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Characterization of partial NS5B region among hepatitis C virus genotype 6 subtypes isolated from Thai blood donors.
J Med Virol. 2016 10; 88(10):1785-90.JM

Abstract

The hepatitis C virus (HCV) is an important cause of liver dysfunction which continues to spread in Thailand, particularly as genotype 6. The NS5B gene fragment is particularly variable and thus provides a valuable tracker for its spread. Therefore, the purpose of this study was to characterize the HCV genotype 6 based on partial NS5B region using Thai blood donor samples. Twenty-nine samples were genotyped as HCV 6 by nested PCR, nucleotide sequencing and amino acid sequence analysis. Amplified products were identified as HCV genotypes 6f, 6c, 6n, and 6i. There were amino acid variations of 4-18 residues in subtypes 6f, 6c, and 6n whereas subtype 6i was conserved when compared with their referent strains. In subtypes 6f, 6c, 6n, and 6i, the amino acid mutations at positions 244, 309, and 310 which are associated with HCV resistance were present. In summary, the sequences and phylogenetic analysis of NS5B of HCV used in our study yielded the genotypes 6f, 6c, 6n, and 6i. This finding indicates diversity of amino acids in NS5B of HCV. Characterizing the partial NS5B region among hepatitis C virus genotype 6 subtypes may predict efficacious anti-HCV therapy. J. Med. Virol. 88:1785-1790, 2016. © 2016 Wiley Periodicals, Inc.

Authors+Show Affiliations

Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand.Faculty of Medical Science, Department of Microbiology and Parasitology, Naresuan University, Phitsanulok, Thailand.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27018495

Citation

Chaiwong, Suriya, and Anchalee Sistayanarain. "Characterization of Partial NS5B Region Among Hepatitis C Virus Genotype 6 Subtypes Isolated From Thai Blood Donors." Journal of Medical Virology, vol. 88, no. 10, 2016, pp. 1785-90.
Chaiwong S, Sistayanarain A. Characterization of partial NS5B region among hepatitis C virus genotype 6 subtypes isolated from Thai blood donors. J Med Virol. 2016;88(10):1785-90.
Chaiwong, S., & Sistayanarain, A. (2016). Characterization of partial NS5B region among hepatitis C virus genotype 6 subtypes isolated from Thai blood donors. Journal of Medical Virology, 88(10), 1785-90. https://doi.org/10.1002/jmv.24536
Chaiwong S, Sistayanarain A. Characterization of Partial NS5B Region Among Hepatitis C Virus Genotype 6 Subtypes Isolated From Thai Blood Donors. J Med Virol. 2016;88(10):1785-90. PubMed PMID: 27018495.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of partial NS5B region among hepatitis C virus genotype 6 subtypes isolated from Thai blood donors. AU - Chaiwong,Suriya, AU - Sistayanarain,Anchalee, Y1 - 2016/04/05/ PY - 2016/03/24/accepted PY - 2016/3/29/entrez PY - 2016/3/29/pubmed PY - 2017/8/2/medline KW - Thai blood donors KW - amino acid analysis KW - genotype 6 KW - hepatitis C virus (HCV) KW - nonstructural 5B gene (NS5B) SP - 1785 EP - 90 JF - Journal of medical virology JO - J Med Virol VL - 88 IS - 10 N2 - The hepatitis C virus (HCV) is an important cause of liver dysfunction which continues to spread in Thailand, particularly as genotype 6. The NS5B gene fragment is particularly variable and thus provides a valuable tracker for its spread. Therefore, the purpose of this study was to characterize the HCV genotype 6 based on partial NS5B region using Thai blood donor samples. Twenty-nine samples were genotyped as HCV 6 by nested PCR, nucleotide sequencing and amino acid sequence analysis. Amplified products were identified as HCV genotypes 6f, 6c, 6n, and 6i. There were amino acid variations of 4-18 residues in subtypes 6f, 6c, and 6n whereas subtype 6i was conserved when compared with their referent strains. In subtypes 6f, 6c, 6n, and 6i, the amino acid mutations at positions 244, 309, and 310 which are associated with HCV resistance were present. In summary, the sequences and phylogenetic analysis of NS5B of HCV used in our study yielded the genotypes 6f, 6c, 6n, and 6i. This finding indicates diversity of amino acids in NS5B of HCV. Characterizing the partial NS5B region among hepatitis C virus genotype 6 subtypes may predict efficacious anti-HCV therapy. J. Med. Virol. 88:1785-1790, 2016. © 2016 Wiley Periodicals, Inc. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/27018495/Characterization_of_partial_NS5B_region_among_hepatitis_C_virus_genotype_6_subtypes_isolated_from_Thai_blood_donors_ L2 - https://doi.org/10.1002/jmv.24536 DB - PRIME DP - Unbound Medicine ER -