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Regular exercise alleviates renovascular hypertension-induced cardiac/endothelial dysfunction and oxidative injury in rats.
J Physiol Pharmacol. 2016 Feb; 67(1):45-55.JP

Abstract

The importance of physical activity in the management of renovascular diseases is well-known, but lacks evidence of underlying mechanisms. The purpose of the study was to elucidate the protective/therapeutic effects of regular exercise on experimental renovascular hypertension (RVH)-induced oxidative stress and cardiac dysfunction. Wistar albino rats underwent a RVH surgery (2K1C, Goldblatt). Three weeks later half of the rats started swimming exercise for 9 weeks (n = 15), while the sedentary RVH group (n = 15) had no exercise during that period. Sham-operated control rats (n = 10), had the similar surgical procedures but the left renal artery was left unclipped. Body weights were monitored, and blood pressures were measured weekly using tail-cuff. Echocardiographic evaluation was performed on the 3(rd) week and on the 12(th) week of the experiment before the rats were decapitated. Heart and thoracic aorta were removed and serum was collected, while aortic samples were put in a 10% formaldehyde solution for immunochemistry. Cardiac tissue samples obtained from each animal were used for the determination of tissue myeloperoxidase (MPO) and catalase (CAT) activities, malondialdehyde (MDA), and glutathione (GSH) levels. In the sedentary RVH group, aortic contractile response (contraction/relaxation in isolated organ bath), left ventricular diastolic and systolic dimensions, and immunohistochemical staining of aortic inducible nitric oxide synthase (iNOS) were increased, while ejection fraction and aortic endothelial nitric oxide synthase (eNOS) staining were decreased. RVH in the sedentary rats resulted in increased pro-inflammatory cytokines (TNF-α, IL-2, IL-6), lipid peroxidation (malondialdehyde) and neutrophil infiltration (myeloperoxidase activity) along with reductions in antioxidant glutathione and catalase levels in the cardiac tissue. Exercise after RVH increased the immunohistochemical staining of aortic eNOS, decreased iNOS staining and reversed the alterations in echocardiographic and oxidative parameters. Regular exercise commenced after RVH surgery alleviated renovascular hypertension-induced oxidative injury, by modulating oxidant-antioxidant balance via the involvement of the endothelial NO system.

Authors+Show Affiliations

Department of Physiology, School of Medicine, Marmara University, Istanbul, Turkey.Faculty of Pharmacy, Department of Pharmacology, Faculty of Pharmacy, Marmara University, Istanbul, Turkey.Department of Biochemistry, Faculty of Dentistry, School of Medicine, Marmara University, Istanbul, Turkey.Department of Nephrology, School of Medicine, Marmara University, Istanbul, Turkey.Department of Cardiology, Gulhane Military Academy, Ankara, Turkey.Department of Histology and Embryology, Istanbul Bilim University, Istanbul, Turkey.Department of Physiology, School of Medicine, Marmara University, Istanbul, Turkey. byegen@marmara.edu.tr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27010894

Citation

Kumral, Z N O., et al. "Regular Exercise Alleviates Renovascular Hypertension-induced Cardiac/endothelial Dysfunction and Oxidative Injury in Rats." Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, vol. 67, no. 1, 2016, pp. 45-55.
Kumral ZN, Sener G, Ozgur S, et al. Regular exercise alleviates renovascular hypertension-induced cardiac/endothelial dysfunction and oxidative injury in rats. J Physiol Pharmacol. 2016;67(1):45-55.
Kumral, Z. N., Sener, G., Ozgur, S., Koc, M., Suleymanoglu, S., Hurdag, C., & Yegen, B. C. (2016). Regular exercise alleviates renovascular hypertension-induced cardiac/endothelial dysfunction and oxidative injury in rats. Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, 67(1), 45-55.
Kumral ZN, et al. Regular Exercise Alleviates Renovascular Hypertension-induced Cardiac/endothelial Dysfunction and Oxidative Injury in Rats. J Physiol Pharmacol. 2016;67(1):45-55. PubMed PMID: 27010894.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regular exercise alleviates renovascular hypertension-induced cardiac/endothelial dysfunction and oxidative injury in rats. AU - Kumral,Z N O, AU - Sener,G, AU - Ozgur,S, AU - Koc,M, AU - Suleymanoglu,S, AU - Hurdag,C, AU - Yegen,B C, PY - 2015/07/15/received PY - 2016/01/19/accepted PY - 2016/3/25/entrez PY - 2016/3/25/pubmed PY - 2016/12/15/medline SP - 45 EP - 55 JF - Journal of physiology and pharmacology : an official journal of the Polish Physiological Society JO - J Physiol Pharmacol VL - 67 IS - 1 N2 - The importance of physical activity in the management of renovascular diseases is well-known, but lacks evidence of underlying mechanisms. The purpose of the study was to elucidate the protective/therapeutic effects of regular exercise on experimental renovascular hypertension (RVH)-induced oxidative stress and cardiac dysfunction. Wistar albino rats underwent a RVH surgery (2K1C, Goldblatt). Three weeks later half of the rats started swimming exercise for 9 weeks (n = 15), while the sedentary RVH group (n = 15) had no exercise during that period. Sham-operated control rats (n = 10), had the similar surgical procedures but the left renal artery was left unclipped. Body weights were monitored, and blood pressures were measured weekly using tail-cuff. Echocardiographic evaluation was performed on the 3(rd) week and on the 12(th) week of the experiment before the rats were decapitated. Heart and thoracic aorta were removed and serum was collected, while aortic samples were put in a 10% formaldehyde solution for immunochemistry. Cardiac tissue samples obtained from each animal were used for the determination of tissue myeloperoxidase (MPO) and catalase (CAT) activities, malondialdehyde (MDA), and glutathione (GSH) levels. In the sedentary RVH group, aortic contractile response (contraction/relaxation in isolated organ bath), left ventricular diastolic and systolic dimensions, and immunohistochemical staining of aortic inducible nitric oxide synthase (iNOS) were increased, while ejection fraction and aortic endothelial nitric oxide synthase (eNOS) staining were decreased. RVH in the sedentary rats resulted in increased pro-inflammatory cytokines (TNF-α, IL-2, IL-6), lipid peroxidation (malondialdehyde) and neutrophil infiltration (myeloperoxidase activity) along with reductions in antioxidant glutathione and catalase levels in the cardiac tissue. Exercise after RVH increased the immunohistochemical staining of aortic eNOS, decreased iNOS staining and reversed the alterations in echocardiographic and oxidative parameters. Regular exercise commenced after RVH surgery alleviated renovascular hypertension-induced oxidative injury, by modulating oxidant-antioxidant balance via the involvement of the endothelial NO system. SN - 1899-1505 UR - https://www.unboundmedicine.com/medline/citation/27010894/Regular_exercise_alleviates_renovascular_hypertension_induced_cardiac/endothelial_dysfunction_and_oxidative_injury_in_rats_ L2 - http://www.jpp.krakow.pl/journal/archive/02_16/pdf/45_02_16_article.pdf DB - PRIME DP - Unbound Medicine ER -