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Neutralization of the Principal Toxins from the Venoms of Thai Naja kaouthia and Malaysian Hydrophis schistosus: Insights into Toxin-Specific Neutralization by Two Different Antivenoms.
Toxins (Basel). 2016 Mar 26; 8(4):86.T

Abstract

Antivenom neutralization against cobra venoms is generally low in potency, presumably due to poor toxin-specific immunoreactivity. This study aimed to investigate the effectiveness of two elapid antivenoms to neutralize the principal toxins purified from the venoms of the Thai monocled cobra (Naja kaouthia, Nk-T) and the Malaysian beaked sea snake (Hydrophis schistosus, Hs-M). In mice, N. kaouthia Monovalent Antivenom (NKMAV) neutralization against Nk-T long neurotoxin (LNTX) and cytotoxin was moderate (potency of 2.89-6.44 mg toxin/g antivenom protein) but poor against the short neurotoxin (SNTX) (1.33 mg/g). Its cross-neutralization against Hs-M LNTX of Hs-M is compatible (0.18 mg/g) but much weaker against Hs-M SNTX (0.22 mg/g). Using CSL (Seqirus Limited) Sea Snake Antivenom (SSAV), we observed consistently weak neutralization of antivenom against SNTX of both species, suggesting that this is the limiting factor on the potency of antivenom neutralization against venoms containing SNTX. Nevertheless, SSAV outperformed NKMAV in neutralizing SNTXs of both species (0.61-2.49 mg/g). The superior efficacy of SSAV against SNTX is probably partly attributable to the high abundance of SNTX in sea snake venom used as immunogen in SSAV production. The findings indicate that improving the potency of cobra antivenom may be possible with a proper immunogen formulation that seeks to overcome the limitation on SNTX immunoreactivity.

Authors+Show Affiliations

Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. kytan_kae@yahoo.com.Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. tanchoohock@gmail.com.Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. fungshinyee@gmail.com.Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. tanngethong@yahoo.com.sg.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27023606

Citation

Tan, Kae Yi, et al. "Neutralization of the Principal Toxins From the Venoms of Thai Naja Kaouthia and Malaysian Hydrophis Schistosus: Insights Into Toxin-Specific Neutralization By Two Different Antivenoms." Toxins, vol. 8, no. 4, 2016, p. 86.
Tan KY, Tan CH, Fung SY, et al. Neutralization of the Principal Toxins from the Venoms of Thai Naja kaouthia and Malaysian Hydrophis schistosus: Insights into Toxin-Specific Neutralization by Two Different Antivenoms. Toxins (Basel). 2016;8(4):86.
Tan, K. Y., Tan, C. H., Fung, S. Y., & Tan, N. H. (2016). Neutralization of the Principal Toxins from the Venoms of Thai Naja kaouthia and Malaysian Hydrophis schistosus: Insights into Toxin-Specific Neutralization by Two Different Antivenoms. Toxins, 8(4), 86. https://doi.org/10.3390/toxins8040086
Tan KY, et al. Neutralization of the Principal Toxins From the Venoms of Thai Naja Kaouthia and Malaysian Hydrophis Schistosus: Insights Into Toxin-Specific Neutralization By Two Different Antivenoms. Toxins (Basel). 2016 Mar 26;8(4):86. PubMed PMID: 27023606.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neutralization of the Principal Toxins from the Venoms of Thai Naja kaouthia and Malaysian Hydrophis schistosus: Insights into Toxin-Specific Neutralization by Two Different Antivenoms. AU - Tan,Kae Yi, AU - Tan,Choo Hock, AU - Fung,Shin Yee, AU - Tan,Nget Hong, Y1 - 2016/03/26/ PY - 2016/01/08/received PY - 2016/03/06/revised PY - 2016/03/15/accepted PY - 2016/3/30/entrez PY - 2016/3/31/pubmed PY - 2016/12/16/medline KW - Naja kaouthia monovalent antivenom KW - antivenom potency KW - sea snake antivenom KW - toxin purification KW - toxin-specific neutralization SP - 86 EP - 86 JF - Toxins JO - Toxins (Basel) VL - 8 IS - 4 N2 - Antivenom neutralization against cobra venoms is generally low in potency, presumably due to poor toxin-specific immunoreactivity. This study aimed to investigate the effectiveness of two elapid antivenoms to neutralize the principal toxins purified from the venoms of the Thai monocled cobra (Naja kaouthia, Nk-T) and the Malaysian beaked sea snake (Hydrophis schistosus, Hs-M). In mice, N. kaouthia Monovalent Antivenom (NKMAV) neutralization against Nk-T long neurotoxin (LNTX) and cytotoxin was moderate (potency of 2.89-6.44 mg toxin/g antivenom protein) but poor against the short neurotoxin (SNTX) (1.33 mg/g). Its cross-neutralization against Hs-M LNTX of Hs-M is compatible (0.18 mg/g) but much weaker against Hs-M SNTX (0.22 mg/g). Using CSL (Seqirus Limited) Sea Snake Antivenom (SSAV), we observed consistently weak neutralization of antivenom against SNTX of both species, suggesting that this is the limiting factor on the potency of antivenom neutralization against venoms containing SNTX. Nevertheless, SSAV outperformed NKMAV in neutralizing SNTXs of both species (0.61-2.49 mg/g). The superior efficacy of SSAV against SNTX is probably partly attributable to the high abundance of SNTX in sea snake venom used as immunogen in SSAV production. The findings indicate that improving the potency of cobra antivenom may be possible with a proper immunogen formulation that seeks to overcome the limitation on SNTX immunoreactivity. SN - 2072-6651 UR - https://www.unboundmedicine.com/medline/citation/27023606/Neutralization_of_the_Principal_Toxins_from_the_Venoms_of_Thai_Naja_kaouthia_and_Malaysian_Hydrophis_schistosus:_Insights_into_Toxin_Specific_Neutralization_by_Two_Different_Antivenoms_ L2 - https://www.mdpi.com/resolver?pii=toxins8040086 DB - PRIME DP - Unbound Medicine ER -
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