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A new 2DS·2RL Robertsonian translocation transfers stem rust resistance gene Sr59 into wheat.
Theor Appl Genet. 2016 Jul; 129(7):1383-1392.TA

Abstract

KEY MESSAGE

A new stem rust resistance gene Sr59 from Secale cereale was introgressed into wheat as a 2DS·2RL Robertsonian translocation. Emerging new races of the wheat stem rust pathogen (Puccinia graminis f. sp. tritici), from Africa threaten global wheat (Triticum aestivum L.) production. To broaden the resistance spectrum of wheat to these widely virulent African races, additional resistance genes must be identified from all possible gene pools. From the screening of a collection of wheat-rye (Secale cereale L.) chromosome substitution lines developed at the Swedish University of Agricultural Sciences, we described the line 'SLU238' 2R (2D) as possessing resistance to many races of P. graminis f. sp. tritici, including the widely virulent race TTKSK (isolate synonym Ug99) from Africa. The breakage-fusion mechanism of univalent chromosomes was used to produce a new Robertsonian translocation: T2DS·2RL. Molecular marker analysis and stem rust seedling assays at multiple generations confirmed that the stem rust resistance from 'SLU238' is present on the rye chromosome arm 2RL. Line TA5094 (#101) was derived from 'SLU238' and was found to be homozygous for the T2DS·2RL translocation. The stem rust resistance gene on chromosome 2RL arm was designated as Sr59. Although introgressions of rye chromosome arms into wheat have most often been facilitated by irradiation, this study highlights the utility of the breakage-fusion mechanism for rye chromatin introgression. Sr59 provides an additional asset for wheat improvement to mitigate yield losses caused by stem rust.

Authors+Show Affiliations

Department of Plant Breeding, Swedish University of Agricultural Sciences, PO Box 101, 23053, Alnarp, Sweden. mahbubjon@gmail.com. Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA. mahbubjon@gmail.com. Tajik Agrarian University, 146, Rudaki Ave., Dushanbe, 734017, Tajikistan. mahbubjon@gmail.com.United States Department of Agriculture, Agricultural Research Service, Cereal Disease Laboratory, St. Paul, MN, 55108, USA. Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA.United States Department of Agriculture, Agricultural Research Service, Cereal Disease Laboratory, St. Paul, MN, 55108, USA.Department of Plant Pathology, Wheat Genetic Resources Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.Department of Plant Pathology, Wheat Genetic Resources Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA.Department of Plant Breeding, Swedish University of Agricultural Sciences, PO Box 101, 23053, Alnarp, Sweden.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27025509

Citation

Rahmatov, Mahbubjon, et al. "A New 2DS·2RL Robertsonian Translocation Transfers Stem Rust Resistance Gene Sr59 Into Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 129, no. 7, 2016, pp. 1383-1392.
Rahmatov M, Rouse MN, Nirmala J, et al. A new 2DS·2RL Robertsonian translocation transfers stem rust resistance gene Sr59 into wheat. Theor Appl Genet. 2016;129(7):1383-1392.
Rahmatov, M., Rouse, M. N., Nirmala, J., Danilova, T., Friebe, B., Steffenson, B. J., & Johansson, E. (2016). A new 2DS·2RL Robertsonian translocation transfers stem rust resistance gene Sr59 into wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 129(7), 1383-1392. https://doi.org/10.1007/s00122-016-2710-6
Rahmatov M, et al. A New 2DS·2RL Robertsonian Translocation Transfers Stem Rust Resistance Gene Sr59 Into Wheat. Theor Appl Genet. 2016;129(7):1383-1392. PubMed PMID: 27025509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new 2DS·2RL Robertsonian translocation transfers stem rust resistance gene Sr59 into wheat. AU - Rahmatov,Mahbubjon, AU - Rouse,Matthew N, AU - Nirmala,Jayaveeramuthu, AU - Danilova,Tatiana, AU - Friebe,Bernd, AU - Steffenson,Brian J, AU - Johansson,Eva, Y1 - 2016/03/29/ PY - 2016/01/23/received PY - 2016/03/17/accepted PY - 2016/3/31/entrez PY - 2016/3/31/pubmed PY - 2017/2/6/medline SP - 1383 EP - 1392 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 129 IS - 7 N2 - KEY MESSAGE: A new stem rust resistance gene Sr59 from Secale cereale was introgressed into wheat as a 2DS·2RL Robertsonian translocation. Emerging new races of the wheat stem rust pathogen (Puccinia graminis f. sp. tritici), from Africa threaten global wheat (Triticum aestivum L.) production. To broaden the resistance spectrum of wheat to these widely virulent African races, additional resistance genes must be identified from all possible gene pools. From the screening of a collection of wheat-rye (Secale cereale L.) chromosome substitution lines developed at the Swedish University of Agricultural Sciences, we described the line 'SLU238' 2R (2D) as possessing resistance to many races of P. graminis f. sp. tritici, including the widely virulent race TTKSK (isolate synonym Ug99) from Africa. The breakage-fusion mechanism of univalent chromosomes was used to produce a new Robertsonian translocation: T2DS·2RL. Molecular marker analysis and stem rust seedling assays at multiple generations confirmed that the stem rust resistance from 'SLU238' is present on the rye chromosome arm 2RL. Line TA5094 (#101) was derived from 'SLU238' and was found to be homozygous for the T2DS·2RL translocation. The stem rust resistance gene on chromosome 2RL arm was designated as Sr59. Although introgressions of rye chromosome arms into wheat have most often been facilitated by irradiation, this study highlights the utility of the breakage-fusion mechanism for rye chromatin introgression. Sr59 provides an additional asset for wheat improvement to mitigate yield losses caused by stem rust. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/27025509/A_new_2DS·2RL_Robertsonian_translocation_transfers_stem_rust_resistance_gene_Sr59_into_wheat_ L2 - https://dx.doi.org/10.1007/s00122-016-2710-6 DB - PRIME DP - Unbound Medicine ER -