Tags

Type your tag names separated by a space and hit enter

Exposure to benzene metabolites causes oxidative damage in Saccharomyces cerevisiae.
Antonie Van Leeuwenhoek. 2016 Jun; 109(6):841-54.AV

Abstract

Hydroquinone (HQ) and benzoquinone (BQ) are known benzene metabolites that form reactive intermediates such as reactive oxygen species (ROS). This study attempts to understand the effect of benzene metabolites (HQ and BQ) on the antioxidant status, cell morphology, ROS levels and lipid alterations in the yeast Saccharomyces cerevisiae. There was a reduction in the growth pattern of wild-type cells exposed to HQ/BQ. Exposure of yeast cells to benzene metabolites increased the activity of the anti-oxidant enzymes catalase, superoxide dismutase and glutathione peroxidase but lead to a decrease in ascorbic acid and reduced glutathione. Increased triglyceride level and decreased phospholipid levels were observed with exposure to HQ and BQ. These results suggest that the enzymatic antioxidants were increased and are involved in the protection against macromolecular damage during oxidative stress; presumptively, these enzymes are essential for scavenging the pro-oxidant effects of benzene metabolites.

Authors+Show Affiliations

Biomembrane Lab, Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamilnadu, 620024, India.Biomembrane Lab, Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamilnadu, 620024, India. vasanthibch@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27016252

Citation

Raj, Abhishek, and Vasanthi Nachiappan. "Exposure to Benzene Metabolites Causes Oxidative Damage in Saccharomyces Cerevisiae." Antonie Van Leeuwenhoek, vol. 109, no. 6, 2016, pp. 841-54.
Raj A, Nachiappan V. Exposure to benzene metabolites causes oxidative damage in Saccharomyces cerevisiae. Antonie Van Leeuwenhoek. 2016;109(6):841-54.
Raj, A., & Nachiappan, V. (2016). Exposure to benzene metabolites causes oxidative damage in Saccharomyces cerevisiae. Antonie Van Leeuwenhoek, 109(6), 841-54. https://doi.org/10.1007/s10482-016-0684-7
Raj A, Nachiappan V. Exposure to Benzene Metabolites Causes Oxidative Damage in Saccharomyces Cerevisiae. Antonie Van Leeuwenhoek. 2016;109(6):841-54. PubMed PMID: 27016252.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exposure to benzene metabolites causes oxidative damage in Saccharomyces cerevisiae. AU - Raj,Abhishek, AU - Nachiappan,Vasanthi, Y1 - 2016/03/25/ PY - 2016/01/03/received PY - 2016/03/17/accepted PY - 2016/3/27/entrez PY - 2016/3/27/pubmed PY - 2017/3/4/medline KW - Antioxidants KW - BQ KW - HQ KW - Lipid KW - ROS KW - Yeast SP - 841 EP - 54 JF - Antonie van Leeuwenhoek JO - Antonie Van Leeuwenhoek VL - 109 IS - 6 N2 - Hydroquinone (HQ) and benzoquinone (BQ) are known benzene metabolites that form reactive intermediates such as reactive oxygen species (ROS). This study attempts to understand the effect of benzene metabolites (HQ and BQ) on the antioxidant status, cell morphology, ROS levels and lipid alterations in the yeast Saccharomyces cerevisiae. There was a reduction in the growth pattern of wild-type cells exposed to HQ/BQ. Exposure of yeast cells to benzene metabolites increased the activity of the anti-oxidant enzymes catalase, superoxide dismutase and glutathione peroxidase but lead to a decrease in ascorbic acid and reduced glutathione. Increased triglyceride level and decreased phospholipid levels were observed with exposure to HQ and BQ. These results suggest that the enzymatic antioxidants were increased and are involved in the protection against macromolecular damage during oxidative stress; presumptively, these enzymes are essential for scavenging the pro-oxidant effects of benzene metabolites. SN - 1572-9699 UR - https://www.unboundmedicine.com/medline/citation/27016252/Exposure_to_benzene_metabolites_causes_oxidative_damage_in_Saccharomyces_cerevisiae_ L2 - https://doi.org/10.1007/s10482-016-0684-7 DB - PRIME DP - Unbound Medicine ER -