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Modified citrus pectin stops progression of liver fibrosis by inhibiting galectin-3 and inducing apoptosis of stellate cells.
Can J Physiol Pharmacol. 2016 May; 94(5):554-62.CJ

Abstract

Modified citrus pectin (MCP) is a pH modified form of the dietary soluble citrus peel fiber known as pectin. The current study aims at testing its effect on liver fibrosis progression. Rats were injected with CCl4 (1 mL/kg, 40% v/v, i.p., twice a week for 8 weeks). Concurrently, MCP (400 or 1200 mg/kg) was administered daily in drinking water from the first week in groups I and II (prophylactic model) and in the beginning of week 5 in groups III and IV (therapeutic model). Liver function biomarkers (ATL, AST, and ALP), fibrosis markers (laminin and hyaluronic acid), and antioxidant biomarkers (reduced glutathione (GSH) and superoxide dismutase (SOD)) were measured. Stained liver sections were scored for fibrosis and necroinflammation. Additionally, expression of galectin-3 (Gal-3), α-smooth muscle actin (SMA), tissue inhibitor metalloproteinase (TIMP)-1, collagen (Col)1A1, caspase (Cas)-3, and apoptosis related factor (FAS) were assigned. Modified pectin late administration significantly (p < 0.05) decreased malondialdehyde (MDA), TIMP-1, Col1A1, α-SMA, and Gal-3 levels and increased levels of FAS, Cas-3, GSH, and SOD. It also decreased percentage of fibrosis and necroinflammation significantly (p < 0.05). It can be concluded that MCP can attenuate liver fibrosis through an antioxidant effect, inhibition of Gal-3 mediated hepatic stellate cells activation, and induction of apoptosis.

Authors+Show Affiliations

a Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, Adakahlia 35516, Egypt.b Department of Pathology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

27010252

Citation

Abu-Elsaad, Nashwa M., and Wagdi Fawzi Elkashef. "Modified Citrus Pectin Stops Progression of Liver Fibrosis By Inhibiting Galectin-3 and Inducing Apoptosis of Stellate Cells." Canadian Journal of Physiology and Pharmacology, vol. 94, no. 5, 2016, pp. 554-62.
Abu-Elsaad NM, Elkashef WF. Modified citrus pectin stops progression of liver fibrosis by inhibiting galectin-3 and inducing apoptosis of stellate cells. Can J Physiol Pharmacol. 2016;94(5):554-62.
Abu-Elsaad, N. M., & Elkashef, W. F. (2016). Modified citrus pectin stops progression of liver fibrosis by inhibiting galectin-3 and inducing apoptosis of stellate cells. Canadian Journal of Physiology and Pharmacology, 94(5), 554-62. https://doi.org/10.1139/cjpp-2015-0284
Abu-Elsaad NM, Elkashef WF. Modified Citrus Pectin Stops Progression of Liver Fibrosis By Inhibiting Galectin-3 and Inducing Apoptosis of Stellate Cells. Can J Physiol Pharmacol. 2016;94(5):554-62. PubMed PMID: 27010252.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modified citrus pectin stops progression of liver fibrosis by inhibiting galectin-3 and inducing apoptosis of stellate cells. AU - Abu-Elsaad,Nashwa M, AU - Elkashef,Wagdi Fawzi, Y1 - 2015/12/16/ PY - 2016/3/25/entrez PY - 2016/3/25/pubmed PY - 2017/2/14/medline KW - antioxidant KW - antioxydants KW - apoptose KW - apoptosis KW - citrus pectin KW - fibrose KW - fibrosis KW - foie KW - galectin-3 KW - galectine-3 KW - liver KW - pectine d’agrumes SP - 554 EP - 62 JF - Canadian journal of physiology and pharmacology JO - Can. J. Physiol. Pharmacol. VL - 94 IS - 5 N2 - Modified citrus pectin (MCP) is a pH modified form of the dietary soluble citrus peel fiber known as pectin. The current study aims at testing its effect on liver fibrosis progression. Rats were injected with CCl4 (1 mL/kg, 40% v/v, i.p., twice a week for 8 weeks). Concurrently, MCP (400 or 1200 mg/kg) was administered daily in drinking water from the first week in groups I and II (prophylactic model) and in the beginning of week 5 in groups III and IV (therapeutic model). Liver function biomarkers (ATL, AST, and ALP), fibrosis markers (laminin and hyaluronic acid), and antioxidant biomarkers (reduced glutathione (GSH) and superoxide dismutase (SOD)) were measured. Stained liver sections were scored for fibrosis and necroinflammation. Additionally, expression of galectin-3 (Gal-3), α-smooth muscle actin (SMA), tissue inhibitor metalloproteinase (TIMP)-1, collagen (Col)1A1, caspase (Cas)-3, and apoptosis related factor (FAS) were assigned. Modified pectin late administration significantly (p < 0.05) decreased malondialdehyde (MDA), TIMP-1, Col1A1, α-SMA, and Gal-3 levels and increased levels of FAS, Cas-3, GSH, and SOD. It also decreased percentage of fibrosis and necroinflammation significantly (p < 0.05). It can be concluded that MCP can attenuate liver fibrosis through an antioxidant effect, inhibition of Gal-3 mediated hepatic stellate cells activation, and induction of apoptosis. SN - 1205-7541 UR - https://www.unboundmedicine.com/medline/citation/27010252/Modified_citrus_pectin_stops_progression_of_liver_fibrosis_by_inhibiting_galectin_3_and_inducing_apoptosis_of_stellate_cells_ L2 - http://www.nrcresearchpress.com/doi/full/10.1139/cjpp-2015-0284?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -