Fluorescent aptasensor for chloramphenicol detection using DIL-encapsulated liposome as nanotracer.
Biosens Bioelectron. 2016 Jul 15; 81:454-459.BB

Abstract

A novel fluorescence aptasensor was successfully developed to respond to chloramphenicol (CAP) in food based on magnetic aptamer-liposome vesicle probe. In order to fabricate it, aptamer labeled on functionalized magnetic beads (MB) was firstly employed as capture adsorbent (MB-Apt), then SSB (single-stranded DNA binding protein) and DIL (1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanineperchlorate) coimmobilized liposomes (SSB/DIL-Lip) was employed as vesicle signal tracer. The composite vesicle probe is formed between SSB/DIL-Lip and MB-Apt based on SSB's specific recognition towards aptamer on vesicle signal tracer. Upon the vesicle probe solution reacted with CAP, the aptamer on the magnetic beads preferentially bounded with CAP, and then released SSB/DIL-Lip vesicle signal tracer in the supernatant after magnetic separation. The released tracer can emit fluorescence which was correspondence with the concentration of the analyte. At the optimum conditions, the aptasensor exhibited a good linear response for CAP detection in the range of 0.003-10nM with a detection limit of 1pM. Importantly, the methodology was further validated for analyzing CAP in fish samples with consistent results obtained by ELISA kit, thus providing a promising approach for quantitative monitoring of CAP and significant anti-interference ability in food safety.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Miao YB

State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.

Ren HX

Key Laboratory of Asymmetric Synthesis and Chirotechnology of Sichuan Province, Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, PR China; University of Chinese Academy of Sciences, Beijing 10049, PR China.

Gan N

State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China. Electronic address: ganning@nbu.edu.cn.

Cao Y

Li T

State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.

Chen Y

Faculty of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210000, PR China.

MeSH

AnimalsAnti-Bacterial AgentsAptamers, NucleotideBiosensing TechniquesChloramphenicolFishesFluorescent DyesFood AnalysisImmobilized ProteinsLimit of DetectionLiposomesMagnetsPerchloratesSeafoodSpectrometry, Fluorescence

Pub Type(s)

Evaluation Study

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27015148

Citation

Miao, Yang-Bao, et al. "Fluorescent Aptasensor for Chloramphenicol Detection Using DIL-encapsulated Liposome as Nanotracer." Biosensors & Bioelectronics, vol. 81, 2016, pp. 454-459.

Miao YB, Ren HX, Gan N, et al. Fluorescent aptasensor for chloramphenicol detection using DIL-encapsulated liposome as nanotracer. Biosens Bioelectron. 2016;81:454-459.

Miao, Y. B., Ren, H. X., Gan, N., Cao, Y., Li, T., & Chen, Y. (2016). Fluorescent aptasensor for chloramphenicol detection using DIL-encapsulated liposome as nanotracer. Biosensors & Bioelectronics, 81, 454-459. https://doi.org/10.1016/j.bios.2016.03.034

Miao YB, et al. Fluorescent Aptasensor for Chloramphenicol Detection Using DIL-encapsulated Liposome as Nanotracer. Biosens Bioelectron. 2016 Jul 15;81:454-459. PubMed PMID: 27015148.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Fluorescent aptasensor for chloramphenicol detection using DIL-encapsulated liposome as nanotracer. AU - Miao,Yang-Bao, AU - Ren,Hong-Xia, AU - Gan,Ning, AU - Cao,Yuting, AU - Li,Tianhua, AU - Chen,Yijin, Y1 - 2016/03/16/ PY - 2016/01/19/received PY - 2016/03/12/revised PY - 2016/03/15/accepted PY - 2016/3/26/entrez PY - 2016/3/26/pubmed PY - 2017/1/11/medline KW - Chloramphenicol KW - DIL KW - Fluorescence aptasensor KW - Magnetic liposome vesicle signal tracer KW - Single-stranded DNA binding protein SP - 454 EP - 459 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 81 N2 - A novel fluorescence aptasensor was successfully developed to respond to chloramphenicol (CAP) in food based on magnetic aptamer-liposome vesicle probe. In order to fabricate it, aptamer labeled on functionalized magnetic beads (MB) was firstly employed as capture adsorbent (MB-Apt), then SSB (single-stranded DNA binding protein) and DIL (1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanineperchlorate) coimmobilized liposomes (SSB/DIL-Lip) was employed as vesicle signal tracer. The composite vesicle probe is formed between SSB/DIL-Lip and MB-Apt based on SSB's specific recognition towards aptamer on vesicle signal tracer. Upon the vesicle probe solution reacted with CAP, the aptamer on the magnetic beads preferentially bounded with CAP, and then released SSB/DIL-Lip vesicle signal tracer in the supernatant after magnetic separation. The released tracer can emit fluorescence which was correspondence with the concentration of the analyte. At the optimum conditions, the aptasensor exhibited a good linear response for CAP detection in the range of 0.003-10nM with a detection limit of 1pM. Importantly, the methodology was further validated for analyzing CAP in fish samples with consistent results obtained by ELISA kit, thus providing a promising approach for quantitative monitoring of CAP and significant anti-interference ability in food safety. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/27015148/Fluorescent_aptasensor_for_chloramphenicol_detection_using_DIL_encapsulated_liposome_as_nanotracer_ DB - PRIME DP - Unbound Medicine ER -

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Fluorescent aptasensor for chloramphenicol detection using DIL-encapsulated liposome as nanotracer.Biosens Bioelectron. 2016 Jul 15; 81:454-459.BB