quality KW - semen parameters KW - sperm DNA fragmentation SP - 56 EP - 62 JF - Human fertility (Cambridge, England) JO - Hum Fertil (Camb) VL - 19 IS - 1 N2 - The purpose of this study was to evaluate the predictive value of free sperm plasma DNA (f-spDNA) and sperm DNA fragmentation (SDF), in semen specimens from men undergoing in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) treatments. Fifty-five semen samples were evaluated during 55 consecutive IVF/ICSI-ET cycles. F-spDNA was determined by conventional quantitative real-time PCR-Sybr green detection approach, while evaluation of sperm DNA damage was performed using the sperm chromatin dispersion (SCD) assay. While f-spDNA only correlated with total sperm count, SDF correlated with many semen parameters (including sperm concentration, total sperm count and the per cent of non-progressive sperm). Neither SDF nor the proportion of sperm with small or no halos correlated with f-spDNA. Interestingly, smoking status correlated with f-spDNA but not with SDF. Although these two factors seem to interact for the prediction of pregnancy, receiver-operating characteristics (ROC) analysis revealed that SDF had a stronger predictive value (AUC = 0.7, p < 0.05) than f-spDNA (AUC = 0.6, p > 0.05). SDF and f-spDNA may not be associated together but they interact at a significant level in order to exert their actions on pregnancy outcome. Among the two markers, SDF appears to have stronger and significantly predictive value for pregnancy success. SN - 1742-8149 UR - https://www.unboundmedicine.com/medline/citation/27006263/Pregnancy_prediction_by_free_sperm_DNA_and_sperm_DNA_fragmentation_in_semen_specimens_of_IVF/ICSI_ET_patients_ L2 - http://www.tandfonline.com/doi/full/10.3109/14647273.2016.1157629 DB - PRIME DP - Unbound Medicine ER -