GENETIC POPULATION STRUCTURE AND MATING SYSTEM IN CHONDRUS CRISPUS (RHODOPHYTA)1.
J Phycol. 2011 Jun; 47(3):440-450.JP

Abstract

Chondrus crispus Stackh. has been intensely studied, yet no study to date has elucidated its population structure or mating system despite many populations in which there was a haploid bias and lack of male gametophytes. Therefore, 12 nuclear microsatellite loci were identified in this red alga. Microsatellite markers were developed and tested against a panel of specimens collected from two shore levels at two sites in Brittany, France: Pointe de Primel and Pointe de la Jument, Concarneau. Single locus genetic determinism was verified at eight polymorphic loci, as only one band was observed for haploid genotypes, whereas one or two bands were observed for diploids. These markers enabled the detection of unique genotypes within sampled populations, indicating that very few fronds shared the same multilocus genotype. This finding suggests that asexual reproduction was not the prevailing mode of reproduction. In addition, we explored the hierarchical population structure showing that gene flow is restricted at small spatial scales (<50 m) between upper and lower Chondrus-range populations within a shore. Sexual reproduction predominated in the populations of C. crispus studied, but probably due to fine-scale spatial substructuring, inbreeding was also significant. In conclusion, this study reveals that fine-scale genetic variation is of major importance in C. crispus, suggesting that differences between microhabitats should be essential in understanding evolutionary processes in this species.

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Authors+Show Affiliations

Krueger-Hadfield SA

UPMC Univ Paris 06, UMR7144, Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, Place Georges Teissier, 29682 Roscoff, France CNRS, UMR7144, Station Biologique de Roscoff, France Department of Biology, California State University Northridge, 18111 Nordhoff St., Northridge, California 91330-8303, USAUPMC Univ Paris 06, UMR7139, Végétaux Marins et Biomolécules, Station Biologique de Roscoff, Place Georges Teissier, 29682 Roscoff, FranceUPMC Univ Paris 06, UMR7144, Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, Place Georges Teissier, 29682 Roscoff, France CNRS, UMR7144, Station Biologique de Roscoff, France.

Collén J

Daguin-Thiébaut C

Valero M

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27021973

Citation

Krueger-Hadfield, Stacy A., et al. "GENETIC POPULATION STRUCTURE and MATING SYSTEM in CHONDRUS CRISPUS (RHODOPHYTA)1." Journal of Phycology, vol. 47, no. 3, 2011, pp. 440-450.

Krueger-Hadfield SA, Collén J, Daguin-Thiébaut C, et al. GENETIC POPULATION STRUCTURE AND MATING SYSTEM IN CHONDRUS CRISPUS (RHODOPHYTA)1. J Phycol. 2011;47(3):440-450.

Krueger-Hadfield, S. A., Collén, J., Daguin-Thiébaut, C., & Valero, M. (2011). GENETIC POPULATION STRUCTURE AND MATING SYSTEM IN CHONDRUS CRISPUS (RHODOPHYTA)1. Journal of Phycology, 47(3), 440-450. https://doi.org/10.1111/j.1529-8817.2011.00995.x

Krueger-Hadfield SA, et al. GENETIC POPULATION STRUCTURE and MATING SYSTEM in CHONDRUS CRISPUS (RHODOPHYTA)1. J Phycol. 2011;47(3):440-450. PubMed PMID: 27021973.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - GENETIC POPULATION STRUCTURE AND MATING SYSTEM IN CHONDRUS CRISPUS (RHODOPHYTA)1. AU - Krueger-Hadfield,Stacy A, AU - Collén,Jonas, AU - Daguin-Thiébaut,Claire, AU - Valero,Myriam, Y1 - 2011/05/04/ PY - 2016/3/30/entrez PY - 2011/6/1/pubmed PY - 2011/6/1/medline KW - dispersal KW - gametophyte KW - gene flow KW - haploid-diploid KW - inbreeding KW - isomorphic life cycle KW - microsatellites KW - tetrasporophyte SP - 440 EP - 450 JF - Journal of phycology JO - J Phycol VL - 47 IS - 3 N2 - Chondrus crispus Stackh. has been intensely studied, yet no study to date has elucidated its population structure or mating system despite many populations in which there was a haploid bias and lack of male gametophytes. Therefore, 12 nuclear microsatellite loci were identified in this red alga. Microsatellite markers were developed and tested against a panel of specimens collected from two shore levels at two sites in Brittany, France: Pointe de Primel and Pointe de la Jument, Concarneau. Single locus genetic determinism was verified at eight polymorphic loci, as only one band was observed for haploid genotypes, whereas one or two bands were observed for diploids. These markers enabled the detection of unique genotypes within sampled populations, indicating that very few fronds shared the same multilocus genotype. This finding suggests that asexual reproduction was not the prevailing mode of reproduction. In addition, we explored the hierarchical population structure showing that gene flow is restricted at small spatial scales (<50 m) between upper and lower Chondrus-range populations within a shore. Sexual reproduction predominated in the populations of C. crispus studied, but probably due to fine-scale spatial substructuring, inbreeding was also significant. In conclusion, this study reveals that fine-scale genetic variation is of major importance in C. crispus, suggesting that differences between microhabitats should be essential in understanding evolutionary processes in this species. SN - 1529-8817 UR - https://www.unboundmedicine.com/medline/citation/27021973/GENETIC_POPULATION_STRUCTURE_AND_MATING_SYSTEM_IN_CHONDRUS_CRISPUS__RHODOPHYTA_1_ DB - PRIME DP - Unbound Medicine ER -

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GENETIC POPULATION STRUCTURE AND MATING SYSTEM IN CHONDRUS CRISPUS (RHODOPHYTA)1.J Phycol. 2011 Jun; 47(3):440-450.JP