Tags

Type your tag names separated by a space and hit enter

Regulation of transient receptor potential vanilloid 1 expression in trigeminal ganglion neurons via methyl-CpG binding protein 2 signaling contributes tongue heat sensitivity and inflammatory hyperalgesia in mice.
Mol Pain. 2016; 12MP

Abstract

BACKGROUND

Pain hypoalgesia has been reported in Rett syndrome patients, a severe neurodevelopmental disorder which can be attributed to mutations in the methyl-CpG binding protein 2 (MeCP2). Here, we examined the role of MeCP2 signaling in tongue heat sensitivity in the normal and inflamed state using Mecp2 heterozygous (Mecp2(+/-)) mice.

RESULTS

Heat hypoalgesia of the tongue occurred in Mecp2(+/-) mice and submucosal injection of complete Freund's adjuvant into the tongue produced a long-lasting heat hyperalgesia at the inflamed site in wild-type mice but not in Mecp2(+/-) mice. Transient receptor potential vanilloid 1 was expressed in a large number of MeCP2-immunoreactive trigeminal ganglion neurons innervating the tongue in both wild-type and Mecp2(+/-) mice (70.9% in wild type; 72.1% in Mecp2(+/-)). The number of transient receptor potential vanilloid 1-immunoreactive trigeminal ganglion neurons innervating the tongue was smaller in Mecp2(+/-) mice relative to wild-type mice (30.5% in wild type; 20.2% in Mecp2(+/-)). Following complete Freund's adjuvant injection, the number of transient receptor potential vanilloid 1- and MeCP2-immunoreactive trigeminal ganglion neurons innervating the tongue, as well as MeCP2 protein expression in trigeminal ganglion, was significantly increased in wild-type mice but not in Mecp2(+/-) mice. Additionally, tongue heat hyperalgesia following complete Freund's adjuvant injection was completely suppressed by the administration of SB366791, a transient receptor potential vanilloid 1 antagonist, in the tongue.

CONCLUSIONS

These findings indicate that tongue heat sensitivity and hypersensitivity are dependent on the expression of transient receptor potential vanilloid 1 which is regulated via MeCP2 signaling in trigeminal ganglion neurons innervating the tongue.

Authors+Show Affiliations

Department of Pediatric Dentistry, Nihon University School of Dentistry, Chiyoda-ku, Tokyo, Japan.Department of Physiology, Nihon University School of Dentistry, Chiyoda-ku, Tokyo, Japan shinoda.masamichi@nihon-u.ac.jp.Department of Physiology, Nihon University School of Dentistry, Chiyoda-ku, Tokyo, Japan.Department of Pediatric Dentistry, Nihon University School of Dentistry, Chiyoda-ku, Tokyo, Japan.Department of Physiology, Nihon University School of Dentistry, Chiyoda-ku, Tokyo, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27030715

Citation

Suzuki, Azumi, et al. "Regulation of Transient Receptor Potential Vanilloid 1 Expression in Trigeminal Ganglion Neurons Via methyl-CpG Binding Protein 2 Signaling Contributes Tongue Heat Sensitivity and Inflammatory Hyperalgesia in Mice." Molecular Pain, vol. 12, 2016.
Suzuki A, Shinoda M, Honda K, et al. Regulation of transient receptor potential vanilloid 1 expression in trigeminal ganglion neurons via methyl-CpG binding protein 2 signaling contributes tongue heat sensitivity and inflammatory hyperalgesia in mice. Mol Pain. 2016;12.
Suzuki, A., Shinoda, M., Honda, K., Shirakawa, T., & Iwata, K. (2016). Regulation of transient receptor potential vanilloid 1 expression in trigeminal ganglion neurons via methyl-CpG binding protein 2 signaling contributes tongue heat sensitivity and inflammatory hyperalgesia in mice. Molecular Pain, 12. https://doi.org/10.1177/1744806916633206
Suzuki A, et al. Regulation of Transient Receptor Potential Vanilloid 1 Expression in Trigeminal Ganglion Neurons Via methyl-CpG Binding Protein 2 Signaling Contributes Tongue Heat Sensitivity and Inflammatory Hyperalgesia in Mice. Mol Pain. 2016;12 PubMed PMID: 27030715.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of transient receptor potential vanilloid 1 expression in trigeminal ganglion neurons via methyl-CpG binding protein 2 signaling contributes tongue heat sensitivity and inflammatory hyperalgesia in mice. AU - Suzuki,Azumi, AU - Shinoda,Masamichi, AU - Honda,Kuniya, AU - Shirakawa,Tetsuro, AU - Iwata,Koichi, Y1 - 2016/03/08/ PY - 2015/12/21/received PY - 2015/12/29/accepted PY - 2016/4/1/entrez PY - 2016/4/1/pubmed PY - 2016/12/20/medline KW - Rett syndrome KW - Trigeminal ganglion KW - tongue inflammation KW - transient receptor potential vanilloid 1 JF - Molecular pain JO - Mol Pain VL - 12 N2 - BACKGROUND: Pain hypoalgesia has been reported in Rett syndrome patients, a severe neurodevelopmental disorder which can be attributed to mutations in the methyl-CpG binding protein 2 (MeCP2). Here, we examined the role of MeCP2 signaling in tongue heat sensitivity in the normal and inflamed state using Mecp2 heterozygous (Mecp2(+/-)) mice. RESULTS: Heat hypoalgesia of the tongue occurred in Mecp2(+/-) mice and submucosal injection of complete Freund's adjuvant into the tongue produced a long-lasting heat hyperalgesia at the inflamed site in wild-type mice but not in Mecp2(+/-) mice. Transient receptor potential vanilloid 1 was expressed in a large number of MeCP2-immunoreactive trigeminal ganglion neurons innervating the tongue in both wild-type and Mecp2(+/-) mice (70.9% in wild type; 72.1% in Mecp2(+/-)). The number of transient receptor potential vanilloid 1-immunoreactive trigeminal ganglion neurons innervating the tongue was smaller in Mecp2(+/-) mice relative to wild-type mice (30.5% in wild type; 20.2% in Mecp2(+/-)). Following complete Freund's adjuvant injection, the number of transient receptor potential vanilloid 1- and MeCP2-immunoreactive trigeminal ganglion neurons innervating the tongue, as well as MeCP2 protein expression in trigeminal ganglion, was significantly increased in wild-type mice but not in Mecp2(+/-) mice. Additionally, tongue heat hyperalgesia following complete Freund's adjuvant injection was completely suppressed by the administration of SB366791, a transient receptor potential vanilloid 1 antagonist, in the tongue. CONCLUSIONS: These findings indicate that tongue heat sensitivity and hypersensitivity are dependent on the expression of transient receptor potential vanilloid 1 which is regulated via MeCP2 signaling in trigeminal ganglion neurons innervating the tongue. SN - 1744-8069 UR - https://www.unboundmedicine.com/medline/citation/27030715/Regulation_of_transient_receptor_potential_vanilloid_1_expression_in_trigeminal_ganglion_neurons_via_methyl_CpG_binding_protein_2_signaling_contributes_tongue_heat_sensitivity_and_inflammatory_hyperalgesia_in_mice_ L2 - https://journals.sagepub.com/doi/10.1177/1744806916633206?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -