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Curcumin inhibits hypoxia-induced epithelial‑mesenchymal transition in pancreatic cancer cells via suppression of the hedgehog signaling pathway.
Oncol Rep. 2016 Jun; 35(6):3728-34.OR

Abstract

Hypoxic microenvironment, a common feature of pancreatic cancer, is associated with tumor proliferation, metastasis and epithelial-mesenchymal transition (EMT) changes. In recent years, many natural agents, including curcumin, have been proven to possess the ability to inhibit the progression of pancreatic cancer. However, whether curcumin is able to suppress hypoxia-induced pancreatic cancer progression and the underlying mechanisms are still not fully elucidated. The aim of the present study was to evaluate whether curcumin affects hypoxia-induced EMT and the activation of Hh signaling pathway in pancreatic cancer. The human pancreatic cancer cell line Panc-1, was treated with hypoxic condition and curcumin. Cell proliferation was assessed by the MTT assay. Wound healing assay and transwell invasion assay were used to detect the migratory and invasive activity of cancer cells. The EMT-related factors, E-cadherin, N-cadherin, vimentin were detected by QT-PCR, western blot analysis and immunofluorescence staining. The Hh signaling-related factors, SHH, SMO and GLI1 were detected by western blot analysis. The results of present study showed that curcumin could not only inhibit the hypoxia-induced cell proliferation, migration and invasion in pancreatic cancer, but also mediate the expression of EMT-related factors. In addition, curcumin remarkably inhibited hypoxia-mediated activation of Hh signaling pathway. Taken together, these data indicate that curcumin plays an important role in suppressing hypoxia-induced pancreatic cancer metastasis by inhibiting the Hh signaling pathway. Curcumin might be a potential candidate for chemoprevention of this severe disease.

Authors+Show Affiliations

Department of Pharmacology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, P.R. China.Department of Pharmacology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, P.R. China.Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27035865

Citation

Cao, Lei, et al. "Curcumin Inhibits Hypoxia-induced Epithelial‑mesenchymal Transition in Pancreatic Cancer Cells Via Suppression of the Hedgehog Signaling Pathway." Oncology Reports, vol. 35, no. 6, 2016, pp. 3728-34.
Cao L, Xiao X, Lei J, et al. Curcumin inhibits hypoxia-induced epithelial‑mesenchymal transition in pancreatic cancer cells via suppression of the hedgehog signaling pathway. Oncol Rep. 2016;35(6):3728-34.
Cao, L., Xiao, X., Lei, J., Duan, W., Ma, Q., & Li, W. (2016). Curcumin inhibits hypoxia-induced epithelial‑mesenchymal transition in pancreatic cancer cells via suppression of the hedgehog signaling pathway. Oncology Reports, 35(6), 3728-34. https://doi.org/10.3892/or.2016.4709
Cao L, et al. Curcumin Inhibits Hypoxia-induced Epithelial‑mesenchymal Transition in Pancreatic Cancer Cells Via Suppression of the Hedgehog Signaling Pathway. Oncol Rep. 2016;35(6):3728-34. PubMed PMID: 27035865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Curcumin inhibits hypoxia-induced epithelial‑mesenchymal transition in pancreatic cancer cells via suppression of the hedgehog signaling pathway. AU - Cao,Lei, AU - Xiao,Xue, AU - Lei,Jianjun, AU - Duan,Wanxing, AU - Ma,Qingyong, AU - Li,Wei, Y1 - 2016/03/28/ PY - 2015/12/23/received PY - 2016/02/11/accepted PY - 2016/4/2/entrez PY - 2016/4/2/pubmed PY - 2017/3/14/medline SP - 3728 EP - 34 JF - Oncology reports JO - Oncol Rep VL - 35 IS - 6 N2 - Hypoxic microenvironment, a common feature of pancreatic cancer, is associated with tumor proliferation, metastasis and epithelial-mesenchymal transition (EMT) changes. In recent years, many natural agents, including curcumin, have been proven to possess the ability to inhibit the progression of pancreatic cancer. However, whether curcumin is able to suppress hypoxia-induced pancreatic cancer progression and the underlying mechanisms are still not fully elucidated. The aim of the present study was to evaluate whether curcumin affects hypoxia-induced EMT and the activation of Hh signaling pathway in pancreatic cancer. The human pancreatic cancer cell line Panc-1, was treated with hypoxic condition and curcumin. Cell proliferation was assessed by the MTT assay. Wound healing assay and transwell invasion assay were used to detect the migratory and invasive activity of cancer cells. The EMT-related factors, E-cadherin, N-cadherin, vimentin were detected by QT-PCR, western blot analysis and immunofluorescence staining. The Hh signaling-related factors, SHH, SMO and GLI1 were detected by western blot analysis. The results of present study showed that curcumin could not only inhibit the hypoxia-induced cell proliferation, migration and invasion in pancreatic cancer, but also mediate the expression of EMT-related factors. In addition, curcumin remarkably inhibited hypoxia-mediated activation of Hh signaling pathway. Taken together, these data indicate that curcumin plays an important role in suppressing hypoxia-induced pancreatic cancer metastasis by inhibiting the Hh signaling pathway. Curcumin might be a potential candidate for chemoprevention of this severe disease. SN - 1791-2431 UR - https://www.unboundmedicine.com/medline/citation/27035865/Curcumin_inhibits_hypoxia_induced_epithelial‑mesenchymal_transition_in_pancreatic_cancer_cells_via_suppression_of_the_hedgehog_signaling_pathway_ L2 - http://www.spandidos-publications.com/or/35/6/3728 DB - PRIME DP - Unbound Medicine ER -