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Turn-on near-infrared electrochemiluminescence sensing of thrombin based on resonance energy transfer between CdTe/CdS coresmall/shellthick quantum dots and gold nanorods.
Biosens Bioelectron. 2016 Aug 15; 82:26-31.BB

Abstract

Here we designed a near-infrared electrochemiluminescence (NECL) aptasensor for turn-on ultrasensitive determination of thrombin. It was based on the ECL resonance energy transfer (ECL-RET) of CdTe/CdS coresmall/shellthick quantum dots (QDs) to gold nanorods (AuNRs). AuNRs which functioned as ECL acceptors were assembled onto CdTe/CdS film by DNA hybridization between aptamers and their complementary oligonucleotides. In the absence of thrombin, the NECL of QDs was quenched as a result of the ECL-RET of QDs to AuNRs. In the presence of thrombin, the NECL of the system was "turned on" because thrombin can replace the AuNRs onto the QDs film, owing to the specific aptamer-protein affinity interactions. In this way, the increment of ECL intensity and the concentration of thrombin showed a logarithmic linear correlation in the range of 100 aM to 10 fM with a detection limit of 31 aM (S/N=3). Importantly, the developed aptasensor was successfully applied to thrombin sensing in real serum samples.

Authors+Show Affiliations

State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China; College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China.State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China; Hubei Key Laboratory for Processing and Application of Catalytic Materials, College of Chemical Engineering, Huanggang Normal University, Huangzhou 438000, PR China.State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: hyhan@mail.hzau.edu.cn.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

27031188

Citation

Wang, Jing, et al. "Turn-on Near-infrared Electrochemiluminescence Sensing of Thrombin Based On Resonance Energy Transfer Between CdTe/CdS Coresmall/shellthick Quantum Dots and Gold Nanorods." Biosensors & Bioelectronics, vol. 82, 2016, pp. 26-31.
Wang J, Jiang X, Han H. Turn-on near-infrared electrochemiluminescence sensing of thrombin based on resonance energy transfer between CdTe/CdS coresmall/shellthick quantum dots and gold nanorods. Biosens Bioelectron. 2016;82:26-31.
Wang, J., Jiang, X., & Han, H. (2016). Turn-on near-infrared electrochemiluminescence sensing of thrombin based on resonance energy transfer between CdTe/CdS coresmall/shellthick quantum dots and gold nanorods. Biosensors & Bioelectronics, 82, 26-31. https://doi.org/10.1016/j.bios.2016.03.057
Wang J, Jiang X, Han H. Turn-on Near-infrared Electrochemiluminescence Sensing of Thrombin Based On Resonance Energy Transfer Between CdTe/CdS Coresmall/shellthick Quantum Dots and Gold Nanorods. Biosens Bioelectron. 2016 Aug 15;82:26-31. PubMed PMID: 27031188.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Turn-on near-infrared electrochemiluminescence sensing of thrombin based on resonance energy transfer between CdTe/CdS coresmall/shellthick quantum dots and gold nanorods. AU - Wang,Jing, AU - Jiang,Xiaochun, AU - Han,Heyou, Y1 - 2016/03/24/ PY - 2016/01/15/received PY - 2016/03/13/revised PY - 2016/03/22/accepted PY - 2016/4/1/entrez PY - 2016/4/1/pubmed PY - 2017/1/24/medline KW - Aptasensor KW - CdTe/CdS quantum dots KW - Electrochemiluminescence resonance energy transfer KW - Gold nanorods KW - Near-infrared KW - Thrombin SP - 26 EP - 31 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 82 N2 - Here we designed a near-infrared electrochemiluminescence (NECL) aptasensor for turn-on ultrasensitive determination of thrombin. It was based on the ECL resonance energy transfer (ECL-RET) of CdTe/CdS coresmall/shellthick quantum dots (QDs) to gold nanorods (AuNRs). AuNRs which functioned as ECL acceptors were assembled onto CdTe/CdS film by DNA hybridization between aptamers and their complementary oligonucleotides. In the absence of thrombin, the NECL of QDs was quenched as a result of the ECL-RET of QDs to AuNRs. In the presence of thrombin, the NECL of the system was "turned on" because thrombin can replace the AuNRs onto the QDs film, owing to the specific aptamer-protein affinity interactions. In this way, the increment of ECL intensity and the concentration of thrombin showed a logarithmic linear correlation in the range of 100 aM to 10 fM with a detection limit of 31 aM (S/N=3). Importantly, the developed aptasensor was successfully applied to thrombin sensing in real serum samples. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/27031188/Turn_on_near_infrared_electrochemiluminescence_sensing_of_thrombin_based_on_resonance_energy_transfer_between_CdTe/CdS_coresmall/shellthick_quantum_dots_and_gold_nanorods_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(16)30248-2 DB - PRIME DP - Unbound Medicine ER -