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A loss-of-function allele of OsHMA3 associated with high cadmium accumulation in shoots and grain of Japonica rice cultivars.
Plant Cell Environ. 2016 09; 39(9):1941-54.PC

Abstract

Excessive cadmium (Cd) accumulation in rice poses a risk to food safety. OsHMA3 plays an important role in restricting Cd translocation from roots to shoots. A non-functional allele of OsHMA3 has been reported in some Indica rice cultivars with high Cd accumulation, but it is not known if OsHMA3 allelic variation is associated with Cd accumulation in Japonica cultivars. In this study, we identified a Japonica cultivar with consistently high Cd accumulation in shoots and grain in both field and greenhouse experiments. The cultivar possesses an OsHMA3 allele with a predicted amino acid mutation at the 380(th) position from Ser to Arg. The haplotype had no Cd transport activity when the gene was expressed in yeast, and the allele did not complement a known nonfunctional allele of OsHMA3 in F1 test. The allele is present only in temperate Japonica cultivars among diversity panels of 1483 rice cultivars. Different cultivars possessing this allele showed greatly increased root-to-shoot Cd translocation and a shift in root Cd speciation from Cd-S to Cd-O bonding determined by synchrotron X-ray absorption spectroscopy. Our study has identified a new loss-of-function allele of OsHMA3 in Japonica rice cultivars leading to high Cd accumulation in shoots and grain.

Authors+Show Affiliations

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. School of Agriculture and Food Sciences, Faculty of Science, The University of Queensland, St Lucia, Queensland, 4072, Australia.National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.Institute of Biological and Environmental Sciences, University of Aberdeen, Cruickshank Building, St. Machar Drive, Aberdeen, UK.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27038090

Citation

Yan, Jiali, et al. "A Loss-of-function Allele of OsHMA3 Associated With High Cadmium Accumulation in Shoots and Grain of Japonica Rice Cultivars." Plant, Cell & Environment, vol. 39, no. 9, 2016, pp. 1941-54.
Yan J, Wang P, Wang P, et al. A loss-of-function allele of OsHMA3 associated with high cadmium accumulation in shoots and grain of Japonica rice cultivars. Plant Cell Environ. 2016;39(9):1941-54.
Yan, J., Wang, P., Wang, P., Yang, M., Lian, X., Tang, Z., Huang, C. F., Salt, D. E., & Zhao, F. J. (2016). A loss-of-function allele of OsHMA3 associated with high cadmium accumulation in shoots and grain of Japonica rice cultivars. Plant, Cell & Environment, 39(9), 1941-54. https://doi.org/10.1111/pce.12747
Yan J, et al. A Loss-of-function Allele of OsHMA3 Associated With High Cadmium Accumulation in Shoots and Grain of Japonica Rice Cultivars. Plant Cell Environ. 2016;39(9):1941-54. PubMed PMID: 27038090.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A loss-of-function allele of OsHMA3 associated with high cadmium accumulation in shoots and grain of Japonica rice cultivars. AU - Yan,Jiali, AU - Wang,Peitong, AU - Wang,Peng, AU - Yang,Meng, AU - Lian,Xingming, AU - Tang,Zhong, AU - Huang,Chao-Feng, AU - Salt,David E, AU - Zhao,Fang Jie, Y1 - 2016/06/16/ PY - 2015/11/18/received PY - 2016/03/20/revised PY - 2016/03/24/accepted PY - 2016/4/3/entrez PY - 2016/4/3/pubmed PY - 2017/12/16/medline KW - Cd speciation KW - Oryza sativa (rice) KW - allelic variation KW - translocation SP - 1941 EP - 54 JF - Plant, cell & environment JO - Plant Cell Environ VL - 39 IS - 9 N2 - Excessive cadmium (Cd) accumulation in rice poses a risk to food safety. OsHMA3 plays an important role in restricting Cd translocation from roots to shoots. A non-functional allele of OsHMA3 has been reported in some Indica rice cultivars with high Cd accumulation, but it is not known if OsHMA3 allelic variation is associated with Cd accumulation in Japonica cultivars. In this study, we identified a Japonica cultivar with consistently high Cd accumulation in shoots and grain in both field and greenhouse experiments. The cultivar possesses an OsHMA3 allele with a predicted amino acid mutation at the 380(th) position from Ser to Arg. The haplotype had no Cd transport activity when the gene was expressed in yeast, and the allele did not complement a known nonfunctional allele of OsHMA3 in F1 test. The allele is present only in temperate Japonica cultivars among diversity panels of 1483 rice cultivars. Different cultivars possessing this allele showed greatly increased root-to-shoot Cd translocation and a shift in root Cd speciation from Cd-S to Cd-O bonding determined by synchrotron X-ray absorption spectroscopy. Our study has identified a new loss-of-function allele of OsHMA3 in Japonica rice cultivars leading to high Cd accumulation in shoots and grain. SN - 1365-3040 UR - https://www.unboundmedicine.com/medline/citation/27038090/A_loss_of_function_allele_of_OsHMA3_associated_with_high_cadmium_accumulation_in_shoots_and_grain_of_Japonica_rice_cultivars_ DB - PRIME DP - Unbound Medicine ER -