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Serologic and molecular evidence of Brucella ovis infection in ovine and caprine flocks in the State of Minas Gerais, Brazil.
BMC Res Notes. 2016 Mar 26; 9:190.BR

Abstract

BACKGROUND

Brucella ovis infection is one of the leading causes of sub fertility and infertility in ovine, been characterized mainly by epididymitis, orchitis and testicular atrophy in rams. This study aimed to determine the frequency of B. ovis positivity in rams and goats flocks in the State of Minas Gerais, Brazil, by agarose gel immunodiffusion (AGID), ELISA, Rose Bengal, PCR and bacteriological isolation as diagnostic tools.

FINDINGS

Serum and urine samples were collected from properties with sheep or goat flocks, or from properties with mixed flock. Out of 50 sheep flocks, 6% (3/50) were seropositive by AGID while 4% (2/50) were positive by urine PCR for B. ovis. Out of five goat farms, 20% (1/5) were seropositive for B. ovis by AGID. Mixed flock farms had 11.1% (2/18) of positivity by AGID. By ELISA, 19.5% (8/41) of sheep properties and 61.1% (11/18) of the properties with mixed flocks were positive for B. ovis. No samples were positive in the test of Rose Bengal, ruling out exposure to smooth LPS Brucella species (particularly Brucella melitensis) and indicating that the positive in the ELISA was associated with Brucella spp. LPS rough (presumably B. ovis). No urine sample from sheep or goat was positive by bacteriological isolation.

CONCLUSIONS

Our results demonstrate serologic or molecular evidence of B. ovis infection in several rams and billy goats from meso-regions of the State of Minas Gerais, Brazil. Also, this study report the indirect ELISA as an important tool for the diagnosis of B. ovis infection, as indirect ELISA in this study demonstrated to be the most sensitive diagnostic method adopted.

Authors+Show Affiliations

Departamento de Patologia, Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, CEP 31.270-901, Belo Horizonte, MG, Brazil.Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária da Universidade Federal Minas Gerais, CEP 31.270-901, Belo Horizonte, MG, Brazil.Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária da Universidade Federal Minas Gerais, CEP 31.270-901, Belo Horizonte, MG, Brazil.Instituto de Ciências Agrárias da Universidade Federal de Minas Gerais, CEP 39.404-006, Montes Claros, MG, Brazil.Instituto de Ciências Agrárias da Universidade Federal de Minas Gerais, CEP 39.404-006, Montes Claros, MG, Brazil.Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária da Universidade Federal Minas Gerais, CEP 31.270-901, Belo Horizonte, MG, Brazil.Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária da Universidade Federal Minas Gerais, CEP 31.270-901, Belo Horizonte, MG, Brazil.Instituto de Ciências Agrárias da Universidade Federal de Minas Gerais, CEP 39.404-006, Montes Claros, MG, Brazil.Departamento de Medicina Veterinária Preventiva, Escola de Veterinária da Universidade Federal Minas Gerais, CEP 31.270-901, Belo Horizonte, MG, Brazil.Departamento de Medicina Veterinária Preventiva, Escola de Veterinária da Universidade Federal Minas Gerais, CEP 31.270-901, Belo Horizonte, MG, Brazil.Departamento de Patologia, Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, CEP 31.270-901, Belo Horizonte, MG, Brazil.Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária da Universidade Federal Minas Gerais, CEP 31.270-901, Belo Horizonte, MG, Brazil. rsantos@vet.ufmg.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27017445

Citation

Costa, Luciana Fachini, et al. "Serologic and Molecular Evidence of Brucella Ovis Infection in Ovine and Caprine Flocks in the State of Minas Gerais, Brazil." BMC Research Notes, vol. 9, 2016, p. 190.
Costa LF, Pessoa MS, Guimarães LB, et al. Serologic and molecular evidence of Brucella ovis infection in ovine and caprine flocks in the State of Minas Gerais, Brazil. BMC Res Notes. 2016;9:190.
Costa, L. F., Pessoa, M. S., Guimarães, L. B., Faria, A. K., Morão, R. P., Mol, J. P., Garcia, L. N., Almeida, A. C., Gouveia, A. M., Silva, M. X., Paixão, T. A., & Santos, R. L. (2016). Serologic and molecular evidence of Brucella ovis infection in ovine and caprine flocks in the State of Minas Gerais, Brazil. BMC Research Notes, 9, 190. https://doi.org/10.1186/s13104-016-1998-2
Costa LF, et al. Serologic and Molecular Evidence of Brucella Ovis Infection in Ovine and Caprine Flocks in the State of Minas Gerais, Brazil. BMC Res Notes. 2016 Mar 26;9:190. PubMed PMID: 27017445.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serologic and molecular evidence of Brucella ovis infection in ovine and caprine flocks in the State of Minas Gerais, Brazil. AU - Costa,Luciana Fachini, AU - Pessoa,Moisés Sena, AU - Guimarães,Laís Bitencourt, AU - Faria,Anne Karoene Silva, AU - Morão,Rodrigo Pereira, AU - Mol,Juliana Pinto da Silva, AU - Garcia,Luize Néli Nunes, AU - Almeida,Anna Christina, AU - Gouveia,Aurora Maria Guimarães, AU - Silva,Marcos Xavier, AU - Paixão,Tatiane Alves, AU - Santos,Renato Lima, Y1 - 2016/03/26/ PY - 2015/04/05/received PY - 2016/03/18/accepted PY - 2016/3/28/entrez PY - 2016/3/29/pubmed PY - 2016/12/15/medline KW - Brucella ovis KW - Brucellosis KW - Goats KW - Rams SP - 190 EP - 190 JF - BMC research notes JO - BMC Res Notes VL - 9 N2 - BACKGROUND: Brucella ovis infection is one of the leading causes of sub fertility and infertility in ovine, been characterized mainly by epididymitis, orchitis and testicular atrophy in rams. This study aimed to determine the frequency of B. ovis positivity in rams and goats flocks in the State of Minas Gerais, Brazil, by agarose gel immunodiffusion (AGID), ELISA, Rose Bengal, PCR and bacteriological isolation as diagnostic tools. FINDINGS: Serum and urine samples were collected from properties with sheep or goat flocks, or from properties with mixed flock. Out of 50 sheep flocks, 6% (3/50) were seropositive by AGID while 4% (2/50) were positive by urine PCR for B. ovis. Out of five goat farms, 20% (1/5) were seropositive for B. ovis by AGID. Mixed flock farms had 11.1% (2/18) of positivity by AGID. By ELISA, 19.5% (8/41) of sheep properties and 61.1% (11/18) of the properties with mixed flocks were positive for B. ovis. No samples were positive in the test of Rose Bengal, ruling out exposure to smooth LPS Brucella species (particularly Brucella melitensis) and indicating that the positive in the ELISA was associated with Brucella spp. LPS rough (presumably B. ovis). No urine sample from sheep or goat was positive by bacteriological isolation. CONCLUSIONS: Our results demonstrate serologic or molecular evidence of B. ovis infection in several rams and billy goats from meso-regions of the State of Minas Gerais, Brazil. Also, this study report the indirect ELISA as an important tool for the diagnosis of B. ovis infection, as indirect ELISA in this study demonstrated to be the most sensitive diagnostic method adopted. SN - 1756-0500 UR - https://www.unboundmedicine.com/medline/citation/27017445/Serologic_and_molecular_evidence_of_Brucella_ovis_infection_in_ovine_and_caprine_flocks_in_the_State_of_Minas_Gerais_Brazil_ L2 - https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-016-1998-2 DB - PRIME DP - Unbound Medicine ER -