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Dietary ω-6/ω-3 Polyunsaturated Fatty Acid Ratios Affect the Homeostasis of Th/Treg Cells in Mice With Dextran Sulfate Sodium-Induced Colitis.
JPEN J Parenter Enteral Nutr. 2017 05; 41(4):647-656.JJ

Abstract

BACKGROUND

This study evaluated the effect of different dietary ω-6/ω-3 polyunsaturated fatty acid (PUFA) ratios on modulating helper T (Th) and regulatory T (Treg) lymphocytes in mice with dextran sulfate sodium (DSS)-induced colitis.

METHODS

There were 3 control and 3 colitis groups. Mice were fed for 24 days with diets with soybean oil (S), a mixture of soybean oil and low fish oil content (LF), or high fish oil content (HF). The ratio of ω-6/ω-3 PUFA in the LF diet was 4:1, and that in the HF diet was 2:1. The control groups drank distilled water while colitis groups were provided 2% DSS in drinking water during days 15-19. All mice drank distilled water from days 20-24 for recovery and were sacrificed on day 25.

RESULTS

Colitis resulted in higher blood Th1, Th2, and Th17 and lower Treg percentages. Also, plasma haptoglobin and proinflammatory chemokines were elevated in colon lavage fluid. Colitic groups with fish oil had lower inflammatory mediators in the plasma and colon lavage fluid. Furthermore, the percentages of blood Th1, Th2, and Th17 cells were lower, whereas Treg cell percentages were higher than those in the soybean oil group. The colitis group with an ω-6/ω-3 PUFA ratio of 2:1 had more pronounced effects than the group with a ratio of 4:1.

CONCLUSIONS

Diets with an ω-6/ω-3 PUFA ratio of 2:1 or 4:1 regulate the Th/Treg balance and attenuate inflammatory mediator production in colitis. Compared with the ω-6/ω-3 PUFA ratio of 4:1, the ratio of 2:1 was more effective in reducing inflammatory reactions in DSS-induced colitis.

Authors+Show Affiliations

1 School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, Taiwan.2 Master Program in Food Safety, College of Nutrition, Taipei Medical University, Taipei, Taiwan.3 Department of Anatomy and Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.4 Department of Nutrition and Health Science, Chinese Culture University, Taipei, Taiwan.1 School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, Taiwan. 2 Master Program in Food Safety, College of Nutrition, Taipei Medical University, Taipei, Taiwan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27006408

Citation

Huang, Cyoung-Huei, et al. "Dietary Ω-6/ω-3 Polyunsaturated Fatty Acid Ratios Affect the Homeostasis of Th/Treg Cells in Mice With Dextran Sulfate Sodium-Induced Colitis." JPEN. Journal of Parenteral and Enteral Nutrition, vol. 41, no. 4, 2017, pp. 647-656.
Huang CH, Hou YC, Pai MH, et al. Dietary ω-6/ω-3 Polyunsaturated Fatty Acid Ratios Affect the Homeostasis of Th/Treg Cells in Mice With Dextran Sulfate Sodium-Induced Colitis. JPEN J Parenter Enteral Nutr. 2017;41(4):647-656.
Huang, C. H., Hou, Y. C., Pai, M. H., Yeh, C. L., & Yeh, S. L. (2017). Dietary ω-6/ω-3 Polyunsaturated Fatty Acid Ratios Affect the Homeostasis of Th/Treg Cells in Mice With Dextran Sulfate Sodium-Induced Colitis. JPEN. Journal of Parenteral and Enteral Nutrition, 41(4), 647-656. https://doi.org/10.1177/0148607116638493
Huang CH, et al. Dietary Ω-6/ω-3 Polyunsaturated Fatty Acid Ratios Affect the Homeostasis of Th/Treg Cells in Mice With Dextran Sulfate Sodium-Induced Colitis. JPEN J Parenter Enteral Nutr. 2017;41(4):647-656. PubMed PMID: 27006408.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary ω-6/ω-3 Polyunsaturated Fatty Acid Ratios Affect the Homeostasis of Th/Treg Cells in Mice With Dextran Sulfate Sodium-Induced Colitis. AU - Huang,Cyoung-Huei, AU - Hou,Yu-Chen, AU - Pai,Man-Hui, AU - Yeh,Chiu-Li, AU - Yeh,Sung-Ling, Y1 - 2016/03/22/ PY - 2016/3/24/pubmed PY - 2018/3/6/medline PY - 2016/3/24/entrez KW - helper T lymphocyte KW - inflammatory bowel disease KW - regulatory T lymphocyte KW - ω-3 polyunsaturated fatty acids SP - 647 EP - 656 JF - JPEN. Journal of parenteral and enteral nutrition JO - JPEN J Parenter Enteral Nutr VL - 41 IS - 4 N2 - BACKGROUND: This study evaluated the effect of different dietary ω-6/ω-3 polyunsaturated fatty acid (PUFA) ratios on modulating helper T (Th) and regulatory T (Treg) lymphocytes in mice with dextran sulfate sodium (DSS)-induced colitis. METHODS: There were 3 control and 3 colitis groups. Mice were fed for 24 days with diets with soybean oil (S), a mixture of soybean oil and low fish oil content (LF), or high fish oil content (HF). The ratio of ω-6/ω-3 PUFA in the LF diet was 4:1, and that in the HF diet was 2:1. The control groups drank distilled water while colitis groups were provided 2% DSS in drinking water during days 15-19. All mice drank distilled water from days 20-24 for recovery and were sacrificed on day 25. RESULTS: Colitis resulted in higher blood Th1, Th2, and Th17 and lower Treg percentages. Also, plasma haptoglobin and proinflammatory chemokines were elevated in colon lavage fluid. Colitic groups with fish oil had lower inflammatory mediators in the plasma and colon lavage fluid. Furthermore, the percentages of blood Th1, Th2, and Th17 cells were lower, whereas Treg cell percentages were higher than those in the soybean oil group. The colitis group with an ω-6/ω-3 PUFA ratio of 2:1 had more pronounced effects than the group with a ratio of 4:1. CONCLUSIONS: Diets with an ω-6/ω-3 PUFA ratio of 2:1 or 4:1 regulate the Th/Treg balance and attenuate inflammatory mediator production in colitis. Compared with the ω-6/ω-3 PUFA ratio of 4:1, the ratio of 2:1 was more effective in reducing inflammatory reactions in DSS-induced colitis. SN - 1941-2444 UR - https://www.unboundmedicine.com/medline/citation/27006408/Dietary_ω_6/ω_3_Polyunsaturated_Fatty_Acid_Ratios_Affect_the_Homeostasis_of_Th/Treg_Cells_in_Mice_With_Dextran_Sulfate_Sodium_Induced_Colitis_ L2 - https://doi.org/10.1177/0148607116638493 DB - PRIME DP - Unbound Medicine ER -